Antifungal activity and mechanism of action of monoterpenes against Botrytis cinerea
ABSTRACT Botrytis cinerea is considered one of the most important post-harvest pathogens being the causative agent of gray rot. To reduce the use of synthetic fungicides, it is important to explore alternative products with antifungal properties. Among these alternative products are essential oils,...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Lavras
2024-04-01
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| Series: | Ciência e Agrotecnologia |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542024000100201&lng=en&tlng=en |
| _version_ | 1797205769677963264 |
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| author | Marília Brandão Pedroso Fernando Joel Scariot Ronaldo Kaue Mattos Rocha Sergio Echeverrigaray Ana Paula Longaray Delamare |
| author_facet | Marília Brandão Pedroso Fernando Joel Scariot Ronaldo Kaue Mattos Rocha Sergio Echeverrigaray Ana Paula Longaray Delamare |
| author_sort | Marília Brandão Pedroso |
| collection | DOAJ |
| description | ABSTRACT Botrytis cinerea is considered one of the most important post-harvest pathogens being the causative agent of gray rot. To reduce the use of synthetic fungicides, it is important to explore alternative products with antifungal properties. Among these alternative products are essential oils, which present monoterpenes as major compounds. The objective of this study was to evaluate the effects of eight monoterpenes (1,8-cineole, carvacrol, citral, citronellal, citronellol, geraniol, linalool, and thymol) on the control of B. cinerea. The mycelial growth of B. cinerea was assessed after treating it with the monoterpenes at a concentration of 1,000 mg/L. Subsequently, the minimal inhibitory concentrations (IC90) of the monoterpenes that showed the greatest antifungal potential were determined. Carvacrol and thymol were tested on B. cinerea cell membrane integrity, intracellular accumulation of reactive oxygen species (ROS), and mitochondrial membrane potential of the conidia. Among the tested monoterpenes carvacrol, citral, citronellal, citronellol, geraniol, and thymol demonstrated complete inhibition of mycelial growth at a concentration of 1,000 mg/L. Carvacrol and thymol exhibited the lowest IC90 values against B. cinerea, with an IC90 of 125 mg/L. Furthermore, carvacrol and thymol induced conidial death in a dose-dependent manner, resulting in the disruption of cell membrane integrity, increased intracellular ROS levels, and decreased mitochondrial membrane potential. These findings highlight the potential of carvacrol and thymol as alternative means of controlling B. cinerea. |
| first_indexed | 2024-04-24T08:56:23Z |
| format | Article |
| id | doaj.art-d3fd4f2c57b9489192941ec9e3c48dcd |
| institution | Directory Open Access Journal |
| issn | 1981-1829 |
| language | English |
| last_indexed | 2024-04-24T08:56:23Z |
| publishDate | 2024-04-01 |
| publisher | Universidade Federal de Lavras |
| record_format | Article |
| series | Ciência e Agrotecnologia |
| spelling | doaj.art-d3fd4f2c57b9489192941ec9e3c48dcd2024-04-16T07:39:34ZengUniversidade Federal de LavrasCiência e Agrotecnologia1981-18292024-04-014810.1590/1413-7054202448018823Antifungal activity and mechanism of action of monoterpenes against Botrytis cinereaMarília Brandão Pedrosohttps://orcid.org/0000-0001-9149-9756Fernando Joel Scariothttps://orcid.org/0000-0003-0848-2772Ronaldo Kaue Mattos Rochahttps://orcid.org/0000-0001-5798-3755Sergio Echeverrigarayhttps://orcid.org/0000-0002-9111-0701Ana Paula Longaray Delamarehttps://orcid.org/0000-0001-9638-5483ABSTRACT Botrytis cinerea is considered one of the most important post-harvest pathogens being the causative agent of gray rot. To reduce the use of synthetic fungicides, it is important to explore alternative products with antifungal properties. Among these alternative products are essential oils, which present monoterpenes as major compounds. The objective of this study was to evaluate the effects of eight monoterpenes (1,8-cineole, carvacrol, citral, citronellal, citronellol, geraniol, linalool, and thymol) on the control of B. cinerea. The mycelial growth of B. cinerea was assessed after treating it with the monoterpenes at a concentration of 1,000 mg/L. Subsequently, the minimal inhibitory concentrations (IC90) of the monoterpenes that showed the greatest antifungal potential were determined. Carvacrol and thymol were tested on B. cinerea cell membrane integrity, intracellular accumulation of reactive oxygen species (ROS), and mitochondrial membrane potential of the conidia. Among the tested monoterpenes carvacrol, citral, citronellal, citronellol, geraniol, and thymol demonstrated complete inhibition of mycelial growth at a concentration of 1,000 mg/L. Carvacrol and thymol exhibited the lowest IC90 values against B. cinerea, with an IC90 of 125 mg/L. Furthermore, carvacrol and thymol induced conidial death in a dose-dependent manner, resulting in the disruption of cell membrane integrity, increased intracellular ROS levels, and decreased mitochondrial membrane potential. These findings highlight the potential of carvacrol and thymol as alternative means of controlling B. cinerea.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542024000100201&lng=en&tlng=enGray rotcarvacrolthymolalternative control. |
| spellingShingle | Marília Brandão Pedroso Fernando Joel Scariot Ronaldo Kaue Mattos Rocha Sergio Echeverrigaray Ana Paula Longaray Delamare Antifungal activity and mechanism of action of monoterpenes against Botrytis cinerea Ciência e Agrotecnologia Gray rot carvacrol thymol alternative control. |
| title | Antifungal activity and mechanism of action of monoterpenes against Botrytis cinerea |
| title_full | Antifungal activity and mechanism of action of monoterpenes against Botrytis cinerea |
| title_fullStr | Antifungal activity and mechanism of action of monoterpenes against Botrytis cinerea |
| title_full_unstemmed | Antifungal activity and mechanism of action of monoterpenes against Botrytis cinerea |
| title_short | Antifungal activity and mechanism of action of monoterpenes against Botrytis cinerea |
| title_sort | antifungal activity and mechanism of action of monoterpenes against botrytis cinerea |
| topic | Gray rot carvacrol thymol alternative control. |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542024000100201&lng=en&tlng=en |
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