Prevalence of Listeria Species in Raw Milk in Isfahan, Iran
Background: Listeria monocytogenes is an important psychrotrophic foodborne pathogen which may exist in milk and dairy products. The bacterium causes listeriosis with severe clinical consequences such as meningitis, septicemia and abortion. Therefore, contamination of food stuff implies a significan...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | fas |
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Isfahan University of Medical Sciences
2012-11-01
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| Series: | مجله دانشکده پزشکی اصفهان |
| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/2121 |
| _version_ | 1797756870199345152 |
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| author | Ehsan Shamloo Aghakhani Mohammad Jalali Maryam Mirlohi Zohre Abdi Moghadam Elham Shamloo Aghakhani Mohammad Reza Maracy Majid Yaran |
| author_facet | Ehsan Shamloo Aghakhani Mohammad Jalali Maryam Mirlohi Zohre Abdi Moghadam Elham Shamloo Aghakhani Mohammad Reza Maracy Majid Yaran |
| author_sort | Ehsan Shamloo Aghakhani |
| collection | DOAJ |
| description | Background: Listeria monocytogenes is an important psychrotrophic foodborne pathogen which may exist in milk and dairy products. The bacterium causes listeriosis with severe clinical consequences such as meningitis, septicemia and abortion. Therefore, contamination of food stuff implies a significant health risk for human. There are a minimum data on raw milk contamination of Listeria species In Iran. Thus, the objective of this study was to assess the prevalence of Listeria spp. in raw milk in Isfahan, Iran.
Methods: A total of 91 samples of raw milk were examined for the presence of Listeria spp. using a two-step selective enrichment recommended by USDA. All isolates were subjected to standard biochemical test. L. monocytogenes strains were further confirmed by PCR amplification.
Findings: Of the 91 raw milk samples collected, Listeria species were isolated on five (5.49%) samples. Four (4.39%) of these five isolates identified as L. monocytogenes and one (1.09%) as L. seeligeri. All strains of L. monocytogenes identified by biochemical tests, were also confirmed by PCR.
Conclusion: The study shows the prevalence of L. monocytogenes in raw milk sold in the market. Consumption of raw milk with mild heat treatment or its usage in traditional dishes is a common practice in Iran. Therefore, the lack of appropriate control measures could pose serious health problems. The lack of knowledge on the risks of listeriosis transmission indicating the need of implementation of food safety education program. In addition, the Iranian food safety authorities urgently should set up an effective standard to screen all susceptible food for the presence of Listeria.
Keywords: Listeria, Raw milk, Prevalence, PCR |
| first_indexed | 2024-03-12T18:07:47Z |
| format | Article |
| id | doaj.art-d3fb968c01194b8bafb18707acf9c317 |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T18:07:47Z |
| publishDate | 2012-11-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-d3fb968c01194b8bafb18707acf9c3172023-08-02T09:28:08ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2012-11-01302041141Prevalence of Listeria Species in Raw Milk in Isfahan, IranEhsan Shamloo Aghakhani0Mohammad Jalali1Maryam Mirlohi2Zohre Abdi Moghadam3Elham Shamloo Aghakhani4Mohammad Reza Maracy5Majid Yaran6MSc Student, Department of Food Science and Technology, School of Nutrition and Food Sciences AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, IranAssociate Professor, Department of Food Science and Technology, School of Nutrition and Food Sciences, Isfahan University of Medical Sciences, Isfahan, IranAssistant Professor, Department of Food Science and Technology, School of Nutrition and Food Sciences, Isfahan University of Medical Sciences, Isfahan, IranMSc Student, Department of Food Science and Technology, School of Nutrition and Food Sciences AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, IranMSc Student, Department of Food and Drug Control, School of Pharmacy, Tehran University of Medical Sciences, Tehran, IranAssociate Professor, Department of Epidemiology, School of Health, Isfahan University of Medical Sciences, Isfahan, IranInfectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, IranBackground: Listeria monocytogenes is an important psychrotrophic foodborne pathogen which may exist in milk and dairy products. The bacterium causes listeriosis with severe clinical consequences such as meningitis, septicemia and abortion. Therefore, contamination of food stuff implies a significant health risk for human. There are a minimum data on raw milk contamination of Listeria species In Iran. Thus, the objective of this study was to assess the prevalence of Listeria spp. in raw milk in Isfahan, Iran. Methods: A total of 91 samples of raw milk were examined for the presence of Listeria spp. using a two-step selective enrichment recommended by USDA. All isolates were subjected to standard biochemical test. L. monocytogenes strains were further confirmed by PCR amplification. Findings: Of the 91 raw milk samples collected, Listeria species were isolated on five (5.49%) samples. Four (4.39%) of these five isolates identified as L. monocytogenes and one (1.09%) as L. seeligeri. All strains of L. monocytogenes identified by biochemical tests, were also confirmed by PCR. Conclusion: The study shows the prevalence of L. monocytogenes in raw milk sold in the market. Consumption of raw milk with mild heat treatment or its usage in traditional dishes is a common practice in Iran. Therefore, the lack of appropriate control measures could pose serious health problems. The lack of knowledge on the risks of listeriosis transmission indicating the need of implementation of food safety education program. In addition, the Iranian food safety authorities urgently should set up an effective standard to screen all susceptible food for the presence of Listeria. Keywords: Listeria, Raw milk, Prevalence, PCRhttp://jims.mui.ac.ir/index.php/jims/article/view/2121 |
| spellingShingle | Ehsan Shamloo Aghakhani Mohammad Jalali Maryam Mirlohi Zohre Abdi Moghadam Elham Shamloo Aghakhani Mohammad Reza Maracy Majid Yaran Prevalence of Listeria Species in Raw Milk in Isfahan, Iran مجله دانشکده پزشکی اصفهان |
| title | Prevalence of Listeria Species in Raw Milk in Isfahan, Iran |
| title_full | Prevalence of Listeria Species in Raw Milk in Isfahan, Iran |
| title_fullStr | Prevalence of Listeria Species in Raw Milk in Isfahan, Iran |
| title_full_unstemmed | Prevalence of Listeria Species in Raw Milk in Isfahan, Iran |
| title_short | Prevalence of Listeria Species in Raw Milk in Isfahan, Iran |
| title_sort | prevalence of listeria species in raw milk in isfahan iran |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/2121 |
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