Osteogenic actions of the osteogenic growth peptide on bovine marrow mesenchymal stromal cells in culture
The osteogenic growth peptide (OGP) regulates the differentiation of marrow mesenchymal stem cells derived from human and rodent cell lines into osteoblasts. Whether OGP directly regulates the bovine marrow mesenchymal stem cells differentiating into osteoblasts remains unknown. In this study, we ev...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Czech Academy of Agricultural Sciences
2008-09-01
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| Series: | Veterinární Medicína |
| Subjects: | |
| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-200809-0003_osteogenic-actions-of-the-osteogenic-growth-peptide-on-bovine-marrow-mesenchymal-stromal-cells-in-culture.php |
| _version_ | 1797897689195610112 |
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| author | H.B. Zhu D.Z. Guo S.J. Yang Y.H. Zhang H. Wang H.T. Guo Y. Zhang D.C. Cheng |
| author_facet | H.B. Zhu D.Z. Guo S.J. Yang Y.H. Zhang H. Wang H.T. Guo Y. Zhang D.C. Cheng |
| author_sort | H.B. Zhu |
| collection | DOAJ |
| description | The osteogenic growth peptide (OGP) regulates the differentiation of marrow mesenchymal stem cells derived from human and rodent cell lines into osteoblasts. Whether OGP directly regulates the bovine marrow mesenchymal stem cells differentiating into osteoblasts remains unknown. In this study, we evaluated the effects of OGP on the growth and differentiation of bovine marrow mesenchymal stem cells in culture. Our results showed that OGP promoted osteogenic differentiation of the bovine stem cells. OGP increased alkaline phosphatase (ALP) activity and mineralized nodule formation, and stimulated osteoblast-specific mRNA expression of Osteocalcin (BGP). On the other hand, OGP dose-dependently stimulated the expression of endothelial nitric oxide synthases. These results show for the first time a direct osteogenic effect of OGP on bovine marrow stromal cells in culture, which could be mediated by induction of endothelial nitric oxide synthases. |
| first_indexed | 2024-04-10T08:02:19Z |
| format | Article |
| id | doaj.art-d428d64bf45140519e485d001bc6fbff |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-04-10T08:02:19Z |
| publishDate | 2008-09-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj.art-d428d64bf45140519e485d001bc6fbff2023-02-23T03:49:34ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922008-09-0153950150910.17221/1981-VETMEDvet-200809-0003Osteogenic actions of the osteogenic growth peptide on bovine marrow mesenchymal stromal cells in cultureH.B. Zhu0D.Z. Guo1S.J. Yang2Y.H. Zhang3H. Wang4H.T. Guo5Y. Zhang6D.C. Cheng7Huazhong Agricultural University, College of Veterinary Medicine, Wuhan, ChinaHuazhong Agricultural University, College of Veterinary Medicine, Wuhan, ChinaHuazhong Agricultural University, College of Veterinary Medicine, Wuhan, ChinaHuazhong Agricultural University, College of Veterinary Medicine, Wuhan, ChinaHuazhong Agricultural University, College of Veterinary Medicine, Wuhan, ChinaHuazhong Agricultural University, College of Veterinary Medicine, Wuhan, ChinaHuazhong Agricultural University, College of Veterinary Medicine, Wuhan, ChinaHuazhong Agricultural University, College of Veterinary Medicine, Wuhan, ChinaThe osteogenic growth peptide (OGP) regulates the differentiation of marrow mesenchymal stem cells derived from human and rodent cell lines into osteoblasts. Whether OGP directly regulates the bovine marrow mesenchymal stem cells differentiating into osteoblasts remains unknown. In this study, we evaluated the effects of OGP on the growth and differentiation of bovine marrow mesenchymal stem cells in culture. Our results showed that OGP promoted osteogenic differentiation of the bovine stem cells. OGP increased alkaline phosphatase (ALP) activity and mineralized nodule formation, and stimulated osteoblast-specific mRNA expression of Osteocalcin (BGP). On the other hand, OGP dose-dependently stimulated the expression of endothelial nitric oxide synthases. These results show for the first time a direct osteogenic effect of OGP on bovine marrow stromal cells in culture, which could be mediated by induction of endothelial nitric oxide synthases.https://vetmed.agriculturejournals.cz/artkey/vet-200809-0003_osteogenic-actions-of-the-osteogenic-growth-peptide-on-bovine-marrow-mesenchymal-stromal-cells-in-culture.phposteogenic growth peptidebovinemarrow stromal cellsenos |
| spellingShingle | H.B. Zhu D.Z. Guo S.J. Yang Y.H. Zhang H. Wang H.T. Guo Y. Zhang D.C. Cheng Osteogenic actions of the osteogenic growth peptide on bovine marrow mesenchymal stromal cells in culture Veterinární Medicína osteogenic growth peptide bovine marrow stromal cells enos |
| title | Osteogenic actions of the osteogenic growth peptide on bovine marrow mesenchymal stromal cells in culture |
| title_full | Osteogenic actions of the osteogenic growth peptide on bovine marrow mesenchymal stromal cells in culture |
| title_fullStr | Osteogenic actions of the osteogenic growth peptide on bovine marrow mesenchymal stromal cells in culture |
| title_full_unstemmed | Osteogenic actions of the osteogenic growth peptide on bovine marrow mesenchymal stromal cells in culture |
| title_short | Osteogenic actions of the osteogenic growth peptide on bovine marrow mesenchymal stromal cells in culture |
| title_sort | osteogenic actions of the osteogenic growth peptide on bovine marrow mesenchymal stromal cells in culture |
| topic | osteogenic growth peptide bovine marrow stromal cells enos |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-200809-0003_osteogenic-actions-of-the-osteogenic-growth-peptide-on-bovine-marrow-mesenchymal-stromal-cells-in-culture.php |
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