Rapid visual characterization of alkaloid changes in traditional processing of Tibetan medicine Aconitum pendulum by high-performance thin-layer chromatography coupled with desorption electrospray ionization mass spectrometry imaging
Radix Aconiti, also known as Tie-bang-chui (TBC), Pang-a-na-bao, and Bang-na, is a typical aconitum Tibetan medicine and a perennial herb of the genus Aconitum pendulum Busch. and A. flavum Hand. -Mazz. dry roots. It has high toxicity and remarkable efficacy; as such, it is a typical “highly toxic a...
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2023.1104473/full |
| _version_ | 1797843339404378112 |
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| author | Xiaoyan Tan Qingxiu He Zhaoqing Pei Yue Liu Zige Feng Congying Li Ce Tang Ce Tang Yi Zhang Yi Zhang |
| author_facet | Xiaoyan Tan Qingxiu He Zhaoqing Pei Yue Liu Zige Feng Congying Li Ce Tang Ce Tang Yi Zhang Yi Zhang |
| author_sort | Xiaoyan Tan |
| collection | DOAJ |
| description | Radix Aconiti, also known as Tie-bang-chui (TBC), Pang-a-na-bao, and Bang-na, is a typical aconitum Tibetan medicine and a perennial herb of the genus Aconitum pendulum Busch. and A. flavum Hand. -Mazz. dry roots. It has high toxicity and remarkable efficacy; as such, it is a typical “highly toxic and effective” drug that needs be processed and used. Processing methods of this Tibetan medicine include non-heating of highland barley wine (HBW) and fructus chebulae soup (FCS). This work aimed to understand differences in chemical composition between non-heat processed products and raw TBC. In this study, high-performance thin-layer chromatography (HPTLC) and desorption electrospray ionization mass spectrometry imaging (DESI-MSI) were used to analyze the chemical composition of TBC processed by FCS (F-TBC) and HBW (H-TBC). The MRM mode of HPLC-QqQ-MS/MS was selected to determine the changes of several representative alkaloids to comparison with the former results. A total of 52 chemical constituents were identified in raw and processed products, and the chemical composition of F-TBC and H-TBC changed slightly compared with that of raw TBC. The processing mechanism of H-TBC was also different from that of F-TBC, which might be related to the large amount of acidic tannins in FCS. It was found that the content of all six alkaloids decreased after processing by FCS, and all five alkaloids decreased except aconitine increased after processing by HBW. The combination of HPTLC and DESI-MSI could be an effective method for rapid identification of chemical components and changing rules in ethnic medicine. The wide application of this technology provides not only an alternative method for the traditional separation and identification of secondary metabolism but also a reference for research on the processing mechanism and quality control of ethnic medicine. |
| first_indexed | 2024-04-09T17:03:34Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-09T17:03:34Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Pharmacology |
| spelling | doaj.art-d4131d0abc594f09b60db8ef96c3924a2023-04-21T04:18:14ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122023-04-011410.3389/fphar.2023.11044731104473Rapid visual characterization of alkaloid changes in traditional processing of Tibetan medicine Aconitum pendulum by high-performance thin-layer chromatography coupled with desorption electrospray ionization mass spectrometry imagingXiaoyan Tan0Qingxiu He1Zhaoqing Pei2Yue Liu3Zige Feng4Congying Li5Ce Tang6Ce Tang7Yi Zhang8Yi Zhang9School of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaSchool of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaInnovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaSchool of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaSchool of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaSchool of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaSchool of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaMeishan Hosptial of Chengdu University of Traditional Chinese Medicine, Meishan, ChinaSchool of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaMeishan Hosptial of Chengdu University of Traditional Chinese Medicine, Meishan, ChinaRadix Aconiti, also known as Tie-bang-chui (TBC), Pang-a-na-bao, and Bang-na, is a typical aconitum Tibetan medicine and a perennial herb of the genus Aconitum pendulum Busch. and A. flavum Hand. -Mazz. dry roots. It has high toxicity and remarkable efficacy; as such, it is a typical “highly toxic and effective” drug that needs be processed and used. Processing methods of this Tibetan medicine include non-heating of highland barley wine (HBW) and fructus chebulae soup (FCS). This work aimed to understand differences in chemical composition between non-heat processed products and raw TBC. In this study, high-performance thin-layer chromatography (HPTLC) and desorption electrospray ionization mass spectrometry imaging (DESI-MSI) were used to analyze the chemical composition of TBC processed by FCS (F-TBC) and HBW (H-TBC). The MRM mode of HPLC-QqQ-MS/MS was selected to determine the changes of several representative alkaloids to comparison with the former results. A total of 52 chemical constituents were identified in raw and processed products, and the chemical composition of F-TBC and H-TBC changed slightly compared with that of raw TBC. The processing mechanism of H-TBC was also different from that of F-TBC, which might be related to the large amount of acidic tannins in FCS. It was found that the content of all six alkaloids decreased after processing by FCS, and all five alkaloids decreased except aconitine increased after processing by HBW. The combination of HPTLC and DESI-MSI could be an effective method for rapid identification of chemical components and changing rules in ethnic medicine. The wide application of this technology provides not only an alternative method for the traditional separation and identification of secondary metabolism but also a reference for research on the processing mechanism and quality control of ethnic medicine.https://www.frontiersin.org/articles/10.3389/fphar.2023.1104473/fullDESI-MSIHPTLCradix aconitiprocessingalkaloids |
| spellingShingle | Xiaoyan Tan Qingxiu He Zhaoqing Pei Yue Liu Zige Feng Congying Li Ce Tang Ce Tang Yi Zhang Yi Zhang Rapid visual characterization of alkaloid changes in traditional processing of Tibetan medicine Aconitum pendulum by high-performance thin-layer chromatography coupled with desorption electrospray ionization mass spectrometry imaging Frontiers in Pharmacology DESI-MSI HPTLC radix aconiti processing alkaloids |
| title | Rapid visual characterization of alkaloid changes in traditional processing of Tibetan medicine Aconitum pendulum by high-performance thin-layer chromatography coupled with desorption electrospray ionization mass spectrometry imaging |
| title_full | Rapid visual characterization of alkaloid changes in traditional processing of Tibetan medicine Aconitum pendulum by high-performance thin-layer chromatography coupled with desorption electrospray ionization mass spectrometry imaging |
| title_fullStr | Rapid visual characterization of alkaloid changes in traditional processing of Tibetan medicine Aconitum pendulum by high-performance thin-layer chromatography coupled with desorption electrospray ionization mass spectrometry imaging |
| title_full_unstemmed | Rapid visual characterization of alkaloid changes in traditional processing of Tibetan medicine Aconitum pendulum by high-performance thin-layer chromatography coupled with desorption electrospray ionization mass spectrometry imaging |
| title_short | Rapid visual characterization of alkaloid changes in traditional processing of Tibetan medicine Aconitum pendulum by high-performance thin-layer chromatography coupled with desorption electrospray ionization mass spectrometry imaging |
| title_sort | rapid visual characterization of alkaloid changes in traditional processing of tibetan medicine aconitum pendulum by high performance thin layer chromatography coupled with desorption electrospray ionization mass spectrometry imaging |
| topic | DESI-MSI HPTLC radix aconiti processing alkaloids |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2023.1104473/full |
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