An Efficient cDNA-AFLP-Based Strategy for the Identification of Putative Pathogenicity Factors from the Potato Cyst Nematode Globodera rostochiensis
A new strategy has been designed to identify putative pathogenicity factors from the dorsal or subventral esophageal glands of the potato cyst nematode Globodera rostochiensis. Three independent criteria were used for selection. First, genes of interest should predominantly be expressed in infective...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
The American Phytopathological Society
2000-08-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2000.13.8.830 |
| _version_ | 1819085075800653824 |
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| author | Ling Qin Hein Overmars Johannes Helder Herman Popeijus Jeroen Rouppe van der Voort Wouter Groenink Paul van Koert Arjen Schots Jaap Bakker Geert Smant |
| author_facet | Ling Qin Hein Overmars Johannes Helder Herman Popeijus Jeroen Rouppe van der Voort Wouter Groenink Paul van Koert Arjen Schots Jaap Bakker Geert Smant |
| author_sort | Ling Qin |
| collection | DOAJ |
| description | A new strategy has been designed to identify putative pathogenicity factors from the dorsal or subventral esophageal glands of the potato cyst nematode Globodera rostochiensis. Three independent criteria were used for selection. First, genes of interest should predominantly be expressed in infective second-stage juveniles, and not, or to a far lesser extent, in younger developmental stages. For this, gene expression profiles from five different developmental stages were generated with cDNA-AFLP (amplified fragment length polymorphism). Secondly, the mRNA corresponding to such a putative pathogenicity factor should predominantly be present in the esophageal glands of pre-parasitic juveniles. This was checked by in situ hybridization. As a third criterion, these proteinaceous factors should be preceded by a signal peptide for secretion. Expression profiles of more than 4,000 genes were generated and three up-regulated, dorsal gland-specific proteins preceded by signal peptide for secretion were identified. No dorsal gland genes have been cloned before from plant-parasitic nematodes. The partial sequence of these three factors, A4, A18, and A41, showed no significant homology to any known gene. Their presence in the dorsal glands of infective juveniles suggests that these proteins could be involved in feeding cell initiation, and not in migration in the plant root or in protection against plant defense responses. Finally, the applicability of this new strategy in other plant-microbe interactions is discussed. |
| first_indexed | 2024-12-21T20:58:36Z |
| format | Article |
| id | doaj.art-d402104702b54a2fa8d80be849492d4b |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-21T20:58:36Z |
| publishDate | 2000-08-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-d402104702b54a2fa8d80be849492d4b2022-12-21T18:50:30ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062000-08-0113883083610.1094/MPMI.2000.13.8.830An Efficient cDNA-AFLP-Based Strategy for the Identification of Putative Pathogenicity Factors from the Potato Cyst Nematode Globodera rostochiensisLing QinHein OvermarsJohannes HelderHerman PopeijusJeroen Rouppe van der VoortWouter GroeninkPaul van KoertArjen SchotsJaap BakkerGeert SmantA new strategy has been designed to identify putative pathogenicity factors from the dorsal or subventral esophageal glands of the potato cyst nematode Globodera rostochiensis. Three independent criteria were used for selection. First, genes of interest should predominantly be expressed in infective second-stage juveniles, and not, or to a far lesser extent, in younger developmental stages. For this, gene expression profiles from five different developmental stages were generated with cDNA-AFLP (amplified fragment length polymorphism). Secondly, the mRNA corresponding to such a putative pathogenicity factor should predominantly be present in the esophageal glands of pre-parasitic juveniles. This was checked by in situ hybridization. As a third criterion, these proteinaceous factors should be preceded by a signal peptide for secretion. Expression profiles of more than 4,000 genes were generated and three up-regulated, dorsal gland-specific proteins preceded by signal peptide for secretion were identified. No dorsal gland genes have been cloned before from plant-parasitic nematodes. The partial sequence of these three factors, A4, A18, and A41, showed no significant homology to any known gene. Their presence in the dorsal glands of infective juveniles suggests that these proteins could be involved in feeding cell initiation, and not in migration in the plant root or in protection against plant defense responses. Finally, the applicability of this new strategy in other plant-microbe interactions is discussed.https://apsjournals.apsnet.org/doi/10.1094/MPMI.2000.13.8.830 |
| spellingShingle | Ling Qin Hein Overmars Johannes Helder Herman Popeijus Jeroen Rouppe van der Voort Wouter Groenink Paul van Koert Arjen Schots Jaap Bakker Geert Smant An Efficient cDNA-AFLP-Based Strategy for the Identification of Putative Pathogenicity Factors from the Potato Cyst Nematode Globodera rostochiensis Molecular Plant-Microbe Interactions |
| title | An Efficient cDNA-AFLP-Based Strategy for the Identification of Putative Pathogenicity Factors from the Potato Cyst Nematode Globodera rostochiensis |
| title_full | An Efficient cDNA-AFLP-Based Strategy for the Identification of Putative Pathogenicity Factors from the Potato Cyst Nematode Globodera rostochiensis |
| title_fullStr | An Efficient cDNA-AFLP-Based Strategy for the Identification of Putative Pathogenicity Factors from the Potato Cyst Nematode Globodera rostochiensis |
| title_full_unstemmed | An Efficient cDNA-AFLP-Based Strategy for the Identification of Putative Pathogenicity Factors from the Potato Cyst Nematode Globodera rostochiensis |
| title_short | An Efficient cDNA-AFLP-Based Strategy for the Identification of Putative Pathogenicity Factors from the Potato Cyst Nematode Globodera rostochiensis |
| title_sort | efficient cdna aflp based strategy for the identification of putative pathogenicity factors from the potato cyst nematode globodera rostochiensis |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2000.13.8.830 |
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