Molecular Markers for Detecting Inflorescence Size of <i>Brassica oleracea</i> L. Crops and <i>B. oleracea</i> Complex Species (n = 9) Useful for Breeding of Broccoli (<i>B. oleracea</i> var. <i>italica</i>) and Cauliflower (<i>B. oleracea</i> var. <i>botrytis</i>)
The gene flow from <i>Brassica oleracea</i> L. wild relatives to <i>B. oleracea</i> vegetable crops have occurred and continue to occur ordinarily in several Mediterranean countries, such as Sicily, representing an important hot spot of diversity for some of them, such as bro...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-01-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/12/2/407 |
| _version_ | 1797437599961317376 |
|---|---|
| author | Simone Treccarichi Hajer Ben Ammar Marwen Amari Riccardo Cali Alessandro Tribulato Ferdinando Branca |
| author_facet | Simone Treccarichi Hajer Ben Ammar Marwen Amari Riccardo Cali Alessandro Tribulato Ferdinando Branca |
| author_sort | Simone Treccarichi |
| collection | DOAJ |
| description | The gene flow from <i>Brassica oleracea</i> L. wild relatives to <i>B. oleracea</i> vegetable crops have occurred and continue to occur ordinarily in several Mediterranean countries, such as Sicily, representing an important hot spot of diversity for some of them, such as broccoli, cauliflower and kale. For detecting and for exploiting the forgotten alleles lost during the domestication processes of the <i>B. oleracea</i> crops, attention has been pointed to the individuation of specific markers for individuating genotypes characterized by hypertrophic inflorescence traits by the marker assisted selection (MAS) during the first plant growing phases after the crosses between broccoli (<i>B. oleracea</i> var. <i>italica</i>)/cauliflower (<i>B. oleracea</i> var. <i>botrytis</i>) with <i>B. oleracea</i> wild relatives (n = 9), reducing the cultivation and evaluation costs. The desired traits often found in several <i>B. oleracea</i> wild relatives are mainly addressed to improve the plant resistance to biotic and abiotic stresses and to increase the organoleptic, nutritive and nutraceutical traits of the products. One of the targeted traits for broccoli and cauliflower breeding is represented by the inflorescences size as is documented by the domestication processes of these two crops. Based on the previous results achieved, the numerical matrix, obtained utilizing five simple sequence repeats (SSRs), was analyzed to assess the relationship among the main inflorescence characteristics and the allelic variation of the SSRs loci analyzed (BoABI1, BoAP1, BoPLD1, BoTHL1 and PBCGSSRBo39), both for the <i>Brassica oleracea</i> and <i>B</i>. <i>oleracea</i> wild relatives (n = 9) accessions set. The main inflorescence morphometric characteristics, such as weight, height, diameter, shape, inflorescence curvature angle and its stem diameter, were registered before the flower anthesis. We analyzed the correlations among the allelic variation of the SSRs primers utilized and the inflorescence morphometric characteristics to individuate genomic regions stimulating the hypertrophy of the reproductive organ. The relationships found explain the diversity among <i>B. oleracea</i> crops and the <i>B. oleracea</i> complex species (n = 9) for the inflorescence size and structure. The individuated markers allow important time reduction during the breeding programs after crossing wild species for transferring useful biotic and abiotic resistances and organoleptic and nutraceutical traits to the <i>B. oleracea</i> crops by MAS. |
| first_indexed | 2024-03-09T11:24:48Z |
| format | Article |
| id | doaj.art-d3f80cda7dfa4957b36b94f02a55ac3f |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T11:24:48Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-d3f80cda7dfa4957b36b94f02a55ac3f2023-12-01T00:06:24ZengMDPI AGPlants2223-77472023-01-0112240710.3390/plants12020407Molecular Markers for Detecting Inflorescence Size of <i>Brassica oleracea</i> L. Crops and <i>B. oleracea</i> Complex Species (n = 9) Useful for Breeding of Broccoli (<i>B. oleracea</i> var. <i>italica</i>) and Cauliflower (<i>B. oleracea</i> var. <i>botrytis</i>)Simone Treccarichi0Hajer Ben Ammar1Marwen Amari2Riccardo Cali3Alessandro Tribulato4Ferdinando Branca5Department of Agriculture, Food and Environment (Di3A), University of Catania, 95131 Catania, ItalyDepartment of Agriculture, Food and Environment (Di3A), University of Catania, 95131 Catania, ItalyDepartment of Agriculture, Food and Environment (Di3A), University of Catania, 95131 Catania, ItalyDepartment of Agriculture, Food and Environment (Di3A), University of Catania, 95131 Catania, ItalyDepartment of Agriculture, Food and Environment (Di3A), University of Catania, 95131 Catania, ItalyDepartment of Agriculture, Food and Environment (Di3A), University of Catania, 95131 Catania, ItalyThe gene flow from <i>Brassica oleracea</i> L. wild relatives to <i>B. oleracea</i> vegetable crops have occurred and continue to occur ordinarily in several Mediterranean countries, such as Sicily, representing an important hot spot of diversity for some of them, such as broccoli, cauliflower and kale. For detecting and for exploiting the forgotten alleles lost during the domestication processes of the <i>B. oleracea</i> crops, attention has been pointed to the individuation of specific markers for individuating genotypes characterized by hypertrophic inflorescence traits by the marker assisted selection (MAS) during the first plant growing phases after the crosses between broccoli (<i>B. oleracea</i> var. <i>italica</i>)/cauliflower (<i>B. oleracea</i> var. <i>botrytis</i>) with <i>B. oleracea</i> wild relatives (n = 9), reducing the cultivation and evaluation costs. The desired traits often found in several <i>B. oleracea</i> wild relatives are mainly addressed to improve the plant resistance to biotic and abiotic stresses and to increase the organoleptic, nutritive and nutraceutical traits of the products. One of the targeted traits for broccoli and cauliflower breeding is represented by the inflorescences size as is documented by the domestication processes of these two crops. Based on the previous results achieved, the numerical matrix, obtained utilizing five simple sequence repeats (SSRs), was analyzed to assess the relationship among the main inflorescence characteristics and the allelic variation of the SSRs loci analyzed (BoABI1, BoAP1, BoPLD1, BoTHL1 and PBCGSSRBo39), both for the <i>Brassica oleracea</i> and <i>B</i>. <i>oleracea</i> wild relatives (n = 9) accessions set. The main inflorescence morphometric characteristics, such as weight, height, diameter, shape, inflorescence curvature angle and its stem diameter, were registered before the flower anthesis. We analyzed the correlations among the allelic variation of the SSRs primers utilized and the inflorescence morphometric characteristics to individuate genomic regions stimulating the hypertrophy of the reproductive organ. The relationships found explain the diversity among <i>B. oleracea</i> crops and the <i>B. oleracea</i> complex species (n = 9) for the inflorescence size and structure. The individuated markers allow important time reduction during the breeding programs after crossing wild species for transferring useful biotic and abiotic resistances and organoleptic and nutraceutical traits to the <i>B. oleracea</i> crops by MAS.https://www.mdpi.com/2223-7747/12/2/407<i>Brassica</i> complex species (n = 9)MADH–box genesSSRs assayheterozygosity indexallelic varianceinflorescence morphometric traits |
| spellingShingle | Simone Treccarichi Hajer Ben Ammar Marwen Amari Riccardo Cali Alessandro Tribulato Ferdinando Branca Molecular Markers for Detecting Inflorescence Size of <i>Brassica oleracea</i> L. Crops and <i>B. oleracea</i> Complex Species (n = 9) Useful for Breeding of Broccoli (<i>B. oleracea</i> var. <i>italica</i>) and Cauliflower (<i>B. oleracea</i> var. <i>botrytis</i>) Plants <i>Brassica</i> complex species (n = 9) MADH–box genes SSRs assay heterozygosity index allelic variance inflorescence morphometric traits |
| title | Molecular Markers for Detecting Inflorescence Size of <i>Brassica oleracea</i> L. Crops and <i>B. oleracea</i> Complex Species (n = 9) Useful for Breeding of Broccoli (<i>B. oleracea</i> var. <i>italica</i>) and Cauliflower (<i>B. oleracea</i> var. <i>botrytis</i>) |
| title_full | Molecular Markers for Detecting Inflorescence Size of <i>Brassica oleracea</i> L. Crops and <i>B. oleracea</i> Complex Species (n = 9) Useful for Breeding of Broccoli (<i>B. oleracea</i> var. <i>italica</i>) and Cauliflower (<i>B. oleracea</i> var. <i>botrytis</i>) |
| title_fullStr | Molecular Markers for Detecting Inflorescence Size of <i>Brassica oleracea</i> L. Crops and <i>B. oleracea</i> Complex Species (n = 9) Useful for Breeding of Broccoli (<i>B. oleracea</i> var. <i>italica</i>) and Cauliflower (<i>B. oleracea</i> var. <i>botrytis</i>) |
| title_full_unstemmed | Molecular Markers for Detecting Inflorescence Size of <i>Brassica oleracea</i> L. Crops and <i>B. oleracea</i> Complex Species (n = 9) Useful for Breeding of Broccoli (<i>B. oleracea</i> var. <i>italica</i>) and Cauliflower (<i>B. oleracea</i> var. <i>botrytis</i>) |
| title_short | Molecular Markers for Detecting Inflorescence Size of <i>Brassica oleracea</i> L. Crops and <i>B. oleracea</i> Complex Species (n = 9) Useful for Breeding of Broccoli (<i>B. oleracea</i> var. <i>italica</i>) and Cauliflower (<i>B. oleracea</i> var. <i>botrytis</i>) |
| title_sort | molecular markers for detecting inflorescence size of i brassica oleracea i l crops and i b oleracea i complex species n 9 useful for breeding of broccoli i b oleracea i var i italica i and cauliflower i b oleracea i var i botrytis i |
| topic | <i>Brassica</i> complex species (n = 9) MADH–box genes SSRs assay heterozygosity index allelic variance inflorescence morphometric traits |
| url | https://www.mdpi.com/2223-7747/12/2/407 |
| work_keys_str_mv | AT simonetreccarichi molecularmarkersfordetectinginflorescencesizeofibrassicaoleraceailcropsandiboleraceaicomplexspeciesn9usefulforbreedingofbroccoliiboleraceaivariitalicaiandcaulifloweriboleraceaivaribotrytisi AT hajerbenammar molecularmarkersfordetectinginflorescencesizeofibrassicaoleraceailcropsandiboleraceaicomplexspeciesn9usefulforbreedingofbroccoliiboleraceaivariitalicaiandcaulifloweriboleraceaivaribotrytisi AT marwenamari molecularmarkersfordetectinginflorescencesizeofibrassicaoleraceailcropsandiboleraceaicomplexspeciesn9usefulforbreedingofbroccoliiboleraceaivariitalicaiandcaulifloweriboleraceaivaribotrytisi AT riccardocali molecularmarkersfordetectinginflorescencesizeofibrassicaoleraceailcropsandiboleraceaicomplexspeciesn9usefulforbreedingofbroccoliiboleraceaivariitalicaiandcaulifloweriboleraceaivaribotrytisi AT alessandrotribulato molecularmarkersfordetectinginflorescencesizeofibrassicaoleraceailcropsandiboleraceaicomplexspeciesn9usefulforbreedingofbroccoliiboleraceaivariitalicaiandcaulifloweriboleraceaivaribotrytisi AT ferdinandobranca molecularmarkersfordetectinginflorescencesizeofibrassicaoleraceailcropsandiboleraceaicomplexspeciesn9usefulforbreedingofbroccoliiboleraceaivariitalicaiandcaulifloweriboleraceaivaribotrytisi |