Generation of a genetically-modified induced pluripotent stem cell line harboring an oncogenic gene variant KRAS p.G12V
Activating KRAS codon 12 gene variants are known to cause severe RAS-MAPK and PI3K-AKT signaling pathway hyperactivity and are frequently involved in the development of various carcinomas. Here, we describe the generation of a human iPSC line harboring the common oncogenic KRAS p.G12V variant by usi...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-06-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506123000910 |
| _version_ | 1797816008487993344 |
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| author | Alexandra Viktoria Busley Mandy Kleinsorge Lukas Cyganek |
| author_facet | Alexandra Viktoria Busley Mandy Kleinsorge Lukas Cyganek |
| author_sort | Alexandra Viktoria Busley |
| collection | DOAJ |
| description | Activating KRAS codon 12 gene variants are known to cause severe RAS-MAPK and PI3K-AKT signaling pathway hyperactivity and are frequently involved in the development of various carcinomas. Here, we describe the generation of a human iPSC line harboring the common oncogenic KRAS p.G12V variant by using CRISPR/Cas9 technology. The established KRASG12V iPSC line allows the study of oncogenic KRAS-induced signaling dysregulation and its impact on cell physiology in various iPSC-derived cell types and tissues. Furthermore, it might serve as a powerful platform for drug and toxicity screenings to identify new chemotherapeutic drugs. |
| first_indexed | 2024-03-13T08:31:18Z |
| format | Article |
| id | doaj.art-d41ae05f63d64ff78da7c1f5e75123a9 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-03-13T08:31:18Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-d41ae05f63d64ff78da7c1f5e75123a92023-05-31T04:44:13ZengElsevierStem Cell Research1873-50612023-06-0169103105Generation of a genetically-modified induced pluripotent stem cell line harboring an oncogenic gene variant KRAS p.G12VAlexandra Viktoria Busley0Mandy Kleinsorge1Lukas Cyganek2Stem Cell Unit, Clinic for Cardiology and Pneumology, University Medical Center Göttingen, Göttingen, Germany; DZHK (German Center for Cardiovascular Research), Partner Site Göttingen, Germany; Cluster of Excellence “Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells” (MBExC), University of Göttingen, Germany & Hertha Sponer College, Göttingen, GermanyStem Cell Unit, Clinic for Cardiology and Pneumology, University Medical Center Göttingen, Göttingen, Germany; Institute of Health in der Charité (BIH), Center of Biological Design, Berlin, GermanyStem Cell Unit, Clinic for Cardiology and Pneumology, University Medical Center Göttingen, Göttingen, Germany; DZHK (German Center for Cardiovascular Research), Partner Site Göttingen, Germany; Cluster of Excellence “Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells” (MBExC), University of Göttingen, Germany & Hertha Sponer College, Göttingen, Germany; Corresponding author.Activating KRAS codon 12 gene variants are known to cause severe RAS-MAPK and PI3K-AKT signaling pathway hyperactivity and are frequently involved in the development of various carcinomas. Here, we describe the generation of a human iPSC line harboring the common oncogenic KRAS p.G12V variant by using CRISPR/Cas9 technology. The established KRASG12V iPSC line allows the study of oncogenic KRAS-induced signaling dysregulation and its impact on cell physiology in various iPSC-derived cell types and tissues. Furthermore, it might serve as a powerful platform for drug and toxicity screenings to identify new chemotherapeutic drugs.http://www.sciencedirect.com/science/article/pii/S1873506123000910 |
| spellingShingle | Alexandra Viktoria Busley Mandy Kleinsorge Lukas Cyganek Generation of a genetically-modified induced pluripotent stem cell line harboring an oncogenic gene variant KRAS p.G12V Stem Cell Research |
| title | Generation of a genetically-modified induced pluripotent stem cell line harboring an oncogenic gene variant KRAS p.G12V |
| title_full | Generation of a genetically-modified induced pluripotent stem cell line harboring an oncogenic gene variant KRAS p.G12V |
| title_fullStr | Generation of a genetically-modified induced pluripotent stem cell line harboring an oncogenic gene variant KRAS p.G12V |
| title_full_unstemmed | Generation of a genetically-modified induced pluripotent stem cell line harboring an oncogenic gene variant KRAS p.G12V |
| title_short | Generation of a genetically-modified induced pluripotent stem cell line harboring an oncogenic gene variant KRAS p.G12V |
| title_sort | generation of a genetically modified induced pluripotent stem cell line harboring an oncogenic gene variant kras p g12v |
| url | http://www.sciencedirect.com/science/article/pii/S1873506123000910 |
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