Establishment of Immortalized Human Amniotic Mesenchymal Stem Cells
Human amniotic mesenchymal cells (HAM cells) are known to contain somatic stem cells possessing the characteristics of pluripotency. However, little is known about the biology of these somatic cells because isolated HAM cells from amniotic membrane have a limited lifespan. To overcome this problem,...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2013-02-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.3727/096368912X655055 |
| _version_ | 1818692980967473152 |
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| author | Zan Teng Toshiko Yoshida Motonori Okabe Ayaka Toda Osamu Higuchi Makiko Nogami Noriko Yoneda Kaixuan Zhou Satoru Kyo Touru Kiyono Toshio Nikaido Ph.D. |
| author_facet | Zan Teng Toshiko Yoshida Motonori Okabe Ayaka Toda Osamu Higuchi Makiko Nogami Noriko Yoneda Kaixuan Zhou Satoru Kyo Touru Kiyono Toshio Nikaido Ph.D. |
| author_sort | Zan Teng |
| collection | DOAJ |
| description | Human amniotic mesenchymal cells (HAM cells) are known to contain somatic stem cells possessing the characteristics of pluripotency. However, little is known about the biology of these somatic cells because isolated HAM cells from amniotic membrane have a limited lifespan. To overcome this problem, we attempted to prolong the lifespan of HAM cells by infecting retrovirus encoding human papillomavirus type16E6 and E7 (HPV16E6E7), bmi-1, and/or human telomerase reverse transcriptase (hTERT) genes and investigated their characteristics as stem cells. We confirmed the immortalization of the four lines of cultured HAM cells for about 1 year. Immortalized human amnion mesenchymal cells (iHAM cells) have continued to proliferate over 200 population doublings (PDs). iHAM cells were positive for CD73, CD90, CD105, and CD44 and negative for CD34, CD14, CD45, and HLA-DR. They expressed stem cell markers such as Oct3/4, Sox2, Nanog, Klf4, SSEA4, c-myc, vimentin, and nestin. They showed adipogenic, osteogenic, and chondrogenic differentiation abilities after induction. These results suggested that immortalized cell lines with characteristics of stem cells can be established. iHAM cells with an extended lifespan can be used to produce good experimental models both in vitro and in vivo. |
| first_indexed | 2024-12-17T13:06:25Z |
| format | Article |
| id | doaj.art-d43f454568ae4f599508babf7ae49675 |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-17T13:06:25Z |
| publishDate | 2013-02-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-d43f454568ae4f599508babf7ae496752022-12-21T21:47:14ZengSAGE PublishingCell Transplantation0963-68971555-38922013-02-012210.3727/096368912X655055Establishment of Immortalized Human Amniotic Mesenchymal Stem CellsZan Teng0Toshiko Yoshida1Motonori Okabe2Ayaka Toda3Osamu Higuchi4Makiko Nogami5Noriko Yoneda6Kaixuan Zhou7Satoru Kyo8Touru Kiyono9Toshio Nikaido Ph.D.10Department of Regenerative Medicine, University of Toyama, Graduate School of Medicine and Pharmaceutical Sciences, Toyama, JapanDepartment of Regenerative Medicine, University of Toyama, Graduate School of Medicine and Pharmaceutical Sciences, Toyama, JapanDepartment of Regenerative Medicine, University of Toyama, Graduate School of Medicine and Pharmaceutical Sciences, Toyama, JapanDepartment of Regenerative Medicine, University of Toyama, Graduate School of Medicine and Pharmaceutical Sciences, Toyama, JapanDepartment of Pediatrics, University of Toyama, Graduate School of Medicine and Pharmaceutical Sciences, Toyama, JapanDepartment of Orthopaedic Surgery, University of Toyama, Graduate School of Medicine and Pharmaceutical Sciences, Toyama, JapanDepartment of Obstetrics and Gynecology, University of Toyama, Graduate School of Medicine and Pharmaceutical Sciences, Toyama, JapanDepartment of Regenerative Medicine, University of Toyama, Graduate School of Medicine and Pharmaceutical Sciences, Toyama, JapanDepartment of Obstetrics and Gynecology, School of Medicine, Kanazawa University, Kanazawa, JapanVirology Division, National Cancer Center Research Institute, Tokyo, JapanDepartment of Regenerative Medicine, University of Toyama, Graduate School of Medicine and Pharmaceutical Sciences, Toyama, JapanHuman amniotic mesenchymal cells (HAM cells) are known to contain somatic stem cells possessing the characteristics of pluripotency. However, little is known about the biology of these somatic cells because isolated HAM cells from amniotic membrane have a limited lifespan. To overcome this problem, we attempted to prolong the lifespan of HAM cells by infecting retrovirus encoding human papillomavirus type16E6 and E7 (HPV16E6E7), bmi-1, and/or human telomerase reverse transcriptase (hTERT) genes and investigated their characteristics as stem cells. We confirmed the immortalization of the four lines of cultured HAM cells for about 1 year. Immortalized human amnion mesenchymal cells (iHAM cells) have continued to proliferate over 200 population doublings (PDs). iHAM cells were positive for CD73, CD90, CD105, and CD44 and negative for CD34, CD14, CD45, and HLA-DR. They expressed stem cell markers such as Oct3/4, Sox2, Nanog, Klf4, SSEA4, c-myc, vimentin, and nestin. They showed adipogenic, osteogenic, and chondrogenic differentiation abilities after induction. These results suggested that immortalized cell lines with characteristics of stem cells can be established. iHAM cells with an extended lifespan can be used to produce good experimental models both in vitro and in vivo.https://doi.org/10.3727/096368912X655055 |
| spellingShingle | Zan Teng Toshiko Yoshida Motonori Okabe Ayaka Toda Osamu Higuchi Makiko Nogami Noriko Yoneda Kaixuan Zhou Satoru Kyo Touru Kiyono Toshio Nikaido Ph.D. Establishment of Immortalized Human Amniotic Mesenchymal Stem Cells Cell Transplantation |
| title | Establishment of Immortalized Human Amniotic Mesenchymal Stem Cells |
| title_full | Establishment of Immortalized Human Amniotic Mesenchymal Stem Cells |
| title_fullStr | Establishment of Immortalized Human Amniotic Mesenchymal Stem Cells |
| title_full_unstemmed | Establishment of Immortalized Human Amniotic Mesenchymal Stem Cells |
| title_short | Establishment of Immortalized Human Amniotic Mesenchymal Stem Cells |
| title_sort | establishment of immortalized human amniotic mesenchymal stem cells |
| url | https://doi.org/10.3727/096368912X655055 |
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