Association of Human Leukocyte AntigenCw6 in Psoriasis Patients with Disease Severity and Morphological Patterns: A Cross-control Study in a Tertiary Care Referral Centre in Eastern India
Introduction: Psoriasis is a multifactorial disorder in which genetic and environmental factors play an essential role in disease pathogenesis. The Human Leukocyte Antigen-Cw6 (HLA-Cw6) allele has shown the strongest genetic association with this condition across several populations studied. Ai...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2021-08-01
|
| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/15198/50595_CE[Ra1]_F[SK]_PF1(AG_OM)_PFA(KM)_PB(AG_KM)_PN(KM).pdf |
| _version_ | 1823964387882303488 |
|---|---|
| author | Akhilesh Behra Gobinda Chatterjee Aditi Chandra Raghunath Chatterjee |
| author_facet | Akhilesh Behra Gobinda Chatterjee Aditi Chandra Raghunath Chatterjee |
| author_sort | Akhilesh Behra |
| collection | DOAJ |
| description | Introduction: Psoriasis is a multifactorial disorder in which
genetic and environmental factors play an essential role in
disease pathogenesis. The Human Leukocyte Antigen-Cw6
(HLA-Cw6) allele has shown the strongest genetic association
with this condition across several populations studied.
Aim: To estimate the risk of HLA-Cw6 association with psoriasis
compared to the control group and its association with the
severity of psoriasis expressed as Psoriasis Area and Severity
Index (PASI) score and morphological patterns.
Materials and Methods: A cross-control study was conducted
from March 2014 to February 2015 in a tertiary care centre
in the eastern part of India. All patients who were diagnosed
clinically as psoriasis and gave written informed consent were
included in the study. Healthy controls were taken to compare
the HLA-Cw6 after duly signing the informed consent for
detailed history, clinical examination, PASI score, and digital
photographs of lesions were taken. A blood sample was taken
from patients and the control for HLA-Cw6 typing by sequencespecific Polymerase Chain Reaction (PCR) method. Data was
analysed by appropriate statistical test (Chi-square test) using
R statistical software.
Results: A total of 100 patients with psoriasis and 100 controls
were recruited, of which 61 were positive for HLA-Cw6 among
the psoriasis group and nine in the control group. Positivity of
the HLA-Cw6 allele was significantly higher in psoriasis cases
(n=61) compared to normal individuals (n=9) (p-value: 1.79×10-15,
OR: 15.8148, 95% CI: 7.15-34.99). It was also observed that
HLA-Cw6 positive individuals had a more severe form of the
disease, determined by a PASI score >6 (p-value: 0.0494, OR:
2.22, 95% CI: 0.96-5.15), and significant involvement of scalp
psoriasis (p-value: 0.0054, OR: 13.125, 95% CI: 1.55-111.42).
However, no significant association of HLA-Cw6 was seen with
positive family history, nail involvement, and joint pain (arthralgia).
Conclusion: The HLA-Cw6 positivity was associated with a
more severe form of disease and scalp psoriasis. |
| first_indexed | 2024-12-17T17:42:48Z |
| format | Article |
| id | doaj.art-d41e9ef079f24a63983e0866c35e6436 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-17T17:42:48Z |
| publishDate | 2021-08-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-d41e9ef079f24a63983e0866c35e64362022-12-21T21:39:08ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2021-08-01158WC01WC0310.7860/JCDR/2021/50595.15198Association of Human Leukocyte AntigenCw6 in Psoriasis Patients with Disease Severity and Morphological Patterns: A Cross-control Study in a Tertiary Care Referral Centre in Eastern IndiaAkhilesh Behra0Gobinda Chatterjee1Aditi Chandra2Raghunath Chatterjee3Assistant Professor, Department of Dermatology, LSLRAM Medical College, Raigarh, Chhattisgarh, India.Professor, Department of Dermatology, IPGME&R and SSKM Hospital, Kolkata, West Bengal, India.Post Doctoral Fellow, Perelman School of Medicine, University of Pennsylvania, Philadelphia, United States of America.Associate Professor, Department of Human Genetics Unit, Indian Statistical Institute, Kolkata, West Bengal, India.Introduction: Psoriasis is a multifactorial disorder in which genetic and environmental factors play an essential role in disease pathogenesis. The Human Leukocyte Antigen-Cw6 (HLA-Cw6) allele has shown the strongest genetic association with this condition across several populations studied. Aim: To estimate the risk of HLA-Cw6 association with psoriasis compared to the control group and its association with the severity of psoriasis expressed as Psoriasis Area and Severity Index (PASI) score and morphological patterns. Materials and Methods: A cross-control study was conducted from March 2014 to February 2015 in a tertiary care centre in the eastern part of India. All patients who were diagnosed clinically as psoriasis and gave written informed consent were included in the study. Healthy controls were taken to compare the HLA-Cw6 after duly signing the informed consent for detailed history, clinical examination, PASI score, and digital photographs of lesions were taken. A blood sample was taken from patients and the control for HLA-Cw6 typing by sequencespecific Polymerase Chain Reaction (PCR) method. Data was analysed by appropriate statistical test (Chi-square test) using R statistical software. Results: A total of 100 patients with psoriasis and 100 controls were recruited, of which 61 were positive for HLA-Cw6 among the psoriasis group and nine in the control group. Positivity of the HLA-Cw6 allele was significantly higher in psoriasis cases (n=61) compared to normal individuals (n=9) (p-value: 1.79×10-15, OR: 15.8148, 95% CI: 7.15-34.99). It was also observed that HLA-Cw6 positive individuals had a more severe form of the disease, determined by a PASI score >6 (p-value: 0.0494, OR: 2.22, 95% CI: 0.96-5.15), and significant involvement of scalp psoriasis (p-value: 0.0054, OR: 13.125, 95% CI: 1.55-111.42). However, no significant association of HLA-Cw6 was seen with positive family history, nail involvement, and joint pain (arthralgia). Conclusion: The HLA-Cw6 positivity was associated with a more severe form of disease and scalp psoriasis.https://jcdr.net/articles/PDF/15198/50595_CE[Ra1]_F[SK]_PF1(AG_OM)_PFA(KM)_PB(AG_KM)_PN(KM).pdfmorphological patternsnail involvementpolymerase chain reactionpsoriasis area and severity index |
| spellingShingle | Akhilesh Behra Gobinda Chatterjee Aditi Chandra Raghunath Chatterjee Association of Human Leukocyte AntigenCw6 in Psoriasis Patients with Disease Severity and Morphological Patterns: A Cross-control Study in a Tertiary Care Referral Centre in Eastern India Journal of Clinical and Diagnostic Research morphological patterns nail involvement polymerase chain reaction psoriasis area and severity index |
| title | Association of Human Leukocyte AntigenCw6 in Psoriasis Patients with Disease Severity and Morphological Patterns: A Cross-control Study in a Tertiary Care Referral Centre in Eastern India |
| title_full | Association of Human Leukocyte AntigenCw6 in Psoriasis Patients with Disease Severity and Morphological Patterns: A Cross-control Study in a Tertiary Care Referral Centre in Eastern India |
| title_fullStr | Association of Human Leukocyte AntigenCw6 in Psoriasis Patients with Disease Severity and Morphological Patterns: A Cross-control Study in a Tertiary Care Referral Centre in Eastern India |
| title_full_unstemmed | Association of Human Leukocyte AntigenCw6 in Psoriasis Patients with Disease Severity and Morphological Patterns: A Cross-control Study in a Tertiary Care Referral Centre in Eastern India |
| title_short | Association of Human Leukocyte AntigenCw6 in Psoriasis Patients with Disease Severity and Morphological Patterns: A Cross-control Study in a Tertiary Care Referral Centre in Eastern India |
| title_sort | association of human leukocyte antigencw6 in psoriasis patients with disease severity and morphological patterns a cross control study in a tertiary care referral centre in eastern india |
| topic | morphological patterns nail involvement polymerase chain reaction psoriasis area and severity index |
| url | https://jcdr.net/articles/PDF/15198/50595_CE[Ra1]_F[SK]_PF1(AG_OM)_PFA(KM)_PB(AG_KM)_PN(KM).pdf |
| work_keys_str_mv | AT akhileshbehra associationofhumanleukocyteantigencw6inpsoriasispatientswithdiseaseseverityandmorphologicalpatternsacrosscontrolstudyinatertiarycarereferralcentreineasternindia AT gobindachatterjee associationofhumanleukocyteantigencw6inpsoriasispatientswithdiseaseseverityandmorphologicalpatternsacrosscontrolstudyinatertiarycarereferralcentreineasternindia AT aditichandra associationofhumanleukocyteantigencw6inpsoriasispatientswithdiseaseseverityandmorphologicalpatternsacrosscontrolstudyinatertiarycarereferralcentreineasternindia AT raghunathchatterjee associationofhumanleukocyteantigencw6inpsoriasispatientswithdiseaseseverityandmorphologicalpatternsacrosscontrolstudyinatertiarycarereferralcentreineasternindia |