Investigating Biofilm Production, Coagulase and Hemolytic Activity in Candida Species Isolated From Denture Stomatitis Patients
Objective: Oral candidiasis, in the form of Candida-associated denture stomatitis, represents a common disease in a large percentage of denture wearers, and Candida albicans remains the most commonly isolated species. In this study, we aimed to evaluate biofilm production, coagulase and hemolytic ac...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
AVES
2011-04-01
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| Series: | Eurasian Journal of Medicine |
| Subjects: | |
| Online Access: | http://www.eajm.org/text.php3?id=380 |
| _version_ | 1797911082639032320 |
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| author | Nimet Yigit Esin Aktas Saadettin Dagistan Ahmet Ayyildiz |
| author_facet | Nimet Yigit Esin Aktas Saadettin Dagistan Ahmet Ayyildiz |
| author_sort | Nimet Yigit |
| collection | DOAJ |
| description | Objective: Oral candidiasis, in the form of Candida-associated denture stomatitis, represents a common disease in a large percentage of denture wearers, and Candida albicans remains the most commonly isolated species. In this study, we aimed to evaluate biofilm production, coagulase and hemolytic activity of Candida species isolated from denture stomatitis patients. Materials and Methods: This study included 70 patients (31 female, 39 male). Forty-eight of the patients were found to have a positive culture. A total of 48 Candida isolates representing five species, C. albicans (n=17), C. glabrata (n=10), C. krusei (n=9), C. kefyr (n=7) and C. parapsilosis (n=5), were tested. Their coagulase activities were evaluated by a classical tube coagulase test with rabbit plasma. A blood plate assay on 3% enriched sheep blood Sabouraud-dextrose agar (SDA) was used to determine their in vitro hemolytic activities. Biofilm production was determined by a visual tube method. Results: Twenty-one Candida isolates exhibited coagulase activity, and the coagulase activities of the C. albicans (64.7%) isolates were higher than other species. C. albicans, C. glabrata, C. kefyr and C. krusei species demonstrated beta hemolysis. C. parapsilosis strains failed to demonstrate any hemolytic activities. Fifteen (88.0%) of the C. albicans strains were biofilm positive. Six (35.2%) of these strains were strongly positive, 8 (47.0%) C. albicans strains were moderately positive and 1 (5.8%) C. albicans strain was weakly positive. Sixteen (51.6%) of the non-albicans Candida strains were biofilm positive while 15 (48.3%) did not produce biofilms. Conclusion: The results of this present study indicate coagulase, hemolytic activity and biofilm production by Candida spp. isolated from patients with denture stomatitis. Investigations of these virulence factors might be helpful in gaining information about the possible virulence of oral Candida species related to denture stomatitis. |
| first_indexed | 2024-04-10T11:34:40Z |
| format | Article |
| id | doaj.art-d3f1310aa9a54f7ebdf39a30aa196298 |
| institution | Directory Open Access Journal |
| issn | 1308-8734 1308-8742 |
| language | English |
| last_indexed | 2024-04-10T11:34:40Z |
| publishDate | 2011-04-01 |
| publisher | AVES |
| record_format | Article |
| series | Eurasian Journal of Medicine |
| spelling | doaj.art-d3f1310aa9a54f7ebdf39a30aa1962982023-02-15T16:17:55ZengAVESEurasian Journal of Medicine1308-87341308-87422011-04-0143012732Investigating Biofilm Production, Coagulase and Hemolytic Activity in Candida Species Isolated From Denture Stomatitis PatientsNimet YigitEsin AktasSaadettin DagistanAhmet AyyildizObjective: Oral candidiasis, in the form of Candida-associated denture stomatitis, represents a common disease in a large percentage of denture wearers, and Candida albicans remains the most commonly isolated species. In this study, we aimed to evaluate biofilm production, coagulase and hemolytic activity of Candida species isolated from denture stomatitis patients. Materials and Methods: This study included 70 patients (31 female, 39 male). Forty-eight of the patients were found to have a positive culture. A total of 48 Candida isolates representing five species, C. albicans (n=17), C. glabrata (n=10), C. krusei (n=9), C. kefyr (n=7) and C. parapsilosis (n=5), were tested. Their coagulase activities were evaluated by a classical tube coagulase test with rabbit plasma. A blood plate assay on 3% enriched sheep blood Sabouraud-dextrose agar (SDA) was used to determine their in vitro hemolytic activities. Biofilm production was determined by a visual tube method. Results: Twenty-one Candida isolates exhibited coagulase activity, and the coagulase activities of the C. albicans (64.7%) isolates were higher than other species. C. albicans, C. glabrata, C. kefyr and C. krusei species demonstrated beta hemolysis. C. parapsilosis strains failed to demonstrate any hemolytic activities. Fifteen (88.0%) of the C. albicans strains were biofilm positive. Six (35.2%) of these strains were strongly positive, 8 (47.0%) C. albicans strains were moderately positive and 1 (5.8%) C. albicans strain was weakly positive. Sixteen (51.6%) of the non-albicans Candida strains were biofilm positive while 15 (48.3%) did not produce biofilms. Conclusion: The results of this present study indicate coagulase, hemolytic activity and biofilm production by Candida spp. isolated from patients with denture stomatitis. Investigations of these virulence factors might be helpful in gaining information about the possible virulence of oral Candida species related to denture stomatitis.http://www.eajm.org/text.php3?id=380Candida speciesCoagulase activityDenture stomatitis |
| spellingShingle | Nimet Yigit Esin Aktas Saadettin Dagistan Ahmet Ayyildiz Investigating Biofilm Production, Coagulase and Hemolytic Activity in Candida Species Isolated From Denture Stomatitis Patients Eurasian Journal of Medicine Candida species Coagulase activity Denture stomatitis |
| title | Investigating Biofilm Production, Coagulase and Hemolytic Activity in Candida Species Isolated From Denture Stomatitis Patients |
| title_full | Investigating Biofilm Production, Coagulase and Hemolytic Activity in Candida Species Isolated From Denture Stomatitis Patients |
| title_fullStr | Investigating Biofilm Production, Coagulase and Hemolytic Activity in Candida Species Isolated From Denture Stomatitis Patients |
| title_full_unstemmed | Investigating Biofilm Production, Coagulase and Hemolytic Activity in Candida Species Isolated From Denture Stomatitis Patients |
| title_short | Investigating Biofilm Production, Coagulase and Hemolytic Activity in Candida Species Isolated From Denture Stomatitis Patients |
| title_sort | investigating biofilm production coagulase and hemolytic activity in candida species isolated from denture stomatitis patients |
| topic | Candida species Coagulase activity Denture stomatitis |
| url | http://www.eajm.org/text.php3?id=380 |
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