Comparison of the effect of betanin on STAT3, STAT5, and KAP1 proteins in HepG2 and THLE-2 cells
Background. Several studies suggest that the pleiotropic properties of betanin may interfere with different signaling pathways. Our previous studies on human hepatocytes showed that betanin activated the nuclear factor erythroid-2-related factor 2 (Nrf2) signaling pathway. To further understand the...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Poznan University of Medical Sciences
2023-06-01
|
| Series: | Journal of Medical Science |
| Subjects: | |
| Online Access: | https://jms.ump.edu.pl/index.php/JMS/article/view/805 |
| _version_ | 1797792322226749440 |
|---|---|
| author | Hanna Szaefer Katarzyna Hadryś Hanna Gajewska Violetta Krajka-Kuźniak |
| author_facet | Hanna Szaefer Katarzyna Hadryś Hanna Gajewska Violetta Krajka-Kuźniak |
| author_sort | Hanna Szaefer |
| collection | DOAJ |
| description |
Background. Several studies suggest that the pleiotropic properties of betanin may interfere with different signaling pathways. Our previous studies on human hepatocytes showed that betanin activated the nuclear factor erythroid-2-related factor 2 (Nrf2) signaling pathway. To further understand the exact mechanism of action of betanin, we evaluated its effect on the levels of signal transducers and activators of transcription (STATs) and KRAB domain-associated protein 1 (KAP1) in hepatoma cells (HepG2) and normal human hepatocytes (THLE-2).
Material and methods. HepG2 and THLE-2 cells were treated with 2 or 10 µM betanin for 72 h. The levels of STAT3, STAT5a, STAT5b, and KAP1 proteins in cytosolic and nuclear fractions were assessed by Western blot.
Results. At a concentration of 10 μM, betanin significantly decreased the levels of STAT3, STAT5a, and STAT5b proteins in the nuclear fraction of HepG2 cells. On the other hand, no significant changes in the levels of STAT proteins were observed in THLE-2 cells. In HepG2 cells, betanin at both tested doses increased the level of KAP1. In contrast, in THLE-2 cells, betanin at a dose of 10 µM decreased the nuclear level of KAP1.
Conclusions. Betanin modulated the levels of STAT3, STAT5, and KAP1 proteins, especially in hepatoma cells. Thus, it may be considered a potential therapeutic agent for the treatment of hepatoma.
|
| first_indexed | 2024-03-13T02:31:31Z |
| format | Article |
| id | doaj.art-d3f71e25b0f34016948648d466e7a5f8 |
| institution | Directory Open Access Journal |
| issn | 2353-9798 2353-9801 |
| language | English |
| last_indexed | 2024-03-13T02:31:31Z |
| publishDate | 2023-06-01 |
| publisher | Poznan University of Medical Sciences |
| record_format | Article |
| series | Journal of Medical Science |
| spelling | doaj.art-d3f71e25b0f34016948648d466e7a5f82023-06-29T14:22:36ZengPoznan University of Medical SciencesJournal of Medical Science2353-97982353-98012023-06-0192210.20883/medical.e805Comparison of the effect of betanin on STAT3, STAT5, and KAP1 proteins in HepG2 and THLE-2 cellsHanna Szaefer0Katarzyna Hadryś1Hanna Gajewska2Violetta Krajka-Kuźniak3Department of Pharmaceutical Biochemistry, Poznan University of Medical Sciences, PolandDepartment of Pharmaceutical Biochemistry, Poznan University of Medical Sciences, PolandDepartment of Pharmaceutical Biochemistry, Poznan University of Medical Sciences, PolandDepartment of Pharmaceutical Biochemistry, Poznan University of Medical Sciences, Poland Background. Several studies suggest that the pleiotropic properties of betanin may interfere with different signaling pathways. Our previous studies on human hepatocytes showed that betanin activated the nuclear factor erythroid-2-related factor 2 (Nrf2) signaling pathway. To further understand the exact mechanism of action of betanin, we evaluated its effect on the levels of signal transducers and activators of transcription (STATs) and KRAB domain-associated protein 1 (KAP1) in hepatoma cells (HepG2) and normal human hepatocytes (THLE-2). Material and methods. HepG2 and THLE-2 cells were treated with 2 or 10 µM betanin for 72 h. The levels of STAT3, STAT5a, STAT5b, and KAP1 proteins in cytosolic and nuclear fractions were assessed by Western blot. Results. At a concentration of 10 μM, betanin significantly decreased the levels of STAT3, STAT5a, and STAT5b proteins in the nuclear fraction of HepG2 cells. On the other hand, no significant changes in the levels of STAT proteins were observed in THLE-2 cells. In HepG2 cells, betanin at both tested doses increased the level of KAP1. In contrast, in THLE-2 cells, betanin at a dose of 10 µM decreased the nuclear level of KAP1. Conclusions. Betanin modulated the levels of STAT3, STAT5, and KAP1 proteins, especially in hepatoma cells. Thus, it may be considered a potential therapeutic agent for the treatment of hepatoma. https://jms.ump.edu.pl/index.php/JMS/article/view/805BetaninSTAT3STAT5KAP1HepG2 cellsTHLE-2 cells |
| spellingShingle | Hanna Szaefer Katarzyna Hadryś Hanna Gajewska Violetta Krajka-Kuźniak Comparison of the effect of betanin on STAT3, STAT5, and KAP1 proteins in HepG2 and THLE-2 cells Journal of Medical Science Betanin STAT3 STAT5 KAP1 HepG2 cells THLE-2 cells |
| title | Comparison of the effect of betanin on STAT3, STAT5, and KAP1 proteins in HepG2 and THLE-2 cells |
| title_full | Comparison of the effect of betanin on STAT3, STAT5, and KAP1 proteins in HepG2 and THLE-2 cells |
| title_fullStr | Comparison of the effect of betanin on STAT3, STAT5, and KAP1 proteins in HepG2 and THLE-2 cells |
| title_full_unstemmed | Comparison of the effect of betanin on STAT3, STAT5, and KAP1 proteins in HepG2 and THLE-2 cells |
| title_short | Comparison of the effect of betanin on STAT3, STAT5, and KAP1 proteins in HepG2 and THLE-2 cells |
| title_sort | comparison of the effect of betanin on stat3 stat5 and kap1 proteins in hepg2 and thle 2 cells |
| topic | Betanin STAT3 STAT5 KAP1 HepG2 cells THLE-2 cells |
| url | https://jms.ump.edu.pl/index.php/JMS/article/view/805 |
| work_keys_str_mv | AT hannaszaefer comparisonoftheeffectofbetaninonstat3stat5andkap1proteinsinhepg2andthle2cells AT katarzynahadrys comparisonoftheeffectofbetaninonstat3stat5andkap1proteinsinhepg2andthle2cells AT hannagajewska comparisonoftheeffectofbetaninonstat3stat5andkap1proteinsinhepg2andthle2cells AT violettakrajkakuzniak comparisonoftheeffectofbetaninonstat3stat5andkap1proteinsinhepg2andthle2cells |