Agrobacterium-Mediated Transformation of Amaranthus cruentus L. Epicotils
Red amaranth Amaranthus cruentus L. is a valuable fodder and grain crop. To generate new varieties of this plant, genetic transformation methods can be used, but for A. cruentus such methods remain undeveloped. The present study describes the results of our research in Agrobacterium-mediated transfo...
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| Format: | Article |
| Language: | English |
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Siberian Federal University
2019-01-01
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| Series: | Журнал Сибирского федерального университета: Серия Биология |
| Subjects: | |
| Online Access: | http://elib.sfu-kras.ru/bitstream/2311/110078/1/04_Taipova.pdf |
| _version_ | 1819057003824152576 |
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| author | Ragida M. Taipova Khalit G. Musin Bulat R. Kuluev |
| author_facet | Ragida M. Taipova Khalit G. Musin Bulat R. Kuluev |
| author_sort | Ragida M. Taipova |
| collection | DOAJ |
| description | Red amaranth Amaranthus cruentus L. is a valuable fodder and grain crop. To generate new varieties of this plant, genetic transformation methods can be used, but for A. cruentus such methods remain undeveloped. The present study describes the results of our research in Agrobacterium-mediated transformation of epicotyl segments of A. cruentus variety “Bagryanyi” by the ARGOS-LIKE transgene of Arabidopsis thaliana controlled by the 35S promoter in the binary vector pCambia 1301 with a selective hygromycin B resistance gene. For shoot regeneration from epicotyl segments after Agrobacterium-mediated transformation, Murashige-Skoog (MS) medium containing 13 μM 6-benzylaminopurine and 1 μM 1-naphthylacetic acid was used. For the selection of transgenic shoots, 10 mg/L of hygromycin B was added to the MS medium. Rooting of shoots was performed on selective MS medium supplemented with 2 μM 3-indoleacetic acid. Three transgenic amaranth plants with the genetic engineering structure 35S::ARGOS-LIKE were generated. The efficiency of Agrobacterium-mediated transformation of A. cruentus was 4%. The amaranth plants transgenicity was confirmed by the PCR analysis for the presence of marker and target genes. Two transgenic plants were acclimatized to soil and open air conditions |
| first_indexed | 2024-12-21T13:32:24Z |
| format | Article |
| id | doaj.art-d40c263de3ee4de28f4ec31ae28a6269 |
| institution | Directory Open Access Journal |
| issn | 1997-1389 2313-5530 |
| language | English |
| last_indexed | 2024-12-21T13:32:24Z |
| publishDate | 2019-01-01 |
| publisher | Siberian Federal University |
| record_format | Article |
| series | Журнал Сибирского федерального университета: Серия Биология |
| spelling | doaj.art-d40c263de3ee4de28f4ec31ae28a62692022-12-21T19:02:16ZengSiberian Federal UniversityЖурнал Сибирского федерального университета: Серия Биология1997-13892313-55302019-01-01132179187http://elib.sfu-kras.ru/handle/2311/110078Agrobacterium-Mediated Transformation of Amaranthus cruentus L. EpicotilsRagida M. Taipova0Khalit G. Musin1Bulat R. Kuluev2Bashkir State University 32 Zaki Validi Str., Ufa, 450076, RussiaBashkir State University 32 Zaki Validi Str., Ufa, 450076, RussiaBashkir State University 32 Zaki Validi Str., Ufa, 450076, RussiaRed amaranth Amaranthus cruentus L. is a valuable fodder and grain crop. To generate new varieties of this plant, genetic transformation methods can be used, but for A. cruentus such methods remain undeveloped. The present study describes the results of our research in Agrobacterium-mediated transformation of epicotyl segments of A. cruentus variety “Bagryanyi” by the ARGOS-LIKE transgene of Arabidopsis thaliana controlled by the 35S promoter in the binary vector pCambia 1301 with a selective hygromycin B resistance gene. For shoot regeneration from epicotyl segments after Agrobacterium-mediated transformation, Murashige-Skoog (MS) medium containing 13 μM 6-benzylaminopurine and 1 μM 1-naphthylacetic acid was used. For the selection of transgenic shoots, 10 mg/L of hygromycin B was added to the MS medium. Rooting of shoots was performed on selective MS medium supplemented with 2 μM 3-indoleacetic acid. Three transgenic amaranth plants with the genetic engineering structure 35S::ARGOS-LIKE were generated. The efficiency of Agrobacterium-mediated transformation of A. cruentus was 4%. The amaranth plants transgenicity was confirmed by the PCR analysis for the presence of marker and target genes. Two transgenic plants were acclimatized to soil and open air conditionshttp://elib.sfu-kras.ru/bitstream/2311/110078/1/04_Taipova.pdfamaranthus cruentusin vitroargos-likeamaranthus cruentusred amaranthin vitroshoots regenerationagrobacterium-mediated transformationtransgenic plantsargos-like |
| spellingShingle | Ragida M. Taipova Khalit G. Musin Bulat R. Kuluev Agrobacterium-Mediated Transformation of Amaranthus cruentus L. Epicotils Журнал Сибирского федерального университета: Серия Биология amaranthus cruentus in vitro argos-like amaranthus cruentus red amaranth in vitro shoots regeneration agrobacterium-mediated transformation transgenic plants argos-like |
| title | Agrobacterium-Mediated Transformation of Amaranthus cruentus L. Epicotils |
| title_full | Agrobacterium-Mediated Transformation of Amaranthus cruentus L. Epicotils |
| title_fullStr | Agrobacterium-Mediated Transformation of Amaranthus cruentus L. Epicotils |
| title_full_unstemmed | Agrobacterium-Mediated Transformation of Amaranthus cruentus L. Epicotils |
| title_short | Agrobacterium-Mediated Transformation of Amaranthus cruentus L. Epicotils |
| title_sort | agrobacterium mediated transformation of amaranthus cruentus l epicotils |
| topic | amaranthus cruentus in vitro argos-like amaranthus cruentus red amaranth in vitro shoots regeneration agrobacterium-mediated transformation transgenic plants argos-like |
| url | http://elib.sfu-kras.ru/bitstream/2311/110078/1/04_Taipova.pdf |
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