Tumour-Associated Tissue Eosinophilia in Oral Squamous Cell Carcinoma- A Boon or a Bane?
Introduction: The infiltration of tumour stroma by eosinophils, Tumour-Associated Tissue Eosinophilia (TATE) is known to modulate the evolution of Oral Squamous Cell Carcinoma (OSCC). Identification of eosinophils in the inflammatory stroma has been proven to be an important factor in prognostic...
Main Authors: | , , , , , , , |
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Format: | Article |
Language: | English |
Published: |
JCDR Research and Publications Private Limited
2016-04-01
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Series: | Journal of Clinical and Diagnostic Research |
Subjects: | |
Online Access: | https://jcdr.net/articles/PDF/7637/16440_CE[Ra1]_F(P)_PF1(EKAK)_PFA(AK)_PF2(PAG).pdf |
Summary: | Introduction: The infiltration of tumour stroma by eosinophils,
Tumour-Associated Tissue Eosinophilia (TATE) is known to
modulate the evolution of Oral Squamous Cell Carcinoma
(OSCC). Identification of eosinophils in the inflammatory stroma
has been proven to be an important factor in prognostication
of malignant tumours including cancers of mouth, oesophagus,
larynx, pharynx, breast, lung, intestine and genitourinary tract.
Aim: Our study aimed to assess the role of TATE as a
prognosticator in OSCC as visualized by Haematoxylin and
Eosin (H&E) and congo red staining.
Materials and Methods: Thirty histologically-proven cases of
OSCC were retrieved from the archives of Department of Oral
Pathology, Manipal College of Dental Sciences, Mangalore,
Manipal University, Karnataka, India. Two serial sections of 4µm
thickness were made and subjected to routine staining with H&E
and modified congo red staining, where eosinophil granules
stained red and nuclei stained blue. In 40x magnification, 10 HPF
at invasive tumour front were assessed for counting eosinophils
by placing a 49 square grid (measuring 0.0289 sq mm).
Statistical Analysis: The TATE was compared with the
prognosticators using Mann-Whitney U-test. The grades of
carcinoma were correlated with TATE using Kruskal-Wallis test
followed by Post-hoc Bonferronis correction. Agreement of the
number of eosinophils counted in the two staining techniques
(H&E and Congo red) in OSCC was achieved using interclass
correlation coefficient, and Friedman’s test. A value of p< 0.05
was considered statistically significant.
Results: Our results showed that tissue eosinophil counts
were higher in well-differentiated cases of OSCC, cases with
lymph node involvement, decreased survival, without margin
involvement and in cases that did not recur. H&E stain showed
significantly better visualization of eosinophils resulting in higher
eosinophil counts than when seen with Congo red (p=0.008).
Conclusion: Thus, TATE can be used as a surrogate marker in
prediction of survival and recurrence in OSCC. H&E proved to
be a better stain for evaluation of eosinophils. |
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ISSN: | 2249-782X 0973-709X |