Bone Fracture-Treatment Method: Fixing 3D-Printed Polycaprolactone Scaffolds with Hydrogel Type Bone-Derived Extracellular Matrix and β-Tricalcium Phosphate as an Osteogenic Promoter
Bone formation and growth are crucial for treating bone fractures. Improving bone-reconstruction methods using autologous bone and synthetic implants can reduce the recovery time. Here, we investigated three treatments using two different materials, a bone-derived decellularized extracellular matrix...
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MDPI AG
2021-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/16/9084 |
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| author | Seokhwan Yun Dami Choi Dong-Jin Choi Songwan Jin Won-Soo Yun Jung-Bo Huh Jin-Hyung Shim |
| author_facet | Seokhwan Yun Dami Choi Dong-Jin Choi Songwan Jin Won-Soo Yun Jung-Bo Huh Jin-Hyung Shim |
| author_sort | Seokhwan Yun |
| collection | DOAJ |
| description | Bone formation and growth are crucial for treating bone fractures. Improving bone-reconstruction methods using autologous bone and synthetic implants can reduce the recovery time. Here, we investigated three treatments using two different materials, a bone-derived decellularized extracellular matrix (bdECM) and β-tricalcium phosphate (β-TCP), individually and in combination, as osteogenic promoter between bone and 3D-printed polycaprolactone scaffold (6-mm diameter) in rat calvarial defects (8-mm critical diameter). The materials were tested with a human pre-osteoblast cell line (MG63) to determine the effects of the osteogenic promoter on bone formation in vitro. A polycaprolactone (PCL) scaffold with a porous structure was placed at the center of the in vivo rat calvarial defects. The gap between the defective bone and PCL scaffold was filled with each material. Animals were sacrificed four weeks post-implantation, and skull samples were preserved for analysis. The preserved samples were scanned by micro-computed tomography and analyzed histologically to examine the clinical benefits of the materials. The bdECM–β-TCP mixture showed faster bone formation and a lower inflammatory response in the rats. Therefore, our results imply that a bdECM–β-TCP mixture is an ideal osteogenic promoter for treating fractures. |
| first_indexed | 2024-03-10T08:44:19Z |
| format | Article |
| id | doaj.art-d4341aa1d5934fe4b338412c666d5c5a |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T08:44:19Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-d4341aa1d5934fe4b338412c666d5c5a2023-11-22T08:05:13ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-08-012216908410.3390/ijms22169084Bone Fracture-Treatment Method: Fixing 3D-Printed Polycaprolactone Scaffolds with Hydrogel Type Bone-Derived Extracellular Matrix and β-Tricalcium Phosphate as an Osteogenic PromoterSeokhwan Yun0Dami Choi1Dong-Jin Choi2Songwan Jin3Won-Soo Yun4Jung-Bo Huh5Jin-Hyung Shim6Department of Mechanical Engineering, Korea Polytechnic University, Siheung-si 15073, KoreaResearch Institute, T&R Biofab Co., Ltd., Siheung-si 15073, KoreaDepartment of Mechanical Engineering, Korea Polytechnic University, Siheung-si 15073, KoreaDepartment of Mechanical Engineering, Korea Polytechnic University, Siheung-si 15073, KoreaDepartment of Mechanical Engineering, Korea Polytechnic University, Siheung-si 15073, KoreaDepartment of Prosthodontics, Dental Research Institute, Dental and Life Sciences Institute, School of Dentistry, Pusan National University, Yangsan-si 50612, KoreaDepartment of Mechanical Engineering, Korea Polytechnic University, Siheung-si 15073, KoreaBone formation and growth are crucial for treating bone fractures. Improving bone-reconstruction methods using autologous bone and synthetic implants can reduce the recovery time. Here, we investigated three treatments using two different materials, a bone-derived decellularized extracellular matrix (bdECM) and β-tricalcium phosphate (β-TCP), individually and in combination, as osteogenic promoter between bone and 3D-printed polycaprolactone scaffold (6-mm diameter) in rat calvarial defects (8-mm critical diameter). The materials were tested with a human pre-osteoblast cell line (MG63) to determine the effects of the osteogenic promoter on bone formation in vitro. A polycaprolactone (PCL) scaffold with a porous structure was placed at the center of the in vivo rat calvarial defects. The gap between the defective bone and PCL scaffold was filled with each material. Animals were sacrificed four weeks post-implantation, and skull samples were preserved for analysis. The preserved samples were scanned by micro-computed tomography and analyzed histologically to examine the clinical benefits of the materials. The bdECM–β-TCP mixture showed faster bone formation and a lower inflammatory response in the rats. Therefore, our results imply that a bdECM–β-TCP mixture is an ideal osteogenic promoter for treating fractures.https://www.mdpi.com/1422-0067/22/16/9084β-tricalcium phosphatebone-derived extracellular matrixbone fracturepolycaprolactone3D printing |
| spellingShingle | Seokhwan Yun Dami Choi Dong-Jin Choi Songwan Jin Won-Soo Yun Jung-Bo Huh Jin-Hyung Shim Bone Fracture-Treatment Method: Fixing 3D-Printed Polycaprolactone Scaffolds with Hydrogel Type Bone-Derived Extracellular Matrix and β-Tricalcium Phosphate as an Osteogenic Promoter International Journal of Molecular Sciences β-tricalcium phosphate bone-derived extracellular matrix bone fracture polycaprolactone 3D printing |
| title | Bone Fracture-Treatment Method: Fixing 3D-Printed Polycaprolactone Scaffolds with Hydrogel Type Bone-Derived Extracellular Matrix and β-Tricalcium Phosphate as an Osteogenic Promoter |
| title_full | Bone Fracture-Treatment Method: Fixing 3D-Printed Polycaprolactone Scaffolds with Hydrogel Type Bone-Derived Extracellular Matrix and β-Tricalcium Phosphate as an Osteogenic Promoter |
| title_fullStr | Bone Fracture-Treatment Method: Fixing 3D-Printed Polycaprolactone Scaffolds with Hydrogel Type Bone-Derived Extracellular Matrix and β-Tricalcium Phosphate as an Osteogenic Promoter |
| title_full_unstemmed | Bone Fracture-Treatment Method: Fixing 3D-Printed Polycaprolactone Scaffolds with Hydrogel Type Bone-Derived Extracellular Matrix and β-Tricalcium Phosphate as an Osteogenic Promoter |
| title_short | Bone Fracture-Treatment Method: Fixing 3D-Printed Polycaprolactone Scaffolds with Hydrogel Type Bone-Derived Extracellular Matrix and β-Tricalcium Phosphate as an Osteogenic Promoter |
| title_sort | bone fracture treatment method fixing 3d printed polycaprolactone scaffolds with hydrogel type bone derived extracellular matrix and β tricalcium phosphate as an osteogenic promoter |
| topic | β-tricalcium phosphate bone-derived extracellular matrix bone fracture polycaprolactone 3D printing |
| url | https://www.mdpi.com/1422-0067/22/16/9084 |
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