Use of aerobic treadmill exercises on nerve regeneration after sciatic nerve injury in spontaneously hypertensive rats
ABSTRACT Purpose: Various postoperative protocols have been proposed to improve outcomes and accelerate nerve regeneration. Recently, the use of physical exercise in a post-surgical neurorraphy procedure has shown good results when started early. We experimentally investigated the hypothesis that p...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2022-10-01
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| Series: | Acta Cirúrgica Brasileira |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502022000800202&tlng=en |
| _version_ | 1811192092563603456 |
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| author | Gustavo Santiago de Lima Figueiredo Marcela Fernandes Vinícius Neves Atti Sandra Gomes Valente Felipe Roth Luis Renato Nakachima João Baptista Gomes dos Santos Carlos Henrique Fernandes |
| author_facet | Gustavo Santiago de Lima Figueiredo Marcela Fernandes Vinícius Neves Atti Sandra Gomes Valente Felipe Roth Luis Renato Nakachima João Baptista Gomes dos Santos Carlos Henrique Fernandes |
| author_sort | Gustavo Santiago de Lima Figueiredo |
| collection | DOAJ |
| description | ABSTRACT Purpose: Various postoperative protocols have been proposed to improve outcomes and accelerate nerve regeneration. Recently, the use of physical exercise in a post-surgical neurorraphy procedure has shown good results when started early. We experimentally investigated the hypothesis that post-operative exercise speeds up results and improves clinical and morphologic parameters. Methods: Isogenic rats were randomly divided into four groups: 1 SHAM; 2 SHAM submitted to the exercise protocol (EP); 3 Grafting of the sciatic nerve; and 4 Grafting of the sciatic nerve associated with the EP. The EP was based on aerobic activities with a treadmill, with a progressive increase in time and intensity during 6 weeks. The results were evaluated by the sciatic functional index (SFI), morphometric and morphologic analysis of nerve distal to the lesion, and the number of spinal cord motor neurons, positive to the marker Fluoro-Gold (FG), captured retrogradely through neurorraphy. Results: Functional analysis (SFI) did not show a statistical difference between the group grafted with (–50.94) and without exercise (-65.79) after 90 days. The motoneurons count (Spinal cord histology) also showed no diference between these groups (834.5 × 833 respectively). Although functionally there is no difference between these groups, morphometric study showed a greater density (53.62) and larger fibers (7.762) in GRAFT group. When comparing both operated groups with both SHAM groups, all values were much lower. Conclusions: The experimental model that this aerobic treadmill exercises protocol did not modify nerve regeneration after sciatic nerve injury and repair with nerve graft. |
| first_indexed | 2024-04-11T23:45:58Z |
| format | Article |
| id | doaj.art-d405a41beb98428b856d6f6b2b07c207 |
| institution | Directory Open Access Journal |
| issn | 1678-2674 |
| language | English |
| last_indexed | 2024-04-11T23:45:58Z |
| publishDate | 2022-10-01 |
| publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| record_format | Article |
| series | Acta Cirúrgica Brasileira |
| spelling | doaj.art-d405a41beb98428b856d6f6b2b07c2072022-12-22T03:56:38ZengSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira1678-26742022-10-0137810.1590/acb370804Use of aerobic treadmill exercises on nerve regeneration after sciatic nerve injury in spontaneously hypertensive ratsGustavo Santiago de Lima Figueiredohttps://orcid.org/0000-0001-8708-7943Marcela Fernandeshttps://orcid.org/0000-0003-1646-6764Vinícius Neves Attihttps://orcid.org/0000-0003-2683-548XSandra Gomes Valentehttps://orcid.org/0000-0003-3004-6715Felipe Rothhttps://orcid.org/0000-0002-1146-8237Luis Renato Nakachimahttps://orcid.org/0000-0003-1901-9820João Baptista Gomes dos Santoshttps://orcid.org/0000-0003-0199-6578Carlos Henrique Fernandeshttps://orcid.org/0000-0003-0146-6091ABSTRACT Purpose: Various postoperative protocols have been proposed to improve outcomes and accelerate nerve regeneration. Recently, the use of physical exercise in a post-surgical neurorraphy procedure has shown good results when started early. We experimentally investigated the hypothesis that post-operative exercise speeds up results and improves clinical and morphologic parameters. Methods: Isogenic rats were randomly divided into four groups: 1 SHAM; 2 SHAM submitted to the exercise protocol (EP); 3 Grafting of the sciatic nerve; and 4 Grafting of the sciatic nerve associated with the EP. The EP was based on aerobic activities with a treadmill, with a progressive increase in time and intensity during 6 weeks. The results were evaluated by the sciatic functional index (SFI), morphometric and morphologic analysis of nerve distal to the lesion, and the number of spinal cord motor neurons, positive to the marker Fluoro-Gold (FG), captured retrogradely through neurorraphy. Results: Functional analysis (SFI) did not show a statistical difference between the group grafted with (–50.94) and without exercise (-65.79) after 90 days. The motoneurons count (Spinal cord histology) also showed no diference between these groups (834.5 × 833 respectively). Although functionally there is no difference between these groups, morphometric study showed a greater density (53.62) and larger fibers (7.762) in GRAFT group. When comparing both operated groups with both SHAM groups, all values were much lower. Conclusions: The experimental model that this aerobic treadmill exercises protocol did not modify nerve regeneration after sciatic nerve injury and repair with nerve graft.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502022000800202&tlng=enNerve fibersExercise TestInnervationMotor neurons |
| spellingShingle | Gustavo Santiago de Lima Figueiredo Marcela Fernandes Vinícius Neves Atti Sandra Gomes Valente Felipe Roth Luis Renato Nakachima João Baptista Gomes dos Santos Carlos Henrique Fernandes Use of aerobic treadmill exercises on nerve regeneration after sciatic nerve injury in spontaneously hypertensive rats Acta Cirúrgica Brasileira Nerve fibers Exercise Test Innervation Motor neurons |
| title | Use of aerobic treadmill exercises on nerve regeneration after sciatic nerve injury in spontaneously hypertensive rats |
| title_full | Use of aerobic treadmill exercises on nerve regeneration after sciatic nerve injury in spontaneously hypertensive rats |
| title_fullStr | Use of aerobic treadmill exercises on nerve regeneration after sciatic nerve injury in spontaneously hypertensive rats |
| title_full_unstemmed | Use of aerobic treadmill exercises on nerve regeneration after sciatic nerve injury in spontaneously hypertensive rats |
| title_short | Use of aerobic treadmill exercises on nerve regeneration after sciatic nerve injury in spontaneously hypertensive rats |
| title_sort | use of aerobic treadmill exercises on nerve regeneration after sciatic nerve injury in spontaneously hypertensive rats |
| topic | Nerve fibers Exercise Test Innervation Motor neurons |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502022000800202&tlng=en |
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