In Vitro Assessment of the Cell Metabolic Activity, Cytotoxicity, Cell Attachment, and Inflammatory Reaction of Human Oral Fibroblasts on Polyetheretherketone (PEEK) Implant–Abutment
The purpose of this research is to compare the cytotoxicity of polyetheretherketone (PEEK) and polyetherketoneketone (PEKK) with conventional dental implant–abutment materials, namely titanium alloy (Ti-6Al-4V) and yttria-stabilized tetragonal zirconia polycrystal (Y-TZP), to evaluate the cell metab...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/13/17/2995 |
| _version_ | 1797520989535338496 |
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| author | Tzu-Yu Peng Yin-Hwa Shih Shih-Min Hsia Tong-Hong Wang Po-Jung Li Dan-Jae Lin Kuo-Ting Sun Kuo-Chou Chiu Tzong-Ming Shieh |
| author_facet | Tzu-Yu Peng Yin-Hwa Shih Shih-Min Hsia Tong-Hong Wang Po-Jung Li Dan-Jae Lin Kuo-Ting Sun Kuo-Chou Chiu Tzong-Ming Shieh |
| author_sort | Tzu-Yu Peng |
| collection | DOAJ |
| description | The purpose of this research is to compare the cytotoxicity of polyetheretherketone (PEEK) and polyetherketoneketone (PEKK) with conventional dental implant–abutment materials, namely titanium alloy (Ti-6Al-4V) and yttria-stabilized tetragonal zirconia polycrystal (Y-TZP), to evaluate the cell metabolic activity, cytotoxicity, and inflammation potential of human oral fibroblasts (HOF) on these materials. Disk-shaped specimens were designed and prepared via a dental computer-aided manufacturing technology system. Surface topography, roughness, and free energy were investigated by atomic force microscope and contact angle analyzer; cell metabolic activity and cytotoxicity by MTT assay; and morphological changes by scanning electron microscopy (SEM). The effect of pro-inflammatory gene expression was evaluated by RT-qPCR. The obtained data were analyzed with one-way analysis of variance and post-hoc Tukey’s honest significant difference tests. PEEK and PEKK exhibited higher submicron surface roughness (0.04 μm) and hydrophobicity (>80°) than the control. Although the cell activity of PEEK was lower than that of Ti-6Al-4V and Y-TZP for the first 24 h (<i>p</i> < 0.05), after 48 h there was no difference (<i>p</i> > 0.05). According to the cell cytotoxicity and the pro-inflammatory cytokine gene expression assays, there was no difference between the materials (<i>p</i> > 0.05). SEM observations indicated that HOF adhered poorly to PEKK but properly to Ti-6Al-4V, Y-TZP, and PEEK. PEEK and PEKK show comparable epithelial biological responses to Ti-6Al-4V and Y-TZP as implant–abutment materials. Between the two polymeric materials, the PEEK surface, where the HOF showed better cell metabolic activity and cytotoxicity, was a more promising implant–abutment material. |
| first_indexed | 2024-03-10T08:06:17Z |
| format | Article |
| id | doaj.art-d400529ca1d3474582cba796315ee10c |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T08:06:17Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-d400529ca1d3474582cba796315ee10c2023-11-22T11:06:29ZengMDPI AGPolymers2073-43602021-09-011317299510.3390/polym13172995In Vitro Assessment of the Cell Metabolic Activity, Cytotoxicity, Cell Attachment, and Inflammatory Reaction of Human Oral Fibroblasts on Polyetheretherketone (PEEK) Implant–AbutmentTzu-Yu Peng0Yin-Hwa Shih1Shih-Min Hsia2Tong-Hong Wang3Po-Jung Li4Dan-Jae Lin5Kuo-Ting Sun6Kuo-Chou Chiu7Tzong-Ming Shieh8School of Dentistry, College of Dentistry, China Medical University, Taichung 40402, TaiwanDepartment of Healthcare Administration, College of Medical and Health Science, Asia University, Taichung 41354, TaiwanSchool of Nutrition and Health Sciences, Taipei Medical University, Taipei 11031, TaiwanTissue Bank, Chang Gung Memorial Hospital, Taoyuan 33305, TaiwanSchool of Dentistry, College of Dentistry, China Medical University, Taichung 40402, TaiwanSchool of Dentistry, College of Dentistry, China Medical University, Taichung 40402, TaiwanSchool of Dentistry, College of Dentistry, China Medical University, Taichung 40402, TaiwanDivision of Oral Diagnosis and Family Dentistry, Tri-Service General Hospital, National Defense Medical Center, Taipei 11490, TaiwanSchool of Dentistry, College of Dentistry, China Medical University, Taichung 40402, TaiwanThe purpose of this research is to compare the cytotoxicity of polyetheretherketone (PEEK) and polyetherketoneketone (PEKK) with conventional dental implant–abutment materials, namely titanium alloy (Ti-6Al-4V) and yttria-stabilized tetragonal zirconia polycrystal (Y-TZP), to evaluate the cell metabolic activity, cytotoxicity, and inflammation potential of human oral fibroblasts (HOF) on these materials. Disk-shaped specimens were designed and prepared via a dental computer-aided manufacturing technology system. Surface topography, roughness, and free energy were investigated by atomic force microscope and contact angle analyzer; cell metabolic activity and cytotoxicity by MTT assay; and morphological changes by scanning electron microscopy (SEM). The effect of pro-inflammatory gene expression was evaluated by RT-qPCR. The obtained data were analyzed with one-way analysis of variance and post-hoc Tukey’s honest significant difference tests. PEEK and PEKK exhibited higher submicron surface roughness (0.04 μm) and hydrophobicity (>80°) than the control. Although the cell activity of PEEK was lower than that of Ti-6Al-4V and Y-TZP for the first 24 h (<i>p</i> < 0.05), after 48 h there was no difference (<i>p</i> > 0.05). According to the cell cytotoxicity and the pro-inflammatory cytokine gene expression assays, there was no difference between the materials (<i>p</i> > 0.05). SEM observations indicated that HOF adhered poorly to PEKK but properly to Ti-6Al-4V, Y-TZP, and PEEK. PEEK and PEKK show comparable epithelial biological responses to Ti-6Al-4V and Y-TZP as implant–abutment materials. Between the two polymeric materials, the PEEK surface, where the HOF showed better cell metabolic activity and cytotoxicity, was a more promising implant–abutment material.https://www.mdpi.com/2073-4360/13/17/2995polyaryletherketonepolyetheretherketonepolyetherketoneketoneCAD/CAMdigital dentistryimplant–abutment |
| spellingShingle | Tzu-Yu Peng Yin-Hwa Shih Shih-Min Hsia Tong-Hong Wang Po-Jung Li Dan-Jae Lin Kuo-Ting Sun Kuo-Chou Chiu Tzong-Ming Shieh In Vitro Assessment of the Cell Metabolic Activity, Cytotoxicity, Cell Attachment, and Inflammatory Reaction of Human Oral Fibroblasts on Polyetheretherketone (PEEK) Implant–Abutment Polymers polyaryletherketone polyetheretherketone polyetherketoneketone CAD/CAM digital dentistry implant–abutment |
| title | In Vitro Assessment of the Cell Metabolic Activity, Cytotoxicity, Cell Attachment, and Inflammatory Reaction of Human Oral Fibroblasts on Polyetheretherketone (PEEK) Implant–Abutment |
| title_full | In Vitro Assessment of the Cell Metabolic Activity, Cytotoxicity, Cell Attachment, and Inflammatory Reaction of Human Oral Fibroblasts on Polyetheretherketone (PEEK) Implant–Abutment |
| title_fullStr | In Vitro Assessment of the Cell Metabolic Activity, Cytotoxicity, Cell Attachment, and Inflammatory Reaction of Human Oral Fibroblasts on Polyetheretherketone (PEEK) Implant–Abutment |
| title_full_unstemmed | In Vitro Assessment of the Cell Metabolic Activity, Cytotoxicity, Cell Attachment, and Inflammatory Reaction of Human Oral Fibroblasts on Polyetheretherketone (PEEK) Implant–Abutment |
| title_short | In Vitro Assessment of the Cell Metabolic Activity, Cytotoxicity, Cell Attachment, and Inflammatory Reaction of Human Oral Fibroblasts on Polyetheretherketone (PEEK) Implant–Abutment |
| title_sort | in vitro assessment of the cell metabolic activity cytotoxicity cell attachment and inflammatory reaction of human oral fibroblasts on polyetheretherketone peek implant abutment |
| topic | polyaryletherketone polyetheretherketone polyetherketoneketone CAD/CAM digital dentistry implant–abutment |
| url | https://www.mdpi.com/2073-4360/13/17/2995 |
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