Optical Aggregation of Gold Nanoparticles for SERS Detection of Proteins and Toxins in Liquid Environment: Towards Ultrasensitive and Selective Detection
Optical forces are used to aggregate plasmonic nanoparticles and create SERS–active hot spots in liquid. When biomolecules are added to the nanoparticles, high sensitivity SERS detection can be accomplished. Here, we pursue studies on Bovine Serum Albumin (BSA) detection, investigating the BSA–nanor...
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| Language: | English |
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MDPI AG
2018-03-01
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| Series: | Materials |
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| Online Access: | http://www.mdpi.com/1996-1944/11/3/440 |
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| author | Antonino Foti Cristiano D’Andrea Valentina Villari Norberto Micali Maria Grazia Donato Barbara Fazio Onofrio M. Maragò Raymond Gillibert Marc Lamy de la Chapelle Pietro G. Gucciardi |
| author_facet | Antonino Foti Cristiano D’Andrea Valentina Villari Norberto Micali Maria Grazia Donato Barbara Fazio Onofrio M. Maragò Raymond Gillibert Marc Lamy de la Chapelle Pietro G. Gucciardi |
| author_sort | Antonino Foti |
| collection | DOAJ |
| description | Optical forces are used to aggregate plasmonic nanoparticles and create SERS–active hot spots in liquid. When biomolecules are added to the nanoparticles, high sensitivity SERS detection can be accomplished. Here, we pursue studies on Bovine Serum Albumin (BSA) detection, investigating the BSA–nanorod aggregations in a range from 100 µM to 50 nM by combining light scattering, plasmon resonance and SERS, and correlating the SERS signal with the concentration. Experimental data are fitted with a simple model describing the optical aggregation process. We show that BSA–nanorod complexes can be optically printed on non-functionalized glass surfaces, designing custom patterns stable with time. Furthermore, we demonstrate that this methodology can be used to detect catalase and hemoglobin, two Raman resonant biomolecules, at concentrations of 10 nM and 1 pM, respectively, i.e., well beyond the limit of detection of BSA. Finally, we show that nanorods functionalized with specific aptamers can be used to capture and detect Ochratoxin A, a fungal toxin found in food commodities and wine. This experiment represents the first step towards the addition of molecular specificity to this novel biosensor strategy. |
| first_indexed | 2024-04-12T04:49:25Z |
| format | Article |
| id | doaj.art-d43ae6bd1dbe47fb829e5a906bc408d0 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-04-12T04:49:25Z |
| publishDate | 2018-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-d43ae6bd1dbe47fb829e5a906bc408d02022-12-22T03:47:22ZengMDPI AGMaterials1996-19442018-03-0111344010.3390/ma11030440ma11030440Optical Aggregation of Gold Nanoparticles for SERS Detection of Proteins and Toxins in Liquid Environment: Towards Ultrasensitive and Selective DetectionAntonino Foti0Cristiano D’Andrea1Valentina Villari2Norberto Micali3Maria Grazia Donato4Barbara Fazio5Onofrio M. Maragò6Raymond Gillibert7Marc Lamy de la Chapelle8Pietro G. Gucciardi9CNR-IPCF, Istituto per i Processi Chimico-Fisici, Viale F. Stagno D’Alcontres 37, I-98168 Messina, ItalyCNR-IPCF, Istituto per i Processi Chimico-Fisici, Viale F. Stagno D’Alcontres 37, I-98168 Messina, ItalyCNR-IPCF, Istituto per i Processi Chimico-Fisici, Viale F. Stagno D’Alcontres 37, I-98168 Messina, ItalyCNR-IPCF, Istituto per i Processi Chimico-Fisici, Viale F. Stagno D’Alcontres 37, I-98168 Messina, ItalyCNR-IPCF, Istituto per i Processi Chimico-Fisici, Viale F. Stagno D’Alcontres 37, I-98168 Messina, ItalyCNR-IPCF, Istituto per i Processi Chimico-Fisici, Viale F. Stagno D’Alcontres 37, I-98168 Messina, ItalyCNR-IPCF, Istituto per i Processi Chimico-Fisici, Viale F. Stagno D’Alcontres 37, I-98168 Messina, ItalyLaboratoire CSPBAT, Université de Paris 13, Sorbonne Paris Cité, CNRS, 74 Rue Marcel-Cachin, F-93017 Bobigny, FranceLaboratoire CSPBAT, Université de Paris 13, Sorbonne Paris Cité, CNRS, 74 Rue Marcel-Cachin, F-93017 Bobigny, FranceCNR-IPCF, Istituto per i Processi Chimico-Fisici, Viale F. Stagno D’Alcontres 37, I-98168 Messina, ItalyOptical forces are used to aggregate plasmonic nanoparticles and create SERS–active hot spots in liquid. When biomolecules are added to the nanoparticles, high sensitivity SERS detection can be accomplished. Here, we pursue studies on Bovine Serum Albumin (BSA) detection, investigating the BSA–nanorod aggregations in a range from 100 µM to 50 nM by combining light scattering, plasmon resonance and SERS, and correlating the SERS signal with the concentration. Experimental data are fitted with a simple model describing the optical aggregation process. We show that BSA–nanorod complexes can be optically printed on non-functionalized glass surfaces, designing custom patterns stable with time. Furthermore, we demonstrate that this methodology can be used to detect catalase and hemoglobin, two Raman resonant biomolecules, at concentrations of 10 nM and 1 pM, respectively, i.e., well beyond the limit of detection of BSA. Finally, we show that nanorods functionalized with specific aptamers can be used to capture and detect Ochratoxin A, a fungal toxin found in food commodities and wine. This experiment represents the first step towards the addition of molecular specificity to this novel biosensor strategy.http://www.mdpi.com/1996-1944/11/3/440SERSbiosensorgold nanoparticlesaptamerstoxinshemeproteinoptical forcesoptical tweezersoptical patterningcolloids |
| spellingShingle | Antonino Foti Cristiano D’Andrea Valentina Villari Norberto Micali Maria Grazia Donato Barbara Fazio Onofrio M. Maragò Raymond Gillibert Marc Lamy de la Chapelle Pietro G. Gucciardi Optical Aggregation of Gold Nanoparticles for SERS Detection of Proteins and Toxins in Liquid Environment: Towards Ultrasensitive and Selective Detection Materials SERS biosensor gold nanoparticles aptamers toxins hemeprotein optical forces optical tweezers optical patterning colloids |
| title | Optical Aggregation of Gold Nanoparticles for SERS Detection of Proteins and Toxins in Liquid Environment: Towards Ultrasensitive and Selective Detection |
| title_full | Optical Aggregation of Gold Nanoparticles for SERS Detection of Proteins and Toxins in Liquid Environment: Towards Ultrasensitive and Selective Detection |
| title_fullStr | Optical Aggregation of Gold Nanoparticles for SERS Detection of Proteins and Toxins in Liquid Environment: Towards Ultrasensitive and Selective Detection |
| title_full_unstemmed | Optical Aggregation of Gold Nanoparticles for SERS Detection of Proteins and Toxins in Liquid Environment: Towards Ultrasensitive and Selective Detection |
| title_short | Optical Aggregation of Gold Nanoparticles for SERS Detection of Proteins and Toxins in Liquid Environment: Towards Ultrasensitive and Selective Detection |
| title_sort | optical aggregation of gold nanoparticles for sers detection of proteins and toxins in liquid environment towards ultrasensitive and selective detection |
| topic | SERS biosensor gold nanoparticles aptamers toxins hemeprotein optical forces optical tweezers optical patterning colloids |
| url | http://www.mdpi.com/1996-1944/11/3/440 |
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