Fecal microbiota transplantation repairs intestinal permeability and regulates the expression of 5-HT to influence alcohol-induced depression-like behaviors in C57BL/6J mice
The epidemic of alcohol abuse affects millions of people worldwide. Relevant evidence supports the notion that the gut microbiota (GM) plays a crucial role in central nervous system (CNS) function, and its composition undergoes changes following alcohol consumption. Therefore, the purpose of this st...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1241309/full |
| _version_ | 1797365540576034816 |
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| author | Dezhi Li Wei Liang Wentong Zhang Zhiqiang Huang Haipeng Liang Qing Liu |
| author_facet | Dezhi Li Wei Liang Wentong Zhang Zhiqiang Huang Haipeng Liang Qing Liu |
| author_sort | Dezhi Li |
| collection | DOAJ |
| description | The epidemic of alcohol abuse affects millions of people worldwide. Relevant evidence supports the notion that the gut microbiota (GM) plays a crucial role in central nervous system (CNS) function, and its composition undergoes changes following alcohol consumption. Therefore, the purpose of this study was to investigate the effect of reconstructing the gut microbiota by fecal microbiota transplantation (FMT) on alcohol dependence. Here, we established an alcohol dependence model with C57BL/6J mice and proved that FMT treatment improved anxiety-like behavior and alcohol-seeking behavior in alcohol-dependent mice. Additionally, we found that the expression of the intestinal intercellular tight junction structure proteins ZO-1 and occludin was significantly increased after FMT. FMT repaired intestinal permeability in alcohol-dependent mice and decreased the levels of lipopolysaccharide (LPS) and proinflammatory factors. Moreover, the serotonin (5-hydroxytryptamine, 5-HT) content was significantly increased in alcohol-dependent mouse intestinal and brain tissues after receiving the fecal microbiome from healthy mice. 16S rRNA sequencing demonstrated that FMT markedly reshaped the composition of the gut microbiota and elicited changes in the intestinal barrier and 5-HT levels. Collectively, our results revealed that FMT has a palliative effect on alcohol dependence and explored the underlying mechanisms, which provides new strategies for the treatment of alcohol dependence. |
| first_indexed | 2024-03-08T16:50:27Z |
| format | Article |
| id | doaj.art-d40fc6567b844eaabdd31e155426b502 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-08T16:50:27Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-d40fc6567b844eaabdd31e155426b5022024-01-05T04:59:11ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-01-011410.3389/fmicb.2023.12413091241309Fecal microbiota transplantation repairs intestinal permeability and regulates the expression of 5-HT to influence alcohol-induced depression-like behaviors in C57BL/6J miceDezhi Li0Wei Liang1Wentong Zhang2Zhiqiang Huang3Haipeng Liang4Qing Liu5School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, ChinaThe Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou, ChinaSchool of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, ChinaSchool of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, ChinaQingyang City People's Hospital General Surgery, Qingyang, ChinaSchool of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, ChinaThe epidemic of alcohol abuse affects millions of people worldwide. Relevant evidence supports the notion that the gut microbiota (GM) plays a crucial role in central nervous system (CNS) function, and its composition undergoes changes following alcohol consumption. Therefore, the purpose of this study was to investigate the effect of reconstructing the gut microbiota by fecal microbiota transplantation (FMT) on alcohol dependence. Here, we established an alcohol dependence model with C57BL/6J mice and proved that FMT treatment improved anxiety-like behavior and alcohol-seeking behavior in alcohol-dependent mice. Additionally, we found that the expression of the intestinal intercellular tight junction structure proteins ZO-1 and occludin was significantly increased after FMT. FMT repaired intestinal permeability in alcohol-dependent mice and decreased the levels of lipopolysaccharide (LPS) and proinflammatory factors. Moreover, the serotonin (5-hydroxytryptamine, 5-HT) content was significantly increased in alcohol-dependent mouse intestinal and brain tissues after receiving the fecal microbiome from healthy mice. 16S rRNA sequencing demonstrated that FMT markedly reshaped the composition of the gut microbiota and elicited changes in the intestinal barrier and 5-HT levels. Collectively, our results revealed that FMT has a palliative effect on alcohol dependence and explored the underlying mechanisms, which provides new strategies for the treatment of alcohol dependence.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1241309/fullalcohol dependencefecal microbiota transplantation5-HTbrain gut dysfunctiongut microbiota |
| spellingShingle | Dezhi Li Wei Liang Wentong Zhang Zhiqiang Huang Haipeng Liang Qing Liu Fecal microbiota transplantation repairs intestinal permeability and regulates the expression of 5-HT to influence alcohol-induced depression-like behaviors in C57BL/6J mice Frontiers in Microbiology alcohol dependence fecal microbiota transplantation 5-HT brain gut dysfunction gut microbiota |
| title | Fecal microbiota transplantation repairs intestinal permeability and regulates the expression of 5-HT to influence alcohol-induced depression-like behaviors in C57BL/6J mice |
| title_full | Fecal microbiota transplantation repairs intestinal permeability and regulates the expression of 5-HT to influence alcohol-induced depression-like behaviors in C57BL/6J mice |
| title_fullStr | Fecal microbiota transplantation repairs intestinal permeability and regulates the expression of 5-HT to influence alcohol-induced depression-like behaviors in C57BL/6J mice |
| title_full_unstemmed | Fecal microbiota transplantation repairs intestinal permeability and regulates the expression of 5-HT to influence alcohol-induced depression-like behaviors in C57BL/6J mice |
| title_short | Fecal microbiota transplantation repairs intestinal permeability and regulates the expression of 5-HT to influence alcohol-induced depression-like behaviors in C57BL/6J mice |
| title_sort | fecal microbiota transplantation repairs intestinal permeability and regulates the expression of 5 ht to influence alcohol induced depression like behaviors in c57bl 6j mice |
| topic | alcohol dependence fecal microbiota transplantation 5-HT brain gut dysfunction gut microbiota |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1241309/full |
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