MiR‐451a ameliorates alcoholic hepatitis via repressing HDAC8‐mediated proinflammatory response
Abstract Alcoholic hepatitis (AH) is identified as an inflammatory syndrome with high morbidity and mortality as a result of severe hepatocellular dysfunction and liver injury. Accumulated studies indicated that miRNAs are involved in AH. The potential effect of miR‐451a in AH mice was examined in t...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-11-01
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| Series: | Kaohsiung Journal of Medical Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/kjm2.12272 |
| _version_ | 1819172972353552384 |
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| author | Bo Du Xiao‐Hong Tan Ling Cheng Feng Wang Hai‐Feng Zhang |
| author_facet | Bo Du Xiao‐Hong Tan Ling Cheng Feng Wang Hai‐Feng Zhang |
| author_sort | Bo Du |
| collection | DOAJ |
| description | Abstract Alcoholic hepatitis (AH) is identified as an inflammatory syndrome with high morbidity and mortality as a result of severe hepatocellular dysfunction and liver injury. Accumulated studies indicated that miRNAs are involved in AH. The potential effect of miR‐451a in AH mice was examined in the current study. A mice AH model was established and the miR‐451a expression in AH mice compared with the sham group was tested by real‐time polymerase chain reaction (qRT‐PCR). AH mice were injected with pre‐miR‐451a lentivirus for miR‐451a overexpression and histone deacetylase (HDAC8) lentivirus for HDAC8 overexpression in AH mice. The underlying mechanisms were explored by searching the potential target genes of miR‐451a in miRanda database and then we confirmed this. We found that miR‐451a expression was significantly decreased in AH mice compared with the sham group. Moreover, miR‐451a overexpression alleviated alcohol‐induced liver inflammation and injuries of AH mice. Additionally, further mechanism exploration disclosed that HDAC8 was a target of miR‐451a. The protective effect of miR‐451a on AH in AH mice was abolished by HDAC8 overexpression. In summary, miR‐451a ameliorates AH via repressing HDAC8‐mediated proinflammatory response. |
| first_indexed | 2024-12-22T20:15:40Z |
| format | Article |
| id | doaj.art-d41bf40adfc4421cb874156c42382436 |
| institution | Directory Open Access Journal |
| issn | 1607-551X 2410-8650 |
| language | English |
| last_indexed | 2024-12-22T20:15:40Z |
| publishDate | 2020-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Kaohsiung Journal of Medical Sciences |
| spelling | doaj.art-d41bf40adfc4421cb874156c423824362022-12-21T18:13:58ZengWileyKaohsiung Journal of Medical Sciences1607-551X2410-86502020-11-01361190491010.1002/kjm2.12272MiR‐451a ameliorates alcoholic hepatitis via repressing HDAC8‐mediated proinflammatory responseBo Du0Xiao‐Hong Tan1Ling Cheng2Feng Wang3Hai‐Feng Zhang4Department of Hepatobiliary Surgery The People's Hospital of Kaizhou District Chongqing ChinaDepartment of Hepatobiliary Surgery The People's Hospital of Kaizhou District Chongqing ChinaDepartment of Hepatobiliary Surgery The People's Hospital of Kaizhou District Chongqing ChinaDepartment of Physiology Inner Mongolia Medical University Hohhot Inner Mongolia Autonomous Region ChinaDepartment of Physiology Inner Mongolia Medical University Hohhot Inner Mongolia Autonomous Region ChinaAbstract Alcoholic hepatitis (AH) is identified as an inflammatory syndrome with high morbidity and mortality as a result of severe hepatocellular dysfunction and liver injury. Accumulated studies indicated that miRNAs are involved in AH. The potential effect of miR‐451a in AH mice was examined in the current study. A mice AH model was established and the miR‐451a expression in AH mice compared with the sham group was tested by real‐time polymerase chain reaction (qRT‐PCR). AH mice were injected with pre‐miR‐451a lentivirus for miR‐451a overexpression and histone deacetylase (HDAC8) lentivirus for HDAC8 overexpression in AH mice. The underlying mechanisms were explored by searching the potential target genes of miR‐451a in miRanda database and then we confirmed this. We found that miR‐451a expression was significantly decreased in AH mice compared with the sham group. Moreover, miR‐451a overexpression alleviated alcohol‐induced liver inflammation and injuries of AH mice. Additionally, further mechanism exploration disclosed that HDAC8 was a target of miR‐451a. The protective effect of miR‐451a on AH in AH mice was abolished by HDAC8 overexpression. In summary, miR‐451a ameliorates AH via repressing HDAC8‐mediated proinflammatory response.https://doi.org/10.1002/kjm2.12272alcoholic hepatitisHDAC8inflammationmiR‐451a |
| spellingShingle | Bo Du Xiao‐Hong Tan Ling Cheng Feng Wang Hai‐Feng Zhang MiR‐451a ameliorates alcoholic hepatitis via repressing HDAC8‐mediated proinflammatory response Kaohsiung Journal of Medical Sciences alcoholic hepatitis HDAC8 inflammation miR‐451a |
| title | MiR‐451a ameliorates alcoholic hepatitis via repressing HDAC8‐mediated proinflammatory response |
| title_full | MiR‐451a ameliorates alcoholic hepatitis via repressing HDAC8‐mediated proinflammatory response |
| title_fullStr | MiR‐451a ameliorates alcoholic hepatitis via repressing HDAC8‐mediated proinflammatory response |
| title_full_unstemmed | MiR‐451a ameliorates alcoholic hepatitis via repressing HDAC8‐mediated proinflammatory response |
| title_short | MiR‐451a ameliorates alcoholic hepatitis via repressing HDAC8‐mediated proinflammatory response |
| title_sort | mir 451a ameliorates alcoholic hepatitis via repressing hdac8 mediated proinflammatory response |
| topic | alcoholic hepatitis HDAC8 inflammation miR‐451a |
| url | https://doi.org/10.1002/kjm2.12272 |
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