Evaluation of BAX and BCL-2 Gene Expression Levels and Apoptosis in Resveratrol Affected Human Leukemic Cell Line: CCRF-CEM
Objectives: Acute lymphoblastic leukemia (ALL) is considered one of the common types of cancers in childhood with an incidence of up to 25%. In addition, drug resistance is a phenomenon which reduces the chances of overcoming cancer. Further, a phytoalexin combination called resveratrol can sensitiz...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Aras Part Medical International Press
2019-04-01
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| Series: | Crescent Journal of Medical and Biological Sciences |
| Subjects: | |
| Online Access: | http://www.cjmb.org/pdf.php?id=228 |
| _version_ | 1818048980836155392 |
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| author | Shiva Pour Vahdani Taghi Khanzadeh Fattaneh Karimi Aylin Jahanban Esfahlan Somayeh Ghavipanjeh Amin Ghasemi Milad Zadi Heydarabad Hakim Azizi Akbar Darbin Arash Khorrami Ako Azimi |
| author_facet | Shiva Pour Vahdani Taghi Khanzadeh Fattaneh Karimi Aylin Jahanban Esfahlan Somayeh Ghavipanjeh Amin Ghasemi Milad Zadi Heydarabad Hakim Azizi Akbar Darbin Arash Khorrami Ako Azimi |
| author_sort | Shiva Pour Vahdani |
| collection | DOAJ |
| description | Objectives: Acute lymphoblastic leukemia (ALL) is considered one of the common types of cancers in childhood with an incidence of up to 25%. In addition, drug resistance is a phenomenon which reduces the chances of overcoming cancer. Further, a phytoalexin combination called resveratrol can sensitize the leukemic cells to apoptotic cell death. Due to the importance of the above-mentioned issues, the present study aimed to evaluate the effect of resveratrol on BAX and BCL-2 expression levels and apoptosis induction. Materials and Methods: CCRF-CEM cultured cells were treated by resveratrol at doses of 15, 50, and 100 µM based on previous studies. Furthermore, RT Polymerase chain reaction (PCR) was conducted to assess the BAX and BCL-2 gene expression. Moreover, the amount of apoptosis induction was analyzed by annexin V staining method. Results: Based on the results, time and concentration were found to play a critical role in resveratrol-induced apoptosis. Additionally, BAX upregulation and BCL-2 downregulation excreted by resveratrol in CCRF-CEM cells resulted in predisposing these cells to apoptosis. Conclusions: In general, it was revealed that resveratrol could have a chemo-preventive activity by modifying the expression of BAX and BCL-2 genes. Finally, resveratrol was found to be a supplement drug in anti-leukemic therapy. |
| first_indexed | 2024-12-10T10:30:18Z |
| format | Article |
| id | doaj.art-d41f8617c3a341e886553f22821f420c |
| institution | Directory Open Access Journal |
| issn | 2148-9696 |
| language | English |
| last_indexed | 2024-12-10T10:30:18Z |
| publishDate | 2019-04-01 |
| publisher | Aras Part Medical International Press |
| record_format | Article |
| series | Crescent Journal of Medical and Biological Sciences |
| spelling | doaj.art-d41f8617c3a341e886553f22821f420c2022-12-22T01:52:35ZengAras Part Medical International PressCrescent Journal of Medical and Biological Sciences2148-96962019-04-0162209213cjmb-5071Evaluation of BAX and BCL-2 Gene Expression Levels and Apoptosis in Resveratrol Affected Human Leukemic Cell Line: CCRF-CEMShiva Pour Vahdani0Taghi Khanzadeh1Fattaneh Karimi2Aylin Jahanban Esfahlan3Somayeh Ghavipanjeh4Amin Ghasemi5Milad Zadi Heydarabad6Hakim Azizi7Akbar Darbin8Arash Khorrami9Ako Azimi10Student Research Committee, Maragheh University of Medical Sciences, Maragheh, Iran.Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.Department of Community and Mental Health Nursing, School of Nursing and Midwifery, Maragheh University of Medical Sciences, Maragheh, Iran.Department of Nursing, School of Nursing and Midwifery, Maragheh University of Medical Sciences, Maragheh, Iran.Department of Psychiatric Nursing, Maragheh University of Medical Sciences, Maragheh, Iran.Student Research Committee, Maragheh University of Medical Sciences, Maragheh, Iran.Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.Department of Medical Parasitology, Zabol University of Medical Sciences, Zabol, Iran.Shahid Madani Hospital, Tabriz University of Medical Sciences, Tabriz, Iran.Department of Basic Sciences, Maragheh University of Medical Sciences, Maragheh, Iran.Department of Basic Sciences, Maragheh University of Medical Sciences, Maragheh, Iran.Objectives: Acute lymphoblastic leukemia (ALL) is considered one of the common types of cancers in childhood with an incidence of up to 25%. In addition, drug resistance is a phenomenon which reduces the chances of overcoming cancer. Further, a phytoalexin combination called resveratrol can sensitize the leukemic cells to apoptotic cell death. Due to the importance of the above-mentioned issues, the present study aimed to evaluate the effect of resveratrol on BAX and BCL-2 expression levels and apoptosis induction. Materials and Methods: CCRF-CEM cultured cells were treated by resveratrol at doses of 15, 50, and 100 µM based on previous studies. Furthermore, RT Polymerase chain reaction (PCR) was conducted to assess the BAX and BCL-2 gene expression. Moreover, the amount of apoptosis induction was analyzed by annexin V staining method. Results: Based on the results, time and concentration were found to play a critical role in resveratrol-induced apoptosis. Additionally, BAX upregulation and BCL-2 downregulation excreted by resveratrol in CCRF-CEM cells resulted in predisposing these cells to apoptosis. Conclusions: In general, it was revealed that resveratrol could have a chemo-preventive activity by modifying the expression of BAX and BCL-2 genes. Finally, resveratrol was found to be a supplement drug in anti-leukemic therapy.http://www.cjmb.org/pdf.php?id=228leukemiaresveratrolbcl-2 associated xbcl-2 geneapoptosis |
| spellingShingle | Shiva Pour Vahdani Taghi Khanzadeh Fattaneh Karimi Aylin Jahanban Esfahlan Somayeh Ghavipanjeh Amin Ghasemi Milad Zadi Heydarabad Hakim Azizi Akbar Darbin Arash Khorrami Ako Azimi Evaluation of BAX and BCL-2 Gene Expression Levels and Apoptosis in Resveratrol Affected Human Leukemic Cell Line: CCRF-CEM Crescent Journal of Medical and Biological Sciences leukemia resveratrol bcl-2 associated x bcl-2 gene apoptosis |
| title | Evaluation of BAX and BCL-2 Gene Expression Levels and Apoptosis in Resveratrol Affected Human Leukemic Cell Line: CCRF-CEM |
| title_full | Evaluation of BAX and BCL-2 Gene Expression Levels and Apoptosis in Resveratrol Affected Human Leukemic Cell Line: CCRF-CEM |
| title_fullStr | Evaluation of BAX and BCL-2 Gene Expression Levels and Apoptosis in Resveratrol Affected Human Leukemic Cell Line: CCRF-CEM |
| title_full_unstemmed | Evaluation of BAX and BCL-2 Gene Expression Levels and Apoptosis in Resveratrol Affected Human Leukemic Cell Line: CCRF-CEM |
| title_short | Evaluation of BAX and BCL-2 Gene Expression Levels and Apoptosis in Resveratrol Affected Human Leukemic Cell Line: CCRF-CEM |
| title_sort | evaluation of bax and bcl 2 gene expression levels and apoptosis in resveratrol affected human leukemic cell line ccrf cem |
| topic | leukemia resveratrol bcl-2 associated x bcl-2 gene apoptosis |
| url | http://www.cjmb.org/pdf.php?id=228 |
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