Whole blood as an alternative to peripheral blood mononuclear cell for detection of total HIV-1 DNA
Abstract Background A more time saving, convenient, reproducible, and scalable method is needed to assess total HIV-1 DNA levels. Methods Frozen whole blood and peripheral blood mononuclear cell (PBMC) samples both 200 μl at the same point were used to detect total HIV-1 DNA. Automatic extraction of...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-12-01
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| Series: | BMC Infectious Diseases |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12879-020-05675-3 |
| _version_ | 1818329716622360576 |
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| author | Ling Lin Yong-Song Yue Ni-Dan Wang Lei-Yan Wei Yang Han Xiao-Jing Song Zhi-Feng Qiu Wei Cao Tai-Sheng Li |
| author_facet | Ling Lin Yong-Song Yue Ni-Dan Wang Lei-Yan Wei Yang Han Xiao-Jing Song Zhi-Feng Qiu Wei Cao Tai-Sheng Li |
| author_sort | Ling Lin |
| collection | DOAJ |
| description | Abstract Background A more time saving, convenient, reproducible, and scalable method is needed to assess total HIV-1 DNA levels. Methods Frozen whole blood and peripheral blood mononuclear cell (PBMC) samples both 200 μl at the same point were used to detect total HIV-1 DNA. Automatic extraction of total HIV-1 DNA was used to ensure the consistency of sample extraction efficiency. The detection reagent was HIV-1 DNA quantitative detection kit and real-time quantitative PCR was utilized. Results Of the 44 included patients, 42 were male and 2 were female, with a median age of 33 years. Thirty-three cases were collected after receiving antiviral treatment, with a median duration of treatment of 3 months, and the other 11 cases were collected before antiviral treatment. The median viral load was 1.83 log10 copies/mL, the median CD4 and CD8 count were 94 and 680 cells/μL, and the median CD4/CD8 ratio was 0.18. The results of the two samples were 3.02 ± 0.39 log10 copies/106 PBMCs in PBMC samples and 3.05 ± 0.40 log10 copies/106 PBMCs in whole blood samples. The detection results of the two methods were highly correlated and consistent by using paired t test (P = 0.370), pearson correlation (r = 0.887, P < 0.0001) and intra-group correlation coefficient (ICC = 0.887, P < 0.0001) and bland-altman [4.55% points were outside the 95% limits of agreement (− 0.340 ~ 0.390)]. Conclusions The results of the whole blood sample test for total HIV-1 DNA are consistent with those of PBMC samples. In a clinical setting it is recommended to use whole blood samples directly for the evaluation of the HIV reservoir. |
| first_indexed | 2024-12-13T12:52:29Z |
| format | Article |
| id | doaj.art-d428099e57f34431bde6f2ddbcd37b93 |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-12-13T12:52:29Z |
| publishDate | 2020-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-d428099e57f34431bde6f2ddbcd37b932022-12-21T23:45:18ZengBMCBMC Infectious Diseases1471-23342020-12-012011610.1186/s12879-020-05675-3Whole blood as an alternative to peripheral blood mononuclear cell for detection of total HIV-1 DNALing Lin0Yong-Song Yue1Ni-Dan Wang2Lei-Yan Wei3Yang Han4Xiao-Jing Song5Zhi-Feng Qiu6Wei Cao7Tai-Sheng Li8Department of Infectious Diseases, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical SciencesDepartment of Infectious Diseases, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical SciencesDepartment of Infectious Diseases, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical SciencesDepartment of Infectious Diseases, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical SciencesDepartment of Infectious Diseases, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical SciencesDepartment of Infectious Diseases, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical SciencesDepartment of Infectious Diseases, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical SciencesDepartment of Infectious Diseases, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical SciencesDepartment of Infectious Diseases, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical SciencesAbstract Background A more time saving, convenient, reproducible, and scalable method is needed to assess total HIV-1 DNA levels. Methods Frozen whole blood and peripheral blood mononuclear cell (PBMC) samples both 200 μl at the same point were used to detect total HIV-1 DNA. Automatic extraction of total HIV-1 DNA was used to ensure the consistency of sample extraction efficiency. The detection reagent was HIV-1 DNA quantitative detection kit and real-time quantitative PCR was utilized. Results Of the 44 included patients, 42 were male and 2 were female, with a median age of 33 years. Thirty-three cases were collected after receiving antiviral treatment, with a median duration of treatment of 3 months, and the other 11 cases were collected before antiviral treatment. The median viral load was 1.83 log10 copies/mL, the median CD4 and CD8 count were 94 and 680 cells/μL, and the median CD4/CD8 ratio was 0.18. The results of the two samples were 3.02 ± 0.39 log10 copies/106 PBMCs in PBMC samples and 3.05 ± 0.40 log10 copies/106 PBMCs in whole blood samples. The detection results of the two methods were highly correlated and consistent by using paired t test (P = 0.370), pearson correlation (r = 0.887, P < 0.0001) and intra-group correlation coefficient (ICC = 0.887, P < 0.0001) and bland-altman [4.55% points were outside the 95% limits of agreement (− 0.340 ~ 0.390)]. Conclusions The results of the whole blood sample test for total HIV-1 DNA are consistent with those of PBMC samples. In a clinical setting it is recommended to use whole blood samples directly for the evaluation of the HIV reservoir.https://doi.org/10.1186/s12879-020-05675-3AIDSHIV-1 DNAPeripheral blood mononuclear cellReservoirWhole blood |
| spellingShingle | Ling Lin Yong-Song Yue Ni-Dan Wang Lei-Yan Wei Yang Han Xiao-Jing Song Zhi-Feng Qiu Wei Cao Tai-Sheng Li Whole blood as an alternative to peripheral blood mononuclear cell for detection of total HIV-1 DNA BMC Infectious Diseases AIDS HIV-1 DNA Peripheral blood mononuclear cell Reservoir Whole blood |
| title | Whole blood as an alternative to peripheral blood mononuclear cell for detection of total HIV-1 DNA |
| title_full | Whole blood as an alternative to peripheral blood mononuclear cell for detection of total HIV-1 DNA |
| title_fullStr | Whole blood as an alternative to peripheral blood mononuclear cell for detection of total HIV-1 DNA |
| title_full_unstemmed | Whole blood as an alternative to peripheral blood mononuclear cell for detection of total HIV-1 DNA |
| title_short | Whole blood as an alternative to peripheral blood mononuclear cell for detection of total HIV-1 DNA |
| title_sort | whole blood as an alternative to peripheral blood mononuclear cell for detection of total hiv 1 dna |
| topic | AIDS HIV-1 DNA Peripheral blood mononuclear cell Reservoir Whole blood |
| url | https://doi.org/10.1186/s12879-020-05675-3 |
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