Immune protection of three serine protease inhibitors vaccine in mice against Rhipicephalus sanguineus
Abstract Bioactive molecules in tick saliva are considered to be key to successful feeding and further the transmission of tick-borne pathogens. Problems such as pathogen transmission and animal weight loss result in tick infestation can cause tremendous economic losses to the livestock industry. Th...
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| Format: | Article |
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Nature Portfolio
2024-04-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-58303-4 |
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| author | Xiaoya Zhao Jianguo Zhao Jinhua Wang Chenghong Liao Qingfeng Guan Qian Han |
| author_facet | Xiaoya Zhao Jianguo Zhao Jinhua Wang Chenghong Liao Qingfeng Guan Qian Han |
| author_sort | Xiaoya Zhao |
| collection | DOAJ |
| description | Abstract Bioactive molecules in tick saliva are considered to be key to successful feeding and further the transmission of tick-borne pathogens. Problems such as pathogen transmission and animal weight loss result in tick infestation can cause tremendous economic losses to the livestock industry. Therefore, the development of a universal tick vaccine is urgently needed. In this paper, three serine protease inhibitor (serpin) proteins RMS-3, L7LRK7 and L7LTU1 were analyzed with bioinformatics methods. Subsequently the proteins were expressed and purified, and inoculated into Kunming mice for immune protection analysis. The amino acid sequence similarities between RMS-3, L7LRK7 and L7LTU1 were up to 90% in Rhipicephalus sanguineus. The recombinant RMS-3 + L7LRK7 + L7LTU1 showed anticoagulant reaction function and could inhibit the activity of CD4+ lymphocytes, when inoculated into Kunming mice. Additionally, After the immunized mice were challenged with Rhipicephalus sanguineus, the percentage of larvae and nymphs that were fully engorged dropped to 40.87% (P < 0.05) and 87.68% (P > 0.05) in the RmS-3 + L7LRK7 immune group, 49.57% (P < 0.01) and 52.06% (P < 0.05) in the RmS-3 + L7LTU1 group, and 45.22% (P < 0.05) and 60.28% (P < 0.05) in the RmS-3 + L7LRK7 + L7LTU1 immune group, in comparison with the control group. These data indicate that RmS-3 + L7LRK7 + L7LTU1 has good immune protection and has the potential to be developed into a vaccine against the larvae and nymphs of R. sanguineus. |
| first_indexed | 2024-04-24T12:39:51Z |
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| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-24T12:39:51Z |
| publishDate | 2024-04-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj.art-d40331079d244ee88e84d27020dd5dab2024-04-07T11:17:44ZengNature PortfolioScientific Reports2045-23222024-04-0114111110.1038/s41598-024-58303-4Immune protection of three serine protease inhibitors vaccine in mice against Rhipicephalus sanguineusXiaoya Zhao0Jianguo Zhao1Jinhua Wang2Chenghong Liao3Qingfeng Guan4Qian Han5Laboratory of Tropical Veterinary Medicine and Vector Biology, School of Life Sciences, Hainan UniversityLaboratory of Tropical Veterinary Medicine and Vector Biology, School of Life Sciences, Hainan UniversityLaboratory of Tropical Veterinary Medicine and Vector Biology, School of Life Sciences, Hainan UniversityLaboratory of Tropical Veterinary Medicine and Vector Biology, School of Life Sciences, Hainan UniversityLaboratory of Tropical Veterinary Medicine and Vector Biology, School of Life Sciences, Hainan UniversityLaboratory of Tropical Veterinary Medicine and Vector Biology, School of Life Sciences, Hainan UniversityAbstract Bioactive molecules in tick saliva are considered to be key to successful feeding and further the transmission of tick-borne pathogens. Problems such as pathogen transmission and animal weight loss result in tick infestation can cause tremendous economic losses to the livestock industry. Therefore, the development of a universal tick vaccine is urgently needed. In this paper, three serine protease inhibitor (serpin) proteins RMS-3, L7LRK7 and L7LTU1 were analyzed with bioinformatics methods. Subsequently the proteins were expressed and purified, and inoculated into Kunming mice for immune protection analysis. The amino acid sequence similarities between RMS-3, L7LRK7 and L7LTU1 were up to 90% in Rhipicephalus sanguineus. The recombinant RMS-3 + L7LRK7 + L7LTU1 showed anticoagulant reaction function and could inhibit the activity of CD4+ lymphocytes, when inoculated into Kunming mice. Additionally, After the immunized mice were challenged with Rhipicephalus sanguineus, the percentage of larvae and nymphs that were fully engorged dropped to 40.87% (P < 0.05) and 87.68% (P > 0.05) in the RmS-3 + L7LRK7 immune group, 49.57% (P < 0.01) and 52.06% (P < 0.05) in the RmS-3 + L7LTU1 group, and 45.22% (P < 0.05) and 60.28% (P < 0.05) in the RmS-3 + L7LRK7 + L7LTU1 immune group, in comparison with the control group. These data indicate that RmS-3 + L7LRK7 + L7LTU1 has good immune protection and has the potential to be developed into a vaccine against the larvae and nymphs of R. sanguineus.https://doi.org/10.1038/s41598-024-58303-4R. sanguineusSerpinsBioinformaticsImmune protectionVaccine |
| spellingShingle | Xiaoya Zhao Jianguo Zhao Jinhua Wang Chenghong Liao Qingfeng Guan Qian Han Immune protection of three serine protease inhibitors vaccine in mice against Rhipicephalus sanguineus Scientific Reports R. sanguineus Serpins Bioinformatics Immune protection Vaccine |
| title | Immune protection of three serine protease inhibitors vaccine in mice against Rhipicephalus sanguineus |
| title_full | Immune protection of three serine protease inhibitors vaccine in mice against Rhipicephalus sanguineus |
| title_fullStr | Immune protection of three serine protease inhibitors vaccine in mice against Rhipicephalus sanguineus |
| title_full_unstemmed | Immune protection of three serine protease inhibitors vaccine in mice against Rhipicephalus sanguineus |
| title_short | Immune protection of three serine protease inhibitors vaccine in mice against Rhipicephalus sanguineus |
| title_sort | immune protection of three serine protease inhibitors vaccine in mice against rhipicephalus sanguineus |
| topic | R. sanguineus Serpins Bioinformatics Immune protection Vaccine |
| url | https://doi.org/10.1038/s41598-024-58303-4 |
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