Biotransformation of caffeic acid into a promising biologically active metabolite by Candida albicans isolate CI-24
In this study, a total number of 92 clinical isolates of Candida species was used to test their ability to transform caffeic acid using the two-stage fermentation protocol. The success of the biotransformation process was confirmed by TLC autography method and nuclear magnetic resonance analysis. Th...
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| Format: | Article |
| Language: | English |
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Ain Shams University
2018-06-01
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| Series: | Archives of Pharmaceutical Sciences Ain Shams University |
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| Online Access: | https://aps.journals.ekb.eg/article_18733.html |
| _version_ | 1830130607405924352 |
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| author | Raghda A. Singab Ahmed M. Elissawy Walid Faisal Elkhatib Mahmoud A. Yassien Nadia A. Hassouna |
| author_facet | Raghda A. Singab Ahmed M. Elissawy Walid Faisal Elkhatib Mahmoud A. Yassien Nadia A. Hassouna |
| author_sort | Raghda A. Singab |
| collection | DOAJ |
| description | In this study, a total number of 92 clinical isolates of Candida species was used to test their ability to transform caffeic acid using the two-stage fermentation protocol. The success of the biotransformation process was confirmed by TLC autography method and nuclear magnetic resonance analysis. The obtained chromatograms showed that 7 isolates could perform caffeic acid biotransformation. The biological activities (antibacterial, antifungal, antiviral, and cytotoxic activities) of the extracts of the selected isolates were determined. According to the obtained results, the Candida isolate CI-24 had the most promising biotransformation ability. The methanolic extract of the respective isolate showed a promising anticancer activity against Caco-2 cell line and a potential antibacterial activity against Staphylococcus aureus ATCC 25923. The isolate was genetically identified as Candida albicans (Accession number MH356583) using 28S rRNA sequencing. As determined by NMR and LC-MS analysis, caffeic acid was transformed by Candida albicans strain CI-24 into para-hydroxybenzoic acid. |
| first_indexed | 2024-12-17T06:00:36Z |
| format | Article |
| id | doaj.art-d414d84c0c844890961af5dacf40cc0e |
| institution | Directory Open Access Journal |
| issn | 2356-8380 2356-8399 |
| language | English |
| last_indexed | 2024-12-17T06:00:36Z |
| publishDate | 2018-06-01 |
| publisher | Ain Shams University |
| record_format | Article |
| series | Archives of Pharmaceutical Sciences Ain Shams University |
| spelling | doaj.art-d414d84c0c844890961af5dacf40cc0e2022-12-21T22:00:55ZengAin Shams UniversityArchives of Pharmaceutical Sciences Ain Shams University2356-83802356-83992018-06-01213746https://dx.doi.org/10.21608/aps.2018.18733Biotransformation of caffeic acid into a promising biologically active metabolite by Candida albicans isolate CI-24Raghda A. Singab0Ahmed M. Elissawy1Walid Faisal Elkhatib2Mahmoud A. Yassien3Nadia A. Hassouna4Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Cairo, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, Cairo, 11566, EgyptDepartment of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Cairo, EgyptDepartment of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Cairo, EgyptDepartment of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Cairo, EgyptIn this study, a total number of 92 clinical isolates of Candida species was used to test their ability to transform caffeic acid using the two-stage fermentation protocol. The success of the biotransformation process was confirmed by TLC autography method and nuclear magnetic resonance analysis. The obtained chromatograms showed that 7 isolates could perform caffeic acid biotransformation. The biological activities (antibacterial, antifungal, antiviral, and cytotoxic activities) of the extracts of the selected isolates were determined. According to the obtained results, the Candida isolate CI-24 had the most promising biotransformation ability. The methanolic extract of the respective isolate showed a promising anticancer activity against Caco-2 cell line and a potential antibacterial activity against Staphylococcus aureus ATCC 25923. The isolate was genetically identified as Candida albicans (Accession number MH356583) using 28S rRNA sequencing. As determined by NMR and LC-MS analysis, caffeic acid was transformed by Candida albicans strain CI-24 into para-hydroxybenzoic acid.https://aps.journals.ekb.eg/article_18733.htmlBiotransformationCaffeic acidCandida albicansantibacterialCytotoxicity |
| spellingShingle | Raghda A. Singab Ahmed M. Elissawy Walid Faisal Elkhatib Mahmoud A. Yassien Nadia A. Hassouna Biotransformation of caffeic acid into a promising biologically active metabolite by Candida albicans isolate CI-24 Archives of Pharmaceutical Sciences Ain Shams University Biotransformation Caffeic acid Candida albicans antibacterial Cytotoxicity |
| title | Biotransformation of caffeic acid into a promising biologically active metabolite by Candida albicans isolate CI-24 |
| title_full | Biotransformation of caffeic acid into a promising biologically active metabolite by Candida albicans isolate CI-24 |
| title_fullStr | Biotransformation of caffeic acid into a promising biologically active metabolite by Candida albicans isolate CI-24 |
| title_full_unstemmed | Biotransformation of caffeic acid into a promising biologically active metabolite by Candida albicans isolate CI-24 |
| title_short | Biotransformation of caffeic acid into a promising biologically active metabolite by Candida albicans isolate CI-24 |
| title_sort | biotransformation of caffeic acid into a promising biologically active metabolite by candida albicans isolate ci 24 |
| topic | Biotransformation Caffeic acid Candida albicans antibacterial Cytotoxicity |
| url | https://aps.journals.ekb.eg/article_18733.html |
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