Electroacupuncture Zusanli (ST36) Relieves Somatic Pain in Colitis Rats by Inhibiting Dorsal Root Ganglion Sympathetic-Sensory Coupling and Neurogenic Inflammation
Referred somatic pain triggered by hyperalgesia is common in patients with inflammatory bowel disease (IBD). It was reported that sprouting of sympathetic nerve fibers into the dorsal root ganglion (DGR) and neurogenic inflammation were related to neuropathic pain, the excitability of neurons, and a...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hindawi Limited
2023-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2023/9303419 |
| _version_ | 1797871890228838400 |
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| author | Yi-li Wang Hai-yan Zhu Xi-qian Lv Xing-ying Ren Ying-chun Peng Jin-yu Qu Xue-fang Shen Ran Sun Meng-lu Xiao Hong Zhang Zhao-hui Chen Peng Cong |
| author_facet | Yi-li Wang Hai-yan Zhu Xi-qian Lv Xing-ying Ren Ying-chun Peng Jin-yu Qu Xue-fang Shen Ran Sun Meng-lu Xiao Hong Zhang Zhao-hui Chen Peng Cong |
| author_sort | Yi-li Wang |
| collection | DOAJ |
| description | Referred somatic pain triggered by hyperalgesia is common in patients with inflammatory bowel disease (IBD). It was reported that sprouting of sympathetic nerve fibers into the dorsal root ganglion (DGR) and neurogenic inflammation were related to neuropathic pain, the excitability of neurons, and afferents. The purpose of the study was to explore the potential and mechanism of electroacupuncture (EA) at Zusanli (ST36) for the intervention of colon inflammation and hyperalgesia. Sprague-Dawley (SD) was randomly divided into four groups, including control, model, EA, and sham-EA. Our results showed EA treatment significantly attenuated dextran sulfate sodium- (DSS-) induced colorectal lesions and inflammatory cytokine secretion, such as TNF-α, IL-1β, PGE2, and IL-6. EA also inhibited mechanical and thermal pain hypersensitivities of colitis rats. Importantly, EA effectively abrogated the promotion effect of DSS on ipsilateral lumbar 6 (L6) DRG sympathetic-sensory coupling, manifested as the sprouting of tyrosine hydroxylase- (TH-) positive sympathetic fibers into sensory neurons and colocalization of and calcitonin gene-related peptide (CGRP). Furthermore, EA at Zusanli (ST36) activated neurogenic inflammation, characterized by decreased expression of substance P (SP), hyaluronic acid (HA), bradykinin (BK), and prostacyclin (PGI2) in colitis rat skin tissues corresponding to the L6 DRG. Mechanically, EA treatment reduced the activation of the TRPV1/CGRP, ERK, and TLR4 signaling pathways in L6 DRG of colitis rats. Taken together, we presumed that EA treatment improved colon inflammation and hyperalgesia, potentially by suppressing the sprouting of sympathetic nerve fibers into the L6 DGR and neurogenic inflammation via deactivating the TRPV1/CGRP, ERK, and TLR4 signaling pathways. |
| first_indexed | 2024-04-10T00:50:09Z |
| format | Article |
| id | doaj.art-d40ce96362dc4449a1d4ea6606e36f23 |
| institution | Directory Open Access Journal |
| issn | 1687-5443 |
| language | English |
| last_indexed | 2024-04-10T00:50:09Z |
| publishDate | 2023-01-01 |
| publisher | Hindawi Limited |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj.art-d40ce96362dc4449a1d4ea6606e36f232023-03-13T11:25:52ZengHindawi LimitedNeural Plasticity1687-54432023-01-01202310.1155/2023/9303419Electroacupuncture Zusanli (ST36) Relieves Somatic Pain in Colitis Rats by Inhibiting Dorsal Root Ganglion Sympathetic-Sensory Coupling and Neurogenic InflammationYi-li Wang0Hai-yan Zhu1Xi-qian Lv2Xing-ying Ren3Ying-chun Peng4Jin-yu Qu5Xue-fang Shen6Ran Sun7Meng-lu Xiao8Hong Zhang9Zhao-hui Chen10Peng Cong11Chengdu Medical CollegeChengdu Medical CollegeChengdu Medical CollegeChengdu Medical CollegeChengdu Medical CollegeChengdu Medical CollegeChengdu Medical CollegeChengdu University of Traditional Chinese MedicineChengdu University of Traditional Chinese MedicineChengdu University of Traditional Chinese MedicineChengdu Medical CollegeChengdu Medical CollegeReferred somatic pain triggered by hyperalgesia is common in patients with inflammatory bowel disease (IBD). It was reported that sprouting of sympathetic nerve fibers into the dorsal root ganglion (DGR) and neurogenic inflammation were related to neuropathic pain, the excitability of neurons, and afferents. The purpose of the study was to explore the potential and mechanism of electroacupuncture (EA) at Zusanli (ST36) for the intervention of colon inflammation and hyperalgesia. Sprague-Dawley (SD) was randomly divided into four groups, including control, model, EA, and sham-EA. Our results showed EA treatment significantly attenuated dextran sulfate sodium- (DSS-) induced colorectal lesions and inflammatory cytokine secretion, such as TNF-α, IL-1β, PGE2, and IL-6. EA also inhibited mechanical and thermal pain hypersensitivities of colitis rats. Importantly, EA effectively abrogated the promotion effect of DSS on ipsilateral lumbar 6 (L6) DRG sympathetic-sensory coupling, manifested as the sprouting of tyrosine hydroxylase- (TH-) positive sympathetic fibers into sensory neurons and colocalization of and calcitonin gene-related peptide (CGRP). Furthermore, EA at Zusanli (ST36) activated neurogenic inflammation, characterized by decreased expression of substance P (SP), hyaluronic acid (HA), bradykinin (BK), and prostacyclin (PGI2) in colitis rat skin tissues corresponding to the L6 DRG. Mechanically, EA treatment reduced the activation of the TRPV1/CGRP, ERK, and TLR4 signaling pathways in L6 DRG of colitis rats. Taken together, we presumed that EA treatment improved colon inflammation and hyperalgesia, potentially by suppressing the sprouting of sympathetic nerve fibers into the L6 DGR and neurogenic inflammation via deactivating the TRPV1/CGRP, ERK, and TLR4 signaling pathways.http://dx.doi.org/10.1155/2023/9303419 |
| spellingShingle | Yi-li Wang Hai-yan Zhu Xi-qian Lv Xing-ying Ren Ying-chun Peng Jin-yu Qu Xue-fang Shen Ran Sun Meng-lu Xiao Hong Zhang Zhao-hui Chen Peng Cong Electroacupuncture Zusanli (ST36) Relieves Somatic Pain in Colitis Rats by Inhibiting Dorsal Root Ganglion Sympathetic-Sensory Coupling and Neurogenic Inflammation Neural Plasticity |
| title | Electroacupuncture Zusanli (ST36) Relieves Somatic Pain in Colitis Rats by Inhibiting Dorsal Root Ganglion Sympathetic-Sensory Coupling and Neurogenic Inflammation |
| title_full | Electroacupuncture Zusanli (ST36) Relieves Somatic Pain in Colitis Rats by Inhibiting Dorsal Root Ganglion Sympathetic-Sensory Coupling and Neurogenic Inflammation |
| title_fullStr | Electroacupuncture Zusanli (ST36) Relieves Somatic Pain in Colitis Rats by Inhibiting Dorsal Root Ganglion Sympathetic-Sensory Coupling and Neurogenic Inflammation |
| title_full_unstemmed | Electroacupuncture Zusanli (ST36) Relieves Somatic Pain in Colitis Rats by Inhibiting Dorsal Root Ganglion Sympathetic-Sensory Coupling and Neurogenic Inflammation |
| title_short | Electroacupuncture Zusanli (ST36) Relieves Somatic Pain in Colitis Rats by Inhibiting Dorsal Root Ganglion Sympathetic-Sensory Coupling and Neurogenic Inflammation |
| title_sort | electroacupuncture zusanli st36 relieves somatic pain in colitis rats by inhibiting dorsal root ganglion sympathetic sensory coupling and neurogenic inflammation |
| url | http://dx.doi.org/10.1155/2023/9303419 |
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