DETECTION OF HEPATITIS B VIRUS USING ELISA AND REAL TIME PCR TECHNIQUES IN PATIENTS' BLOOD AND SALIVA IN AL- ANBAR PROVINCE
:The present study has been carried out at the laboratories of the department of biology at the college of Education-University of Tikrit for the period from 1st Oct to 1st May 2012. The study consisted of the investigation about Hepatitis B in the saliva as compared with serum by using the ELISA an...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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University of Anbar
2014-11-01
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| Series: | مجلة جامعة الانبار للعلوم الصرفة |
| Subjects: | |
| Online Access: | https://juaps.uoanbar.edu.iq/article_94738_9d232a9c5221362f71ccdc05ea61f84c.pdf |
| _version_ | 1827396266588372992 |
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| author | AMIN S. BADAWY THAER A. HASAN |
| author_facet | AMIN S. BADAWY THAER A. HASAN |
| author_sort | AMIN S. BADAWY |
| collection | DOAJ |
| description | :The present study has been carried out at the laboratories of the department of biology at the college of Education-University of Tikrit for the period from 1st Oct to 1st May 2012. The study consisted of the investigation about Hepatitis B in the saliva as compared with serum by using the ELISA and Real Time PCR techniques. Patients fallen asleep and revisers of the Ramadi General Hospital and the Fallujah General Hospital and who had confirmed cases hepatitis B for the purpose of pattern prove of saliva in the portability infection, where the results of the study were as follows: The results obtained showed that HBsAg antigen was found with 99.4% and (1.101± 0.335) mean ±SD in serum as compared with 48.6% in saliva with a (0.330±0.278) mean ±SD, that gave a noticeable increase when comparing with the control group ( 0.039 ± 0.024 ) and ( 0.017±0.014 ) for serum and saliva respectively. The value of T test for serum and control group equals( 16.25 ), while the value of T test for saliva and control group equals (4.18) which has high significance equals (0.000) with a test value equals ( P < 0.05). The proportion of correlation between saliva and serum in ELISA test equals (0.361) with high significance of test while the value of T test for them equals (28.902) with high significance of test with a test value equals ( P < 0.05). The genetic factor has been used in order to determine the levels of HBV DNA in the samples of positive saliva to test ELISA by using Real Time PCR . 95% from tested serum samples were positive to the viral load. While in saliva 50% from the samples HBV DNA was positive . |
| first_indexed | 2024-03-08T18:48:26Z |
| format | Article |
| id | doaj.art-d43bbcc7abf74ccabc165e782ce89db9 |
| institution | Directory Open Access Journal |
| issn | 1991-8941 2706-6703 |
| language | English |
| last_indexed | 2024-03-08T18:48:26Z |
| publishDate | 2014-11-01 |
| publisher | University of Anbar |
| record_format | Article |
| series | مجلة جامعة الانبار للعلوم الصرفة |
| spelling | doaj.art-d43bbcc7abf74ccabc165e782ce89db92023-12-28T21:57:06ZengUniversity of Anbarمجلة جامعة الانبار للعلوم الصرفة1991-89412706-67032014-11-0173555910.37652/juaps.2013.9473894738DETECTION OF HEPATITIS B VIRUS USING ELISA AND REAL TIME PCR TECHNIQUES IN PATIENTS' BLOOD AND SALIVA IN AL- ANBAR PROVINCEAMIN S. BADAWY0THAER A. HASAN1Tikrit University - College of AgricultureTikrit University - College of Agriculture:The present study has been carried out at the laboratories of the department of biology at the college of Education-University of Tikrit for the period from 1st Oct to 1st May 2012. The study consisted of the investigation about Hepatitis B in the saliva as compared with serum by using the ELISA and Real Time PCR techniques. Patients fallen asleep and revisers of the Ramadi General Hospital and the Fallujah General Hospital and who had confirmed cases hepatitis B for the purpose of pattern prove of saliva in the portability infection, where the results of the study were as follows: The results obtained showed that HBsAg antigen was found with 99.4% and (1.101± 0.335) mean ±SD in serum as compared with 48.6% in saliva with a (0.330±0.278) mean ±SD, that gave a noticeable increase when comparing with the control group ( 0.039 ± 0.024 ) and ( 0.017±0.014 ) for serum and saliva respectively. The value of T test for serum and control group equals( 16.25 ), while the value of T test for saliva and control group equals (4.18) which has high significance equals (0.000) with a test value equals ( P < 0.05). The proportion of correlation between saliva and serum in ELISA test equals (0.361) with high significance of test while the value of T test for them equals (28.902) with high significance of test with a test value equals ( P < 0.05). The genetic factor has been used in order to determine the levels of HBV DNA in the samples of positive saliva to test ELISA by using Real Time PCR . 95% from tested serum samples were positive to the viral load. While in saliva 50% from the samples HBV DNA was positive .https://juaps.uoanbar.edu.iq/article_94738_9d232a9c5221362f71ccdc05ea61f84c.pdfhepatitis belisareal time pcr |
| spellingShingle | AMIN S. BADAWY THAER A. HASAN DETECTION OF HEPATITIS B VIRUS USING ELISA AND REAL TIME PCR TECHNIQUES IN PATIENTS' BLOOD AND SALIVA IN AL- ANBAR PROVINCE مجلة جامعة الانبار للعلوم الصرفة hepatitis b elisa real time pcr |
| title | DETECTION OF HEPATITIS B VIRUS USING ELISA AND REAL TIME PCR TECHNIQUES IN PATIENTS' BLOOD AND SALIVA IN AL- ANBAR PROVINCE |
| title_full | DETECTION OF HEPATITIS B VIRUS USING ELISA AND REAL TIME PCR TECHNIQUES IN PATIENTS' BLOOD AND SALIVA IN AL- ANBAR PROVINCE |
| title_fullStr | DETECTION OF HEPATITIS B VIRUS USING ELISA AND REAL TIME PCR TECHNIQUES IN PATIENTS' BLOOD AND SALIVA IN AL- ANBAR PROVINCE |
| title_full_unstemmed | DETECTION OF HEPATITIS B VIRUS USING ELISA AND REAL TIME PCR TECHNIQUES IN PATIENTS' BLOOD AND SALIVA IN AL- ANBAR PROVINCE |
| title_short | DETECTION OF HEPATITIS B VIRUS USING ELISA AND REAL TIME PCR TECHNIQUES IN PATIENTS' BLOOD AND SALIVA IN AL- ANBAR PROVINCE |
| title_sort | detection of hepatitis b virus using elisa and real time pcr techniques in patients blood and saliva in al anbar province |
| topic | hepatitis b elisa real time pcr |
| url | https://juaps.uoanbar.edu.iq/article_94738_9d232a9c5221362f71ccdc05ea61f84c.pdf |
| work_keys_str_mv | AT aminsbadawy detectionofhepatitisbvirususingelisaandrealtimepcrtechniquesinpatientsbloodandsalivainalanbarprovince AT thaerahasan detectionofhepatitisbvirususingelisaandrealtimepcrtechniquesinpatientsbloodandsalivainalanbarprovince |