A versatile, high-efficiency platform for CRISPR-based gene activation
The generation of CRISPR-mediated transcriptional activation (CRISPRa)-competent cell lines pose significant technical challenges. Here the authors report a platform for production of CRISPRa-ready cell populations which they combine with optimised expressed and synthetic gRNA scaffolds to enhance f...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-02-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-36452-w |
| _version_ | 1827983631900999680 |
|---|---|
| author | Amy J. Heidersbach Kristel M. Dorighi Javier A. Gomez Ashley M. Jacobi Benjamin Haley |
| author_facet | Amy J. Heidersbach Kristel M. Dorighi Javier A. Gomez Ashley M. Jacobi Benjamin Haley |
| author_sort | Amy J. Heidersbach |
| collection | DOAJ |
| description | The generation of CRISPR-mediated transcriptional activation (CRISPRa)-competent cell lines pose significant technical challenges. Here the authors report a platform for production of CRISPRa-ready cell populations which they combine with optimised expressed and synthetic gRNA scaffolds to enhance functionality. |
| first_indexed | 2024-04-09T22:49:50Z |
| format | Article |
| id | doaj.art-d41507a002324e478b140f579db08654 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-09T22:49:50Z |
| publishDate | 2023-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-d41507a002324e478b140f579db086542023-03-22T11:42:26ZengNature PortfolioNature Communications2041-17232023-02-0114111010.1038/s41467-023-36452-wA versatile, high-efficiency platform for CRISPR-based gene activationAmy J. Heidersbach0Kristel M. Dorighi1Javier A. Gomez2Ashley M. Jacobi3Benjamin Haley4Department of Molecular Biology, Genentech Inc.Department of Molecular Biology, Genentech Inc.Integrated DNA Technology IncIntegrated DNA Technology IncDepartment of Molecular Biology, Genentech Inc.The generation of CRISPR-mediated transcriptional activation (CRISPRa)-competent cell lines pose significant technical challenges. Here the authors report a platform for production of CRISPRa-ready cell populations which they combine with optimised expressed and synthetic gRNA scaffolds to enhance functionality.https://doi.org/10.1038/s41467-023-36452-w |
| spellingShingle | Amy J. Heidersbach Kristel M. Dorighi Javier A. Gomez Ashley M. Jacobi Benjamin Haley A versatile, high-efficiency platform for CRISPR-based gene activation Nature Communications |
| title | A versatile, high-efficiency platform for CRISPR-based gene activation |
| title_full | A versatile, high-efficiency platform for CRISPR-based gene activation |
| title_fullStr | A versatile, high-efficiency platform for CRISPR-based gene activation |
| title_full_unstemmed | A versatile, high-efficiency platform for CRISPR-based gene activation |
| title_short | A versatile, high-efficiency platform for CRISPR-based gene activation |
| title_sort | versatile high efficiency platform for crispr based gene activation |
| url | https://doi.org/10.1038/s41467-023-36452-w |
| work_keys_str_mv | AT amyjheidersbach aversatilehighefficiencyplatformforcrisprbasedgeneactivation AT kristelmdorighi aversatilehighefficiencyplatformforcrisprbasedgeneactivation AT javieragomez aversatilehighefficiencyplatformforcrisprbasedgeneactivation AT ashleymjacobi aversatilehighefficiencyplatformforcrisprbasedgeneactivation AT benjaminhaley aversatilehighefficiencyplatformforcrisprbasedgeneactivation AT amyjheidersbach versatilehighefficiencyplatformforcrisprbasedgeneactivation AT kristelmdorighi versatilehighefficiencyplatformforcrisprbasedgeneactivation AT javieragomez versatilehighefficiencyplatformforcrisprbasedgeneactivation AT ashleymjacobi versatilehighefficiencyplatformforcrisprbasedgeneactivation AT benjaminhaley versatilehighefficiencyplatformforcrisprbasedgeneactivation |