Reliable particle sizing in vaccine formulations using advanced dynamic light scattering
Understanding the impact of lipid nanoparticle size on immunogenicity represents an important step for enabling the rapid development of novel vaccines against known or emergent diseases. Dynamic light scattering, also known as quasi-elastic light scattering or photon correlation spectroscopy, has e...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Analytical Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/frans.2024.1358893/full |
| _version_ | 1797291664832724992 |
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| author | Coline Bretz Andrea Jauslin Dario Leumann Marius Koch Andrea Vaccaro |
| author_facet | Coline Bretz Andrea Jauslin Dario Leumann Marius Koch Andrea Vaccaro |
| author_sort | Coline Bretz |
| collection | DOAJ |
| description | Understanding the impact of lipid nanoparticle size on immunogenicity represents an important step for enabling the rapid development of novel vaccines against known or emergent diseases. Dynamic light scattering, also known as quasi-elastic light scattering or photon correlation spectroscopy, has established itself as an optimal analytical method to determine particle size due to its in-situ approach and fast measurements. However, its application to many systems of industrial relevance has been hindered due to artifacts arising from multiple scattering. Result interpretation becomes severely compromised depending on the concentration of the system and the size of the particles. In this context, strong sample dilution is often required, bringing additional uncertainties to the formulation development process. Here, we show how advanced dynamic light scattering technology can filter out multiple scattering from the signal and yield fully accurate sizing measurements regardless of the sample concentration. We illustrate this in a comparative study with standard dynamic light scattering using polystyrene beads as model suspension as well as a concentrated commercial lipid nanoparticle adjuvant (AddaVax™). |
| first_indexed | 2024-03-07T19:40:08Z |
| format | Article |
| id | doaj.art-d40d532759ac4c7d8ae97643b7cf28b7 |
| institution | Directory Open Access Journal |
| issn | 2673-9283 |
| language | English |
| last_indexed | 2024-03-07T19:40:08Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Analytical Science |
| spelling | doaj.art-d40d532759ac4c7d8ae97643b7cf28b72024-02-29T05:36:19ZengFrontiers Media S.A.Frontiers in Analytical Science2673-92832024-02-01410.3389/frans.2024.13588931358893Reliable particle sizing in vaccine formulations using advanced dynamic light scatteringColine Bretz0Andrea Jauslin1Dario Leumann2Marius Koch3Andrea Vaccaro4LS Instruments AG, Fribourg, SwitzerlandSolvias AG, Large Molecule & ATMP Services, Kaiseraugst, SwitzerlandLS Instruments AG, Fribourg, SwitzerlandSolvias AG, Large Molecule & ATMP Services, Kaiseraugst, SwitzerlandLS Instruments AG, Fribourg, SwitzerlandUnderstanding the impact of lipid nanoparticle size on immunogenicity represents an important step for enabling the rapid development of novel vaccines against known or emergent diseases. Dynamic light scattering, also known as quasi-elastic light scattering or photon correlation spectroscopy, has established itself as an optimal analytical method to determine particle size due to its in-situ approach and fast measurements. However, its application to many systems of industrial relevance has been hindered due to artifacts arising from multiple scattering. Result interpretation becomes severely compromised depending on the concentration of the system and the size of the particles. In this context, strong sample dilution is often required, bringing additional uncertainties to the formulation development process. Here, we show how advanced dynamic light scattering technology can filter out multiple scattering from the signal and yield fully accurate sizing measurements regardless of the sample concentration. We illustrate this in a comparative study with standard dynamic light scattering using polystyrene beads as model suspension as well as a concentrated commercial lipid nanoparticle adjuvant (AddaVax™).https://www.frontiersin.org/articles/10.3389/frans.2024.1358893/fullbioanalytical methodsbiopolymers/lipidsnanoparticles/nanotechnologymultiple scatteringdynamic light scatteringcross-correlation |
| spellingShingle | Coline Bretz Andrea Jauslin Dario Leumann Marius Koch Andrea Vaccaro Reliable particle sizing in vaccine formulations using advanced dynamic light scattering Frontiers in Analytical Science bioanalytical methods biopolymers/lipids nanoparticles/nanotechnology multiple scattering dynamic light scattering cross-correlation |
| title | Reliable particle sizing in vaccine formulations using advanced dynamic light scattering |
| title_full | Reliable particle sizing in vaccine formulations using advanced dynamic light scattering |
| title_fullStr | Reliable particle sizing in vaccine formulations using advanced dynamic light scattering |
| title_full_unstemmed | Reliable particle sizing in vaccine formulations using advanced dynamic light scattering |
| title_short | Reliable particle sizing in vaccine formulations using advanced dynamic light scattering |
| title_sort | reliable particle sizing in vaccine formulations using advanced dynamic light scattering |
| topic | bioanalytical methods biopolymers/lipids nanoparticles/nanotechnology multiple scattering dynamic light scattering cross-correlation |
| url | https://www.frontiersin.org/articles/10.3389/frans.2024.1358893/full |
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