Molecular Characterization of Aureobasidium Species in Iran
Background: Members of this genus Aureobasidium are ubiquitous microorganisms which can be isolated from wide ranges of substrates such as plant materials (phyllosphere, plant debris, bark, roots, fruits and wood), soil, dead wood, air, and as rare etiologic agent of pheohyphomycosis, keratomycosis,...
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Format: | Article |
Language: | English |
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Mazandaran University of Medical Sciences and Health Services
2014-05-01
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Series: | Research in Molecular Medicine |
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Online Access: | http://rmm.mazums.ac.ir/browse.php?a_code=A-10-26-22&slc_lang=en&sid=1 |
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author | Mahdi Arzanlou |
author_facet | Mahdi Arzanlou |
author_sort | Mahdi Arzanlou |
collection | DOAJ |
description | Background: Members of this genus Aureobasidium are ubiquitous microorganisms which can be isolated from wide ranges of substrates such as plant materials (phyllosphere, plant debris, bark, roots, fruits and wood), soil, dead wood, air, and as rare etiologic agent of pheohyphomycosis, keratomycosis, septicemia, peritoneal sepsis, and dermatological infections in human. Very little is known on the identity, substrates and distribution of Aureobasidium spp. in Iran. Materials and Methods: Fourteen Aureobasidium isolates were recovered from vascular tissues of pome and stone fruit trees displaying decline symptoms in orchards of West and East Azarbaijan provinces, Iran. Pure cultures were established by using a single spore technique. The identity of the isolates was determined using sequence data from ITS-rDNA region. Phylogenetic relationship among isolates was inferred based on sequence data from ITS-rDNA. Results: A megablast search analysis of ITS sequence data at NCBI revealed the identity of Aureobasidium isolates as A. pullulans. A phylogeny inferred using sequence data from ITS region placed our isolates together with the other A. pullulans var. pullulans in GenBank. Morphological and cultural characteristics were in agreement with the description for A. pullulans var. pullulans. Conclusion: Our results represent new report on the occurrence of A. pullulans var. pullulans in Iran. As A. pullulans is known as a rare etiologic agent of pheohyphomycosis, keratomycosis, septicemia, peritoneal sepsis, and dermatological infections in human, possible occurrence and involvement of A. pullulans in human infections should be taken into account. |
first_indexed | 2024-12-20T21:13:15Z |
format | Article |
id | doaj.art-d3ec6959b5264a29b928df256caef25b |
institution | Directory Open Access Journal |
issn | 2322-1348 2322-133X |
language | English |
last_indexed | 2024-12-20T21:13:15Z |
publishDate | 2014-05-01 |
publisher | Mazandaran University of Medical Sciences and Health Services |
record_format | Article |
series | Research in Molecular Medicine |
spelling | doaj.art-d3ec6959b5264a29b928df256caef25b2022-12-21T19:26:29ZengMazandaran University of Medical Sciences and Health ServicesResearch in Molecular Medicine2322-13482322-133X2014-05-01222833Molecular Characterization of Aureobasidium Species in IranMahdi Arzanlou0 Mycology and Plant Pathology Background: Members of this genus Aureobasidium are ubiquitous microorganisms which can be isolated from wide ranges of substrates such as plant materials (phyllosphere, plant debris, bark, roots, fruits and wood), soil, dead wood, air, and as rare etiologic agent of pheohyphomycosis, keratomycosis, septicemia, peritoneal sepsis, and dermatological infections in human. Very little is known on the identity, substrates and distribution of Aureobasidium spp. in Iran. Materials and Methods: Fourteen Aureobasidium isolates were recovered from vascular tissues of pome and stone fruit trees displaying decline symptoms in orchards of West and East Azarbaijan provinces, Iran. Pure cultures were established by using a single spore technique. The identity of the isolates was determined using sequence data from ITS-rDNA region. Phylogenetic relationship among isolates was inferred based on sequence data from ITS-rDNA. Results: A megablast search analysis of ITS sequence data at NCBI revealed the identity of Aureobasidium isolates as A. pullulans. A phylogeny inferred using sequence data from ITS region placed our isolates together with the other A. pullulans var. pullulans in GenBank. Morphological and cultural characteristics were in agreement with the description for A. pullulans var. pullulans. Conclusion: Our results represent new report on the occurrence of A. pullulans var. pullulans in Iran. As A. pullulans is known as a rare etiologic agent of pheohyphomycosis, keratomycosis, septicemia, peritoneal sepsis, and dermatological infections in human, possible occurrence and involvement of A. pullulans in human infections should be taken into account.http://rmm.mazums.ac.ir/browse.php?a_code=A-10-26-22&slc_lang=en&sid=1ITS-rDNA Endophytes Black yeast Pullulan Human pathogen |
spellingShingle | Mahdi Arzanlou Molecular Characterization of Aureobasidium Species in Iran Research in Molecular Medicine ITS-rDNA Endophytes Black yeast Pullulan Human pathogen |
title | Molecular Characterization of Aureobasidium Species in Iran |
title_full | Molecular Characterization of Aureobasidium Species in Iran |
title_fullStr | Molecular Characterization of Aureobasidium Species in Iran |
title_full_unstemmed | Molecular Characterization of Aureobasidium Species in Iran |
title_short | Molecular Characterization of Aureobasidium Species in Iran |
title_sort | molecular characterization of aureobasidium species in iran |
topic | ITS-rDNA Endophytes Black yeast Pullulan Human pathogen |
url | http://rmm.mazums.ac.ir/browse.php?a_code=A-10-26-22&slc_lang=en&sid=1 |
work_keys_str_mv | AT mahdiarzanlou molecularcharacterizationofaureobasidiumspeciesiniran |