Lipid Composition of Sheffersomyces stipitis M12 Strain Grown on Glycerol as a Carbon Source
Research background. In this study the content and composition of lipids in ergosterol-reduced Sheffersomyces stipitis M12 strain grown on glycerol as a carbon source is determined. Blocking the ergosterol synthesis route in yeast cells is a recently proposed method for increasing S-adenosyl-l-methi...
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| Format: | Article |
| Language: | English |
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University of Zagreb Faculty of Food Technology and Biotechnology
2020-01-01
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| Series: | Food Technology and Biotechnology |
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| Online Access: | https://hrcak.srce.hr/file/351854 |
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| author | Stela Križanović Damir Stanzer Jasna Mrvčić Karla Hanousek-Čiča Elizabeta Kralj Gordana Čanadi-Jurešić |
| author_facet | Stela Križanović Damir Stanzer Jasna Mrvčić Karla Hanousek-Čiča Elizabeta Kralj Gordana Čanadi-Jurešić |
| author_sort | Stela Križanović |
| collection | DOAJ |
| description | Research background. In this study the content and composition of lipids in ergosterol-reduced Sheffersomyces stipitis M12 strain grown on glycerol as a carbon source is determined. Blocking the ergosterol synthesis route in yeast cells is a recently proposed method for increasing S-adenosyl-l-methionine (SAM) production.
Experimental approach. The batch cultivation of M12 yeast was carried out under aerobic conditions in a laboratory bioreactor with glycerol as carbon source, and with pulsed addition of methionine. Glycerol and SAM content were monitored by high-performance liquid chromatography, while fatty acid composition of different lipid classes, separated by solid phase extraction, was determined by gas chromatography.
Results and conclusion. Despite the reduced amount of ergosterol in yeast cells, thanks to the reorganized lipid metabolism, M12 strain achieved high biomass yield and SAM production. Neutral lipids prevailed (making more than 75 % of total lipids), but their content and composition differed significantly in the two tested types of yeast. Unsaturated and C18 fatty acids prevailed in both the M12 strain and wild type. In all fractions except free fatty acids, the index of unsaturation in M12 strain was lower than in the wild strain. Our tested strain adjusts itself by changing the content of lipids (mainly phospholipids, sterols and sterol esters), and with desaturation adjustments, to maintain proper functioning and fulfil increased energy needs.
Novelty and scientific contribution. Reorganization of S. stipitis lipid composition caused by blocking the metabolic pathway of ergosterol synthesis was presented. A simple scheme of actual lipid metabolism during active SAM production in S. stipitis, grown on glycerol was constructed and shown. This fundamental knowledge of lipid metabolic pathways will be a helpful tool in improving S. stipitis as an expression host and a model organism, opening new perspectives for its applied research. |
| first_indexed | 2024-04-24T09:19:44Z |
| format | Article |
| id | doaj.art-d4254ad9a76947a3bd15766bebf0a296 |
| institution | Directory Open Access Journal |
| issn | 1330-9862 1334-2606 |
| language | English |
| last_indexed | 2024-04-24T09:19:44Z |
| publishDate | 2020-01-01 |
| publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
| record_format | Article |
| series | Food Technology and Biotechnology |
| spelling | doaj.art-d4254ad9a76947a3bd15766bebf0a2962024-04-15T16:21:24ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062020-01-0158220321310.17113/ftb.58.02.20.6540Lipid Composition of Sheffersomyces stipitis M12 Strain Grown on Glycerol as a Carbon SourceStela Križanović0Damir Stanzer1Jasna Mrvčić2Karla Hanousek-Čiča3Elizabeta Kralj4Gordana Čanadi-Jurešić5Division for Marine and Environmental Research, Ruđer Bošković Institute, Bijenička 54, 10000 Zagreb, CroatiaDepartment of Food Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, CroatiaDepartment of Food Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, CroatiaDepartment of Food Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, CroatiaKarlovac University of Applied Science, Trg Josipa Jurja Strossmayera 9, 47000 Karlovac, CroatiaDepartment of Medical Chemistry, Biochemistry and Clinical Chemistry, Faculty of Medicine, University of Rijeka, B. Branchetta 20, 51000 Rijeka, CroatiaResearch background. In this study the content and composition of lipids in ergosterol-reduced Sheffersomyces stipitis M12 strain grown on glycerol as a carbon source is determined. Blocking the ergosterol synthesis route in yeast cells is a recently proposed method for increasing S-adenosyl-l-methionine (SAM) production. Experimental approach. The batch cultivation of M12 yeast was carried out under aerobic conditions in a laboratory bioreactor with glycerol as carbon source, and with pulsed addition of methionine. Glycerol and SAM content were monitored by high-performance liquid chromatography, while fatty acid composition of different lipid classes, separated by solid phase extraction, was determined by gas chromatography. Results and conclusion. Despite the reduced amount of ergosterol in yeast cells, thanks to the reorganized lipid metabolism, M12 strain achieved high biomass yield and SAM production. Neutral lipids prevailed (making more than 75 % of total lipids), but their content and composition differed significantly in the two tested types of yeast. Unsaturated and C18 fatty acids prevailed in both the M12 strain and wild type. In all fractions except free fatty acids, the index of unsaturation in M12 strain was lower than in the wild strain. Our tested strain adjusts itself by changing the content of lipids (mainly phospholipids, sterols and sterol esters), and with desaturation adjustments, to maintain proper functioning and fulfil increased energy needs. Novelty and scientific contribution. Reorganization of S. stipitis lipid composition caused by blocking the metabolic pathway of ergosterol synthesis was presented. A simple scheme of actual lipid metabolism during active SAM production in S. stipitis, grown on glycerol was constructed and shown. This fundamental knowledge of lipid metabolic pathways will be a helpful tool in improving S. stipitis as an expression host and a model organism, opening new perspectives for its applied research.https://hrcak.srce.hr/file/351854lipid compositionSheffersomyces stipitisS-adenosyl-L-methionine productionlipid metabolism scheme |
| spellingShingle | Stela Križanović Damir Stanzer Jasna Mrvčić Karla Hanousek-Čiča Elizabeta Kralj Gordana Čanadi-Jurešić Lipid Composition of Sheffersomyces stipitis M12 Strain Grown on Glycerol as a Carbon Source Food Technology and Biotechnology lipid composition Sheffersomyces stipitis S-adenosyl-L-methionine production lipid metabolism scheme |
| title | Lipid Composition of Sheffersomyces stipitis M12 Strain Grown on Glycerol as a Carbon Source |
| title_full | Lipid Composition of Sheffersomyces stipitis M12 Strain Grown on Glycerol as a Carbon Source |
| title_fullStr | Lipid Composition of Sheffersomyces stipitis M12 Strain Grown on Glycerol as a Carbon Source |
| title_full_unstemmed | Lipid Composition of Sheffersomyces stipitis M12 Strain Grown on Glycerol as a Carbon Source |
| title_short | Lipid Composition of Sheffersomyces stipitis M12 Strain Grown on Glycerol as a Carbon Source |
| title_sort | lipid composition of sheffersomyces stipitis m12 strain grown on glycerol as a carbon source |
| topic | lipid composition Sheffersomyces stipitis S-adenosyl-L-methionine production lipid metabolism scheme |
| url | https://hrcak.srce.hr/file/351854 |
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