In-vitro screening of antibacterial potentials of Aspilia africana leaves.
In-vitro screening of antibacterial potentials of aqueous, ethanolic and methanolic extracts of Aspilia africana leaves was carried out using agar well diffusion method. Data obtained from this study indicated that the leaf extracts of A. africana possessed antibacterial activity against Staphyloco...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Faculty of Biosciences
2013-05-01
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| Series: | The Bioscientist |
| Subjects: | |
| Online Access: | https://bioscientistjournal.com/index.php/The_Bioscientist/article/view/57 |
| Summary: | In-vitro screening of antibacterial potentials of aqueous, ethanolic and methanolic extracts of Aspilia africana leaves was carried out using agar well diffusion method. Data obtained from this study indicated that the leaf extracts of A. africana possessed antibacterial activity against Staphylococcus aureus, Pseudomonas aeruginosa, and Klebsiella pneumoniae. The methanolic and ethanolic extract of A. africana showed antibacterial activity with the diameter of zone of inhibition of 9.0 - 16.0 mm and 7.17 - 14.17 mm against S. aureus, 8.0 – 14.67 mm and 8.83 - 13.17 mm against K. pneumonia and 7.0 - 14.0 mm and 7.50 - 12.33 mm against P. aeruginosa respectively. Methanolic extract of A. africana was observed to be more potent, inhibiting all isolates thus showing higher antibacterial activity than the ethanolic and aqueous extracts. The efficacy of the extracts was further exhibited as the S. aureus used for the study, was resistant to the positive control (gentamicin) while been sensitive to the extracts. The test organisms were not sensitive to the extractants alone. The efficacy of the extracts towards inhibition of the micro organisms increased with increased concentration. The Minimum Inhibitory Concentration and Minimum Bactericidal Concentration of the extracts on the test organisms also increased in the following order; aqueous < ethanolic < methanolic. Findings from this study further showed that plants are potential sources of new drugs for treating infections caused by these antibiotic resistant clinical pathogens
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| ISSN: | 2630-7103 2630-7111 |