X-treme loss of sequence diversity linked to neo-X chromosomes in filarial nematodes.
The sequence diversity of natural and laboratory populations of Brugia pahangi and Brugia malayi was assessed with Illumina resequencing followed by mapping in order to identify single nucleotide variants and insertions/deletions. In natural and laboratory Brugia populations, there is a lack of sequ...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-10-01
|
| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://doi.org/10.1371/journal.pntd.0009838 |
| _version_ | 1819177711577333760 |
|---|---|
| author | John Mattick Silvia Libro Robin Bromley Wanpen Chaicumpa Matthew Chung Darren Cook Mohammad Behram Khan Nikhil Kumar Yee-Ling Lau Shailja Misra-Bhattacharya Ramakrishna Rao Lisa Sadzewicz Atiporn Saeung Mohd Shahab Benjamin C Sparklin Andrew Steven Joseph D Turner Luke J Tallon Mark J Taylor Andrew R Moorhead Michelle Michalski Jeremy M Foster Julie C Dunning Hotopp |
| author_facet | John Mattick Silvia Libro Robin Bromley Wanpen Chaicumpa Matthew Chung Darren Cook Mohammad Behram Khan Nikhil Kumar Yee-Ling Lau Shailja Misra-Bhattacharya Ramakrishna Rao Lisa Sadzewicz Atiporn Saeung Mohd Shahab Benjamin C Sparklin Andrew Steven Joseph D Turner Luke J Tallon Mark J Taylor Andrew R Moorhead Michelle Michalski Jeremy M Foster Julie C Dunning Hotopp |
| author_sort | John Mattick |
| collection | DOAJ |
| description | The sequence diversity of natural and laboratory populations of Brugia pahangi and Brugia malayi was assessed with Illumina resequencing followed by mapping in order to identify single nucleotide variants and insertions/deletions. In natural and laboratory Brugia populations, there is a lack of sequence diversity on chromosome X relative to the autosomes (πX/πA = 0.2), which is lower than the expected (πX/πA = 0.75). A reduction in diversity is also observed in other filarial nematodes with neo-X chromosome fusions in the genera Onchocerca and Wuchereria, but not those without neo-X chromosome fusions in the genera Loa and Dirofilaria. In the species with neo-X chromosome fusions, chromosome X is abnormally large, containing a third of the genetic material such that a sizable portion of the genome is lacking sequence diversity. Such profound differences in genetic diversity can be consequential, having been associated with drug resistance and adaptability, with the potential to affect filarial eradication. |
| first_indexed | 2024-12-22T21:31:00Z |
| format | Article |
| id | doaj.art-d3ed3b4ffa6f46c2846930745f0b264f |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2024-12-22T21:31:00Z |
| publishDate | 2021-10-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-d3ed3b4ffa6f46c2846930745f0b264f2022-12-21T18:11:55ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352021-10-011510e000983810.1371/journal.pntd.0009838X-treme loss of sequence diversity linked to neo-X chromosomes in filarial nematodes.John MattickSilvia LibroRobin BromleyWanpen ChaicumpaMatthew ChungDarren CookMohammad Behram KhanNikhil KumarYee-Ling LauShailja Misra-BhattacharyaRamakrishna RaoLisa SadzewiczAtiporn SaeungMohd ShahabBenjamin C SparklinAndrew StevenJoseph D TurnerLuke J TallonMark J TaylorAndrew R MoorheadMichelle MichalskiJeremy M FosterJulie C Dunning HotoppThe sequence diversity of natural and laboratory populations of Brugia pahangi and Brugia malayi was assessed with Illumina resequencing followed by mapping in order to identify single nucleotide variants and insertions/deletions. In natural and laboratory Brugia populations, there is a lack of sequence diversity on chromosome X relative to the autosomes (πX/πA = 0.2), which is lower than the expected (πX/πA = 0.75). A reduction in diversity is also observed in other filarial nematodes with neo-X chromosome fusions in the genera Onchocerca and Wuchereria, but not those without neo-X chromosome fusions in the genera Loa and Dirofilaria. In the species with neo-X chromosome fusions, chromosome X is abnormally large, containing a third of the genetic material such that a sizable portion of the genome is lacking sequence diversity. Such profound differences in genetic diversity can be consequential, having been associated with drug resistance and adaptability, with the potential to affect filarial eradication.https://doi.org/10.1371/journal.pntd.0009838 |
| spellingShingle | John Mattick Silvia Libro Robin Bromley Wanpen Chaicumpa Matthew Chung Darren Cook Mohammad Behram Khan Nikhil Kumar Yee-Ling Lau Shailja Misra-Bhattacharya Ramakrishna Rao Lisa Sadzewicz Atiporn Saeung Mohd Shahab Benjamin C Sparklin Andrew Steven Joseph D Turner Luke J Tallon Mark J Taylor Andrew R Moorhead Michelle Michalski Jeremy M Foster Julie C Dunning Hotopp X-treme loss of sequence diversity linked to neo-X chromosomes in filarial nematodes. PLoS Neglected Tropical Diseases |
| title | X-treme loss of sequence diversity linked to neo-X chromosomes in filarial nematodes. |
| title_full | X-treme loss of sequence diversity linked to neo-X chromosomes in filarial nematodes. |
| title_fullStr | X-treme loss of sequence diversity linked to neo-X chromosomes in filarial nematodes. |
| title_full_unstemmed | X-treme loss of sequence diversity linked to neo-X chromosomes in filarial nematodes. |
| title_short | X-treme loss of sequence diversity linked to neo-X chromosomes in filarial nematodes. |
| title_sort | x treme loss of sequence diversity linked to neo x chromosomes in filarial nematodes |
| url | https://doi.org/10.1371/journal.pntd.0009838 |
| work_keys_str_mv | AT johnmattick xtremelossofsequencediversitylinkedtoneoxchromosomesinfilarialnematodes AT silvialibro xtremelossofsequencediversitylinkedtoneoxchromosomesinfilarialnematodes AT robinbromley xtremelossofsequencediversitylinkedtoneoxchromosomesinfilarialnematodes AT wanpenchaicumpa xtremelossofsequencediversitylinkedtoneoxchromosomesinfilarialnematodes AT matthewchung xtremelossofsequencediversitylinkedtoneoxchromosomesinfilarialnematodes AT darrencook xtremelossofsequencediversitylinkedtoneoxchromosomesinfilarialnematodes AT mohammadbehramkhan xtremelossofsequencediversitylinkedtoneoxchromosomesinfilarialnematodes AT nikhilkumar xtremelossofsequencediversitylinkedtoneoxchromosomesinfilarialnematodes AT yeelinglau xtremelossofsequencediversitylinkedtoneoxchromosomesinfilarialnematodes AT shailjamisrabhattacharya xtremelossofsequencediversitylinkedtoneoxchromosomesinfilarialnematodes AT ramakrishnarao xtremelossofsequencediversitylinkedtoneoxchromosomesinfilarialnematodes AT lisasadzewicz xtremelossofsequencediversitylinkedtoneoxchromosomesinfilarialnematodes AT atipornsaeung xtremelossofsequencediversitylinkedtoneoxchromosomesinfilarialnematodes AT mohdshahab xtremelossofsequencediversitylinkedtoneoxchromosomesinfilarialnematodes AT benjamincsparklin xtremelossofsequencediversitylinkedtoneoxchromosomesinfilarialnematodes AT andrewsteven xtremelossofsequencediversitylinkedtoneoxchromosomesinfilarialnematodes AT josephdturner xtremelossofsequencediversitylinkedtoneoxchromosomesinfilarialnematodes AT lukejtallon xtremelossofsequencediversitylinkedtoneoxchromosomesinfilarialnematodes AT markjtaylor xtremelossofsequencediversitylinkedtoneoxchromosomesinfilarialnematodes AT andrewrmoorhead xtremelossofsequencediversitylinkedtoneoxchromosomesinfilarialnematodes AT michellemichalski xtremelossofsequencediversitylinkedtoneoxchromosomesinfilarialnematodes AT jeremymfoster xtremelossofsequencediversitylinkedtoneoxchromosomesinfilarialnematodes AT juliecdunninghotopp xtremelossofsequencediversitylinkedtoneoxchromosomesinfilarialnematodes |