Evolutionary study of potentially zoonotic hepatitis E virus genotype 3 from swine in Northeast Brazil
Hepatitis E virus (HEV), an emerging virus associated with acute hepatic disease, leads to thousands of deaths worldwide. HEV has already been reported in Brazil; however, there is a lack of epidemiological and molecular information on the genetic variability, taxonomy, and evolution of HEV. It is t...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762019000100410&lng=en&tlng=en |
| _version_ | 1797702933833318400 |
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| author | Edmilson Ferreira de Oliveira-Filho Debora RL dos Santos Ricardo Durães-Carvalho Adalúcia da Silva Gustavo Barbosa de Lima Antônio Fernando B Batista Filho Lindomar J Pena Laura HVG Gil |
| author_facet | Edmilson Ferreira de Oliveira-Filho Debora RL dos Santos Ricardo Durães-Carvalho Adalúcia da Silva Gustavo Barbosa de Lima Antônio Fernando B Batista Filho Lindomar J Pena Laura HVG Gil |
| author_sort | Edmilson Ferreira de Oliveira-Filho |
| collection | DOAJ |
| description | Hepatitis E virus (HEV), an emerging virus associated with acute hepatic disease, leads to thousands of deaths worldwide. HEV has already been reported in Brazil; however, there is a lack of epidemiological and molecular information on the genetic variability, taxonomy, and evolution of HEV. It is thus unclear whether hepatitis E is a neglected disease in Brazil or it has low relevance for public health in this country. Here, for the first time, we report the presence of HEV in Northeast Brazil. A total of 119 swine faecal samples were screened for the presence of HEV RNA using real-time polymerase chain reaction (RT-PCR) and further confirmed by conventional RT-PCR; among these, two samples were identified as positive. Molecular evolution analyses based on capsid sequences revealed that the samples had close proximities to HEV sequences belonging to genotype 3 and were genetically related to subtype 3f isolated in humans. Parsimony ancestral states analysis indicated gene flow events from HEV cross-species infection, suggesting an important role of pig hosts in viral spillover. HEV’s ability for zoonotic transmission by inter-species host switching as well as its possible adaptation to new animal species remain important issues for human health. |
| first_indexed | 2024-03-12T04:58:06Z |
| format | Article |
| id | doaj.art-d41d9a09081440269dc09ddad4688be4 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T04:58:06Z |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-d41d9a09081440269dc09ddad4688be42023-09-03T09:14:43ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-8060114010.1590/0074-02760180585S0074-02762019000100410Evolutionary study of potentially zoonotic hepatitis E virus genotype 3 from swine in Northeast BrazilEdmilson Ferreira de Oliveira-FilhoDebora RL dos SantosRicardo Durães-CarvalhoAdalúcia da SilvaGustavo Barbosa de LimaAntônio Fernando B Batista FilhoLindomar J PenaLaura HVG GilHepatitis E virus (HEV), an emerging virus associated with acute hepatic disease, leads to thousands of deaths worldwide. HEV has already been reported in Brazil; however, there is a lack of epidemiological and molecular information on the genetic variability, taxonomy, and evolution of HEV. It is thus unclear whether hepatitis E is a neglected disease in Brazil or it has low relevance for public health in this country. Here, for the first time, we report the presence of HEV in Northeast Brazil. A total of 119 swine faecal samples were screened for the presence of HEV RNA using real-time polymerase chain reaction (RT-PCR) and further confirmed by conventional RT-PCR; among these, two samples were identified as positive. Molecular evolution analyses based on capsid sequences revealed that the samples had close proximities to HEV sequences belonging to genotype 3 and were genetically related to subtype 3f isolated in humans. Parsimony ancestral states analysis indicated gene flow events from HEV cross-species infection, suggesting an important role of pig hosts in viral spillover. HEV’s ability for zoonotic transmission by inter-species host switching as well as its possible adaptation to new animal species remain important issues for human health.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762019000100410&lng=en&tlng=enzoonotic hepatitis E virusswinegenotype 3 |
| spellingShingle | Edmilson Ferreira de Oliveira-Filho Debora RL dos Santos Ricardo Durães-Carvalho Adalúcia da Silva Gustavo Barbosa de Lima Antônio Fernando B Batista Filho Lindomar J Pena Laura HVG Gil Evolutionary study of potentially zoonotic hepatitis E virus genotype 3 from swine in Northeast Brazil Memorias do Instituto Oswaldo Cruz zoonotic hepatitis E virus swine genotype 3 |
| title | Evolutionary study of potentially zoonotic hepatitis E virus genotype 3 from swine in Northeast Brazil |
| title_full | Evolutionary study of potentially zoonotic hepatitis E virus genotype 3 from swine in Northeast Brazil |
| title_fullStr | Evolutionary study of potentially zoonotic hepatitis E virus genotype 3 from swine in Northeast Brazil |
| title_full_unstemmed | Evolutionary study of potentially zoonotic hepatitis E virus genotype 3 from swine in Northeast Brazil |
| title_short | Evolutionary study of potentially zoonotic hepatitis E virus genotype 3 from swine in Northeast Brazil |
| title_sort | evolutionary study of potentially zoonotic hepatitis e virus genotype 3 from swine in northeast brazil |
| topic | zoonotic hepatitis E virus swine genotype 3 |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762019000100410&lng=en&tlng=en |
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