Innovative finding of 266-nm laser regulating CD90 levels in SDSCs
Abstract We used light to irradiate skin-derived stem cells and tried to find any cellular protein alterations 24 h after illumination. A 266-nm laser with four intensities was used, and of the nine cell markers that were surveyed in our trials, only CD90 was downregulated at an intensity of 20 μJ f...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-93508-x |
| _version_ | 1818401365332852736 |
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| author | Ray-Ling Hsiao Yen-Chung Chen Mei-Yue Huang Chiang-Yun Chen Yu-Wei Lin Chung-Yi Wu |
| author_facet | Ray-Ling Hsiao Yen-Chung Chen Mei-Yue Huang Chiang-Yun Chen Yu-Wei Lin Chung-Yi Wu |
| author_sort | Ray-Ling Hsiao |
| collection | DOAJ |
| description | Abstract We used light to irradiate skin-derived stem cells and tried to find any cellular protein alterations 24 h after illumination. A 266-nm laser with four intensities was used, and of the nine cell markers that were surveyed in our trials, only CD90 was downregulated at an intensity of 20 μJ for 10 s. Repeated illuminations from the 266-nm laser at seven intensities revealed that CD90 expression was downregulated 14.6–28.8%, depending on light intensity. The maximal effect was noted at an intensity of 30 μJ for 2 s. This innovative finding reveals that a 266-nm laser can regulate protein expression in skin-derivative stem cells. |
| first_indexed | 2024-12-14T07:51:19Z |
| format | Article |
| id | doaj.art-d43eb05cf88d49dd8a6615fdfa84a462 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T07:51:19Z |
| publishDate | 2021-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-d43eb05cf88d49dd8a6615fdfa84a4622022-12-21T23:10:42ZengNature PortfolioScientific Reports2045-23222021-07-011111710.1038/s41598-021-93508-xInnovative finding of 266-nm laser regulating CD90 levels in SDSCsRay-Ling Hsiao0Yen-Chung Chen1Mei-Yue Huang2Chiang-Yun Chen3Yu-Wei Lin4Chung-Yi Wu5Ph.D. Program for Cancer Biology and Drug Discovery, China Medical University and Academia SinicaMaria Von Med-Biotechnology Co., LtdMaria Von Med-Biotechnology Co., LtdGenomics Research Center, Academia SinicaGenomics Research Center, Academia SinicaGenomics Research Center, Academia SinicaAbstract We used light to irradiate skin-derived stem cells and tried to find any cellular protein alterations 24 h after illumination. A 266-nm laser with four intensities was used, and of the nine cell markers that were surveyed in our trials, only CD90 was downregulated at an intensity of 20 μJ for 10 s. Repeated illuminations from the 266-nm laser at seven intensities revealed that CD90 expression was downregulated 14.6–28.8%, depending on light intensity. The maximal effect was noted at an intensity of 30 μJ for 2 s. This innovative finding reveals that a 266-nm laser can regulate protein expression in skin-derivative stem cells.https://doi.org/10.1038/s41598-021-93508-x |
| spellingShingle | Ray-Ling Hsiao Yen-Chung Chen Mei-Yue Huang Chiang-Yun Chen Yu-Wei Lin Chung-Yi Wu Innovative finding of 266-nm laser regulating CD90 levels in SDSCs Scientific Reports |
| title | Innovative finding of 266-nm laser regulating CD90 levels in SDSCs |
| title_full | Innovative finding of 266-nm laser regulating CD90 levels in SDSCs |
| title_fullStr | Innovative finding of 266-nm laser regulating CD90 levels in SDSCs |
| title_full_unstemmed | Innovative finding of 266-nm laser regulating CD90 levels in SDSCs |
| title_short | Innovative finding of 266-nm laser regulating CD90 levels in SDSCs |
| title_sort | innovative finding of 266 nm laser regulating cd90 levels in sdscs |
| url | https://doi.org/10.1038/s41598-021-93508-x |
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