Proteome changes of sheep rumen epithelium during postnatal development
Background: The development of the rumen epithelium is a critical physiological challenge for sheep. However, the molecular mechanism underlying postnatal rumen development in sheep remains rarely understood.Results: Here, we used a shotgun approach and bioinformatics analyses to investigate and com...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2022.1031707/full |
| _version_ | 1811338832197451776 |
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| author | Kaizhi Zheng Liangyong Guo Saif Ullah Yang Cao Xin Huang Huili shan Junfang Jiang Jianliang Wu Yongqing Jiang |
| author_facet | Kaizhi Zheng Liangyong Guo Saif Ullah Yang Cao Xin Huang Huili shan Junfang Jiang Jianliang Wu Yongqing Jiang |
| author_sort | Kaizhi Zheng |
| collection | DOAJ |
| description | Background: The development of the rumen epithelium is a critical physiological challenge for sheep. However, the molecular mechanism underlying postnatal rumen development in sheep remains rarely understood.Results: Here, we used a shotgun approach and bioinformatics analyses to investigate and compare proteomic profiles of sheep rumen epithelium tissue on day 0, 15, 30, 45, and 60 of age. A total of 4,523 proteins were identified, in which we found 852, 342, 164, and 95 differentially expressed proteins (DEPs) between day 0 and day 15, between day 15 and day 30, between day 30 and day 45, between day 45 and day 60, respectively. Furthermore, subcellular localization analysis showed that the DEPs were majorly localized in mitochondrion between day 0 and day 15, after which nucleus proteins were the most DEPs. Finally, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that DEPs significantly enriched in mitochondrion, ubiquitination, histone modifications, glutathione synthase activity, and wnt and nortch signaling pathways.Conclusion: Our data indicate that the biogenesis of mitochondrion in rumen epithelial cell is essential for the initiation of rumen epithelial development. Glutathione, wnt signaling pathway and nortch signaling pathway participated in rumen epithelial growth. Ubiquitination, post-translational modifications of histone might be key molecular functions in regulating rumen epithelial development. |
| first_indexed | 2024-04-13T18:16:26Z |
| format | Article |
| id | doaj.art-d41b27e974db41c38da03ef2e5ef69c7 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-04-13T18:16:26Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-d41b27e974db41c38da03ef2e5ef69c72022-12-22T02:35:39ZengFrontiers Media S.A.Frontiers in Genetics1664-80212022-10-011310.3389/fgene.2022.10317071031707Proteome changes of sheep rumen epithelium during postnatal developmentKaizhi Zheng0Liangyong Guo1Saif Ullah2Yang Cao3Xin Huang4Huili shan5Junfang Jiang6Jianliang Wu7Yongqing Jiang8Institute of Animal Husbandry and Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaHuzhou Academy of Agricultural Sciences, Huzhou, ChinaFaculty of Veterinary and Animal Sciences, Lasbela University of Agriculture Water and Marine Sciences, Lasbela, PakistanInstitute of Animal Husbandry and Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaInstitute of Animal Husbandry and Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaInstitute of Animal Husbandry and Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaInstitute of Animal Husbandry and Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaInstitute of Animal Husbandry and Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaInstitute of Animal Husbandry and Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaBackground: The development of the rumen epithelium is a critical physiological challenge for sheep. However, the molecular mechanism underlying postnatal rumen development in sheep remains rarely understood.Results: Here, we used a shotgun approach and bioinformatics analyses to investigate and compare proteomic profiles of sheep rumen epithelium tissue on day 0, 15, 30, 45, and 60 of age. A total of 4,523 proteins were identified, in which we found 852, 342, 164, and 95 differentially expressed proteins (DEPs) between day 0 and day 15, between day 15 and day 30, between day 30 and day 45, between day 45 and day 60, respectively. Furthermore, subcellular localization analysis showed that the DEPs were majorly localized in mitochondrion between day 0 and day 15, after which nucleus proteins were the most DEPs. Finally, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that DEPs significantly enriched in mitochondrion, ubiquitination, histone modifications, glutathione synthase activity, and wnt and nortch signaling pathways.Conclusion: Our data indicate that the biogenesis of mitochondrion in rumen epithelial cell is essential for the initiation of rumen epithelial development. Glutathione, wnt signaling pathway and nortch signaling pathway participated in rumen epithelial growth. Ubiquitination, post-translational modifications of histone might be key molecular functions in regulating rumen epithelial development.https://www.frontiersin.org/articles/10.3389/fgene.2022.1031707/fullproteomesheeprumenepitheliumpostnatal development |
| spellingShingle | Kaizhi Zheng Liangyong Guo Saif Ullah Yang Cao Xin Huang Huili shan Junfang Jiang Jianliang Wu Yongqing Jiang Proteome changes of sheep rumen epithelium during postnatal development Frontiers in Genetics proteome sheep rumen epithelium postnatal development |
| title | Proteome changes of sheep rumen epithelium during postnatal development |
| title_full | Proteome changes of sheep rumen epithelium during postnatal development |
| title_fullStr | Proteome changes of sheep rumen epithelium during postnatal development |
| title_full_unstemmed | Proteome changes of sheep rumen epithelium during postnatal development |
| title_short | Proteome changes of sheep rumen epithelium during postnatal development |
| title_sort | proteome changes of sheep rumen epithelium during postnatal development |
| topic | proteome sheep rumen epithelium postnatal development |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2022.1031707/full |
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