The Membrane Proteome of Spores and Vegetative Cells of the Food-Borne Pathogen <i>Bacillus cereus</i>
Membrane proteins are fascinating since they play an important role in diverse cellular functions and constitute many drug targets. Membrane proteins are challenging to analyze. The spore, the most resistant form of known life, harbors a compressed inner membrane. This membrane acts not only as a ba...
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| Format: | Article |
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MDPI AG
2021-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/22/12475 |
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| author | Xiaowei Gao Bhagyashree N. Swarge Henk L. Dekker Winfried Roseboom Stanley Brul Gertjan Kramer |
| author_facet | Xiaowei Gao Bhagyashree N. Swarge Henk L. Dekker Winfried Roseboom Stanley Brul Gertjan Kramer |
| author_sort | Xiaowei Gao |
| collection | DOAJ |
| description | Membrane proteins are fascinating since they play an important role in diverse cellular functions and constitute many drug targets. Membrane proteins are challenging to analyze. The spore, the most resistant form of known life, harbors a compressed inner membrane. This membrane acts not only as a barrier for undesired molecules but also as a scaffold for proteins involved in signal transduction and the transport of metabolites during spore germination and subsequent vegetative growth. In this study, we adapted a membrane enrichment method to study the membrane proteome of spores and cells of the food-borne pathogen <i>Bacillus cereus</i> using quantitative proteomics. Using bioinformatics filtering we identify and quantify 498 vegetative cell membrane proteins and 244 spore inner membrane proteins. Comparison of vegetative and spore membrane proteins showed there were 54 spore membrane-specific and 308 cell membrane-specific proteins. Functional characterization of these proteins showed that the cell membrane proteome has a far larger number of transporters, receptors and proteins related to cell division and motility. This was also reflected in the much higher expression level of many of these proteins in the cellular membrane for those proteins that were in common with the spore inner membrane. The spore inner membrane had specific expression of several germinant receptors and spore-specific proteins, but also seemed to show a preference towards the use of simple carbohydrates like glucose and fructose owing to only expressing transporters for these. These results show the differences in membrane proteome composition and show us the specific proteins necessary in the inner membrane of a dormant spore of this toxigenic spore-forming bacterium to survive adverse conditions. |
| first_indexed | 2024-03-10T05:26:03Z |
| format | Article |
| id | doaj.art-d40e708adac44cbd888ea30063ce4273 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T05:26:03Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-d40e708adac44cbd888ea30063ce42732023-11-22T23:42:57ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-11-0122221247510.3390/ijms222212475The Membrane Proteome of Spores and Vegetative Cells of the Food-Borne Pathogen <i>Bacillus cereus</i>Xiaowei Gao0Bhagyashree N. Swarge1Henk L. Dekker2Winfried Roseboom3Stanley Brul4Gertjan Kramer5Molecular Biology & Microbial Food Safety, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The NetherlandsMolecular Biology & Microbial Food Safety, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The NetherlandsMass Spectrometry of Biomolecules, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The NetherlandsMass Spectrometry of Biomolecules, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The NetherlandsMolecular Biology & Microbial Food Safety, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The NetherlandsMass Spectrometry of Biomolecules, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The NetherlandsMembrane proteins are fascinating since they play an important role in diverse cellular functions and constitute many drug targets. Membrane proteins are challenging to analyze. The spore, the most resistant form of known life, harbors a compressed inner membrane. This membrane acts not only as a barrier for undesired molecules but also as a scaffold for proteins involved in signal transduction and the transport of metabolites during spore germination and subsequent vegetative growth. In this study, we adapted a membrane enrichment method to study the membrane proteome of spores and cells of the food-borne pathogen <i>Bacillus cereus</i> using quantitative proteomics. Using bioinformatics filtering we identify and quantify 498 vegetative cell membrane proteins and 244 spore inner membrane proteins. Comparison of vegetative and spore membrane proteins showed there were 54 spore membrane-specific and 308 cell membrane-specific proteins. Functional characterization of these proteins showed that the cell membrane proteome has a far larger number of transporters, receptors and proteins related to cell division and motility. This was also reflected in the much higher expression level of many of these proteins in the cellular membrane for those proteins that were in common with the spore inner membrane. The spore inner membrane had specific expression of several germinant receptors and spore-specific proteins, but also seemed to show a preference towards the use of simple carbohydrates like glucose and fructose owing to only expressing transporters for these. These results show the differences in membrane proteome composition and show us the specific proteins necessary in the inner membrane of a dormant spore of this toxigenic spore-forming bacterium to survive adverse conditions.https://www.mdpi.com/1422-0067/22/22/12475<i>Bacillus cereus</i>spore inner membraneproteome |
| spellingShingle | Xiaowei Gao Bhagyashree N. Swarge Henk L. Dekker Winfried Roseboom Stanley Brul Gertjan Kramer The Membrane Proteome of Spores and Vegetative Cells of the Food-Borne Pathogen <i>Bacillus cereus</i> International Journal of Molecular Sciences <i>Bacillus cereus</i> spore inner membrane proteome |
| title | The Membrane Proteome of Spores and Vegetative Cells of the Food-Borne Pathogen <i>Bacillus cereus</i> |
| title_full | The Membrane Proteome of Spores and Vegetative Cells of the Food-Borne Pathogen <i>Bacillus cereus</i> |
| title_fullStr | The Membrane Proteome of Spores and Vegetative Cells of the Food-Borne Pathogen <i>Bacillus cereus</i> |
| title_full_unstemmed | The Membrane Proteome of Spores and Vegetative Cells of the Food-Borne Pathogen <i>Bacillus cereus</i> |
| title_short | The Membrane Proteome of Spores and Vegetative Cells of the Food-Borne Pathogen <i>Bacillus cereus</i> |
| title_sort | membrane proteome of spores and vegetative cells of the food borne pathogen i bacillus cereus i |
| topic | <i>Bacillus cereus</i> spore inner membrane proteome |
| url | https://www.mdpi.com/1422-0067/22/22/12475 |
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