Histological and Molecular Evidence of the Positive Performance of Glycerol-Plasticized Chitosan-Alginate Membranes on Skin Lesions of Hyperglycemic Mice
The purpose of this study was to investigate tissue repair of excisional wounds in hyperglycemic animals treated with chitosan-alginate membranes (CAM) produced in the presence of glycerol. 8-week C57B1 male mice were divided into normoglycemic animals with a 0.9% saline solution topical treatment (...
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MDPI AG
2022-11-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/21/4754 |
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| author | Flavia Figueiredo Azevedo Thiago Anselmo Cantarutti Paula de Freitas Rosa Remiro Beatriz Barbieri Rafael Abboud Azoubel Mariana Harue Taniguchi Nagahara Ângela Maria Moraes Maria Helena Melo Lima |
| author_facet | Flavia Figueiredo Azevedo Thiago Anselmo Cantarutti Paula de Freitas Rosa Remiro Beatriz Barbieri Rafael Abboud Azoubel Mariana Harue Taniguchi Nagahara Ângela Maria Moraes Maria Helena Melo Lima |
| author_sort | Flavia Figueiredo Azevedo |
| collection | DOAJ |
| description | The purpose of this study was to investigate tissue repair of excisional wounds in hyperglycemic animals treated with chitosan-alginate membranes (CAM) produced in the presence of glycerol. 8-week C57B1 male mice were divided into normoglycemic animals with a 0.9% saline solution topical treatment (CTSF); hyperglycemic animals with 0.9% saline solution topical treatment (DMSF) and hyperglycemic animals with glycerol-plasticized chitosan-alginate membrane topical treatment (DMCAM). On post-wound day three, the DMCAM group presented a lower number of leukocytes, mature mastocytes, a higher number of vessels (<i>p</i> < 0.05), and active mastocytes (<i>p</i> < 0.05) when compared to the CTSF and DMSF groups. There were no differences regarding the distribution, deposition, organization, and thickness of collagen fibers. On day 7 there were no differences in the analysis of fibroblasts, mastocytes, and TGF−β1 and VEGF expressions among the groups. Regarding collagen fibers, the DMCAM group presented slight red-orange birefringence when compared to the CTSF and DMSF groups. On day 14 there was a slight concentration of thinner elastic fibers for the DMCAM group, with a greater reorganization of papillary skin and improved red-orange birefringence collagen fibers, as well as net-shaped orientation, similar to intact skin. In addition, improved elastic fiber organization distributed in the entire neo-dermis and a larger presence of elaunin fibers were observed, in a similar pattern found in the intact skin. The use of CAM in cutaneous lesions boosted tissue repair since there was a smaller number of inflammatory cells and mastocytes, and an improvement in collagen deposition and collagen fibers. These results demonstrate the high potential of plasticized chitosan-alginate membrane for skin wound dressing of hyperglycemic patients. |
| first_indexed | 2024-03-09T18:43:32Z |
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| id | doaj.art-d3efc842c6714a68b939dbfdd1a3f784 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T18:43:32Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
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| series | Polymers |
| spelling | doaj.art-d3efc842c6714a68b939dbfdd1a3f7842023-11-24T06:31:23ZengMDPI AGPolymers2073-43602022-11-011421475410.3390/polym14214754Histological and Molecular Evidence of the Positive Performance of Glycerol-Plasticized Chitosan-Alginate Membranes on Skin Lesions of Hyperglycemic MiceFlavia Figueiredo Azevedo0Thiago Anselmo Cantarutti1Paula de Freitas Rosa Remiro2Beatriz Barbieri3Rafael Abboud Azoubel4Mariana Harue Taniguchi Nagahara5Ângela Maria Moraes6Maria Helena Melo Lima7School of Nursing, University of Campinas, Campinas 13083-887, SP, BrazilSchool of Nursing, University of Campinas, Campinas 13083-887, SP, BrazilDepartment of Engineering of Materials and of Bioprocess, School of Chemical Engineering, University of Campinas, Campinas 13083-852, SP, BrazilSchool of Nursing, University of Campinas, Campinas 13083-887, SP, BrazilDepartment of Engineering of Materials and of Bioprocess, School of Chemical Engineering, University of Campinas, Campinas 13083-852, SP, BrazilDepartment of Engineering of Materials and of Bioprocess, School of Chemical Engineering, University of Campinas, Campinas 13083-852, SP, BrazilDepartment of Engineering of Materials and of Bioprocess, School of Chemical Engineering, University of Campinas, Campinas 13083-852, SP, BrazilSchool of Nursing, University of Campinas, Campinas 13083-887, SP, BrazilThe purpose of this study was to investigate tissue repair of excisional wounds in hyperglycemic animals treated with chitosan-alginate membranes (CAM) produced in the presence of glycerol. 8-week C57B1 male mice were divided into normoglycemic animals with a 0.9% saline solution topical treatment (CTSF); hyperglycemic animals with 0.9% saline solution topical treatment (DMSF) and hyperglycemic animals with glycerol-plasticized chitosan-alginate membrane topical treatment (DMCAM). On post-wound day three, the DMCAM group presented a lower number of leukocytes, mature mastocytes, a higher number of vessels (<i>p</i> < 0.05), and active mastocytes (<i>p</i> < 0.05) when compared to the CTSF and DMSF groups. There were no differences regarding the distribution, deposition, organization, and thickness of collagen fibers. On day 7 there were no differences in the analysis of fibroblasts, mastocytes, and TGF−β1 and VEGF expressions among the groups. Regarding collagen fibers, the DMCAM group presented slight red-orange birefringence when compared to the CTSF and DMSF groups. On day 14 there was a slight concentration of thinner elastic fibers for the DMCAM group, with a greater reorganization of papillary skin and improved red-orange birefringence collagen fibers, as well as net-shaped orientation, similar to intact skin. In addition, improved elastic fiber organization distributed in the entire neo-dermis and a larger presence of elaunin fibers were observed, in a similar pattern found in the intact skin. The use of CAM in cutaneous lesions boosted tissue repair since there was a smaller number of inflammatory cells and mastocytes, and an improvement in collagen deposition and collagen fibers. These results demonstrate the high potential of plasticized chitosan-alginate membrane for skin wound dressing of hyperglycemic patients.https://www.mdpi.com/2073-4360/14/21/4754chitosanalginatewound healingbrown adipose tissueDiabetes Mellitus |
| spellingShingle | Flavia Figueiredo Azevedo Thiago Anselmo Cantarutti Paula de Freitas Rosa Remiro Beatriz Barbieri Rafael Abboud Azoubel Mariana Harue Taniguchi Nagahara Ângela Maria Moraes Maria Helena Melo Lima Histological and Molecular Evidence of the Positive Performance of Glycerol-Plasticized Chitosan-Alginate Membranes on Skin Lesions of Hyperglycemic Mice Polymers chitosan alginate wound healing brown adipose tissue Diabetes Mellitus |
| title | Histological and Molecular Evidence of the Positive Performance of Glycerol-Plasticized Chitosan-Alginate Membranes on Skin Lesions of Hyperglycemic Mice |
| title_full | Histological and Molecular Evidence of the Positive Performance of Glycerol-Plasticized Chitosan-Alginate Membranes on Skin Lesions of Hyperglycemic Mice |
| title_fullStr | Histological and Molecular Evidence of the Positive Performance of Glycerol-Plasticized Chitosan-Alginate Membranes on Skin Lesions of Hyperglycemic Mice |
| title_full_unstemmed | Histological and Molecular Evidence of the Positive Performance of Glycerol-Plasticized Chitosan-Alginate Membranes on Skin Lesions of Hyperglycemic Mice |
| title_short | Histological and Molecular Evidence of the Positive Performance of Glycerol-Plasticized Chitosan-Alginate Membranes on Skin Lesions of Hyperglycemic Mice |
| title_sort | histological and molecular evidence of the positive performance of glycerol plasticized chitosan alginate membranes on skin lesions of hyperglycemic mice |
| topic | chitosan alginate wound healing brown adipose tissue Diabetes Mellitus |
| url | https://www.mdpi.com/2073-4360/14/21/4754 |
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