Chemical composition, antioxidant capacity and anti-inflammatory activity of the fruits of Mimusops coriacea (A.DC) Mig (Sapotaceae) that grows in Ecuador
Context: Mimusops coriacea (Sapotaceae) is traditionally used to treat inflammation of the urethra, cystitis, diarrhea, and dysentery, among others. In Ecuador it is used as an analgesic and anti-inflammatory, however the phytochemical and pharmacological information is scarce. Aims: To evaluate...
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| Format: | Article |
| Language: | English |
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GarVal Editorial Ltda.
2021-01-01
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| Series: | Journal of Pharmacy & Pharmacognosy Research |
| Subjects: | |
| Online Access: | http://jppres.com/jppres/pdf/vol9/jppres20.917_9.1.33.pdf |
| _version_ | 1811313630878105600 |
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| author | Katherine E. Bustamante Pesantes Yamilet I. Gutiérrez Gaitén Iván A. Chóez Guaranda Migdalia Miranda Martínez |
| author_facet | Katherine E. Bustamante Pesantes Yamilet I. Gutiérrez Gaitén Iván A. Chóez Guaranda Migdalia Miranda Martínez |
| author_sort | Katherine E. Bustamante Pesantes |
| collection | DOAJ |
| description | Context: Mimusops coriacea (Sapotaceae) is traditionally used to treat inflammation of the urethra, cystitis, diarrhea, and dysentery, among others. In Ecuador it is used as an analgesic and anti-inflammatory, however the phytochemical and pharmacological information is scarce.
Aims: To evaluate the chemical composition, antioxidant capacity and anti-inflammatory activity of the M. coriacea fruits in two stages of ripening.
Methods: An extraction with hexane by Soxhlet of the oils of the seeds of green and mature fruits was carried out. Saponifiable and unsaponifiable fractions were analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). Hydroalcoholic extracts were obtained by maceration from the green and ripe whole fruits and were tested by Liquid Chromatography-Mass Spectrometry (LC-MS). Antioxidant capacity by FRAP, DPPH and ABTS and anti-inflammatory activity using the plantar edema model by carrageenan were evaluated.
Results: In the saponifiable fractions, four fatty acids were identified (majority 9-octadecenoic acid) and in the unsaponifiable ones four compounds were assigned to green fruits and nine to mature fruits, the majority being squalene and urs-12-en-24-oico, 3-oxo-methyl ester, respectively. By LC-MS 31 compounds were identified, associated with phenolic compounds and triterpenic saponins. The extracts showed Fe3+ reducing capacity and antiradical activity, the green fruit extract was the most active (IC50s = 4.99 μg/mL and 246.80 μg/mL, DPPH and ABTS, respectively). The anti-inflammatory activity of the extracts was demonstrated with inhibition percentages greater than 50% by using indomethacin as reference drug.
Conclusions: The results provide the first findings of the phytochemical and bioactive potential of the M. coriacea species that grows in Ecuador. |
| first_indexed | 2024-04-13T10:57:01Z |
| format | Article |
| id | doaj.art-d41713fd72974bfb91f8590394e95cc1 |
| institution | Directory Open Access Journal |
| issn | 0719-4250 |
| language | English |
| last_indexed | 2024-04-13T10:57:01Z |
| publishDate | 2021-01-01 |
| publisher | GarVal Editorial Ltda. |
| record_format | Article |
| series | Journal of Pharmacy & Pharmacognosy Research |
| spelling | doaj.art-d41713fd72974bfb91f8590394e95cc12022-12-22T02:49:30ZengGarVal Editorial Ltda.Journal of Pharmacy & Pharmacognosy Research0719-42502021-01-01913348Chemical composition, antioxidant capacity and anti-inflammatory activity of the fruits of Mimusops coriacea (A.DC) Mig (Sapotaceae) that grows in EcuadorKatherine E. Bustamante Pesantes0Yamilet I. Gutiérrez Gaitén1Iván A. Chóez Guaranda2Migdalia Miranda Martínez3Departamento de Farmacia. Facultad de Ciencias Químicas. Universidad de Guayaquil. Ciudadela Universitaria “Salvador Allende”. Ave. Kennedy S/N y Av. Delta. Guayaquil. Ecuador.Departamento de Farmacia, Instituto de Farmacia y Alimentos, Universidad de La Habana, Coronela, Lisa, Habana 13600, Cuba.Centro de Investigaciones Biotecnológicas del Ecuador. Escuela Superior Politécnica del Litoral, ESPOL. Campus Gustavo Galindo Km. 30.5 Vía Perimetral, P.O. Box 09-01-5863. Guayaquil, Ecuador.Centro de Investigaciones Biotecnológicas del Ecuador. Escuela Superior Politécnica del Litoral, ESPOL. Campus Gustavo Galindo Km. 30.5 Vía Perimetral, P.O. Box 09-01-5863. Guayaquil, Ecuador. Departamento de Ciencias Químicas y Ambientales. Facultad de Ciencias Naturales y Matemáticas. Escuela Superior Politécnica del Litoral, ESPOL. Campus Gustavo Galindo Km. 30.5 Vía Perimetral, P.O. Box 09-01-5863. Guayaquil, Ecuador.Context: Mimusops coriacea (Sapotaceae) is traditionally used to treat inflammation of the urethra, cystitis, diarrhea, and dysentery, among others. In Ecuador it is used as an analgesic and anti-inflammatory, however the phytochemical and pharmacological information is scarce. Aims: To evaluate the chemical composition, antioxidant capacity and anti-inflammatory activity of the M. coriacea fruits in two stages of ripening. Methods: An extraction with hexane by Soxhlet of the oils of the seeds of green and mature fruits was carried out. Saponifiable and unsaponifiable fractions were analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). Hydroalcoholic extracts were obtained by maceration from the green and ripe whole fruits and were tested by Liquid Chromatography-Mass Spectrometry (LC-MS). Antioxidant capacity by FRAP, DPPH and ABTS and anti-inflammatory activity using the plantar edema model by carrageenan were evaluated. Results: In the saponifiable fractions, four fatty acids were identified (majority 9-octadecenoic acid) and in the unsaponifiable ones four compounds were assigned to green fruits and nine to mature fruits, the majority being squalene and urs-12-en-24-oico, 3-oxo-methyl ester, respectively. By LC-MS 31 compounds were identified, associated with phenolic compounds and triterpenic saponins. The extracts showed Fe3+ reducing capacity and antiradical activity, the green fruit extract was the most active (IC50s = 4.99 μg/mL and 246.80 μg/mL, DPPH and ABTS, respectively). The anti-inflammatory activity of the extracts was demonstrated with inhibition percentages greater than 50% by using indomethacin as reference drug. Conclusions: The results provide the first findings of the phytochemical and bioactive potential of the M. coriacea species that grows in Ecuador.http://jppres.com/jppres/pdf/vol9/jppres20.917_9.1.33.pdfanti-inflammatory activityantioxidant activitygc-mslc-msmimusops coriaceafruit |
| spellingShingle | Katherine E. Bustamante Pesantes Yamilet I. Gutiérrez Gaitén Iván A. Chóez Guaranda Migdalia Miranda Martínez Chemical composition, antioxidant capacity and anti-inflammatory activity of the fruits of Mimusops coriacea (A.DC) Mig (Sapotaceae) that grows in Ecuador Journal of Pharmacy & Pharmacognosy Research anti-inflammatory activity antioxidant activity gc-ms lc-ms mimusops coriacea fruit |
| title | Chemical composition, antioxidant capacity and anti-inflammatory activity of the fruits of Mimusops coriacea (A.DC) Mig (Sapotaceae) that grows in Ecuador |
| title_full | Chemical composition, antioxidant capacity and anti-inflammatory activity of the fruits of Mimusops coriacea (A.DC) Mig (Sapotaceae) that grows in Ecuador |
| title_fullStr | Chemical composition, antioxidant capacity and anti-inflammatory activity of the fruits of Mimusops coriacea (A.DC) Mig (Sapotaceae) that grows in Ecuador |
| title_full_unstemmed | Chemical composition, antioxidant capacity and anti-inflammatory activity of the fruits of Mimusops coriacea (A.DC) Mig (Sapotaceae) that grows in Ecuador |
| title_short | Chemical composition, antioxidant capacity and anti-inflammatory activity of the fruits of Mimusops coriacea (A.DC) Mig (Sapotaceae) that grows in Ecuador |
| title_sort | chemical composition antioxidant capacity and anti inflammatory activity of the fruits of mimusops coriacea a dc mig sapotaceae that grows in ecuador |
| topic | anti-inflammatory activity antioxidant activity gc-ms lc-ms mimusops coriacea fruit |
| url | http://jppres.com/jppres/pdf/vol9/jppres20.917_9.1.33.pdf |
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