CobB-mediated deacetylation of the chaperone CesA regulates Escherichia coli O157:H7 virulence
ABSTRACTEnterohemorrhagic Escherichia coli (EHEC) O157:H7 is a common food-borne pathogen that can cause acute diseases. Lysine acetylation is a post-translational modification (PTM) that occurs in various prokaryotes and is regulated by CobB, the only deacetylase found in bacteria. Here, we demonst...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2024-12-01
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| Series: | Gut Microbes |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19490976.2024.2331435 |
| _version_ | 1827314298878164992 |
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| author | Linxing Li Bin Yang Jing Wang Yi Wei Binbin Xiang Yutao Liu Pan Wu Wanwu Li Yanling Wang Xinyu Zhao Jingliang Qin Miaomiao Liu Ruiying Liu Guozhen Ma Tian Fu Min Wang Bin Liu |
| author_facet | Linxing Li Bin Yang Jing Wang Yi Wei Binbin Xiang Yutao Liu Pan Wu Wanwu Li Yanling Wang Xinyu Zhao Jingliang Qin Miaomiao Liu Ruiying Liu Guozhen Ma Tian Fu Min Wang Bin Liu |
| author_sort | Linxing Li |
| collection | DOAJ |
| description | ABSTRACTEnterohemorrhagic Escherichia coli (EHEC) O157:H7 is a common food-borne pathogen that can cause acute diseases. Lysine acetylation is a post-translational modification (PTM) that occurs in various prokaryotes and is regulated by CobB, the only deacetylase found in bacteria. Here, we demonstrated that CobB plays an important role in the virulence of EHEC O157:H7 and that deletion of cobB significantly decreased the intestinal colonization ability of bacteria. Using acetylation proteomic studies, we systematically identified several proteins that could be regulated by CobB in EHEC O157:H7. Among these CobB substrates, we found that acetylation at the K44 site of CesA, a chaperone for the type-III secretion system (T3SS) translocator protein EspA, weakens its binding to EspA, thereby reducing the stability of this virulence factor; this PTM ultimately attenuating the virulence of EHEC O157:H7. Furthermore, we showed that deacetylation of the K44 site, which is deacetylated by CobB, promotes the interaction between CesA and EspA, thereby increasing bacterial virulence in vitro and in animal experiments. In summary, we showed that acetylation influences the virulence of EHEC O157:H7, and uncovered the mechanism by which CobB contributes to bacterial virulence based on the regulation of CesA deacetylation. |
| first_indexed | 2024-04-24T22:33:05Z |
| format | Article |
| id | doaj.art-d43f8e79448542f3847f6fb3e899cb87 |
| institution | Directory Open Access Journal |
| issn | 1949-0976 1949-0984 |
| language | English |
| last_indexed | 2024-04-24T22:33:05Z |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Gut Microbes |
| spelling | doaj.art-d43f8e79448542f3847f6fb3e899cb872024-03-19T15:53:56ZengTaylor & Francis GroupGut Microbes1949-09761949-09842024-12-0116110.1080/19490976.2024.2331435CobB-mediated deacetylation of the chaperone CesA regulates Escherichia coli O157:H7 virulenceLinxing Li0Bin Yang1Jing Wang2Yi Wei3Binbin Xiang4Yutao Liu5Pan Wu6Wanwu Li7Yanling Wang8Xinyu Zhao9Jingliang Qin10Miaomiao Liu11Ruiying Liu12Guozhen Ma13Tian Fu14Min Wang15Bin Liu16National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, ChinaABSTRACTEnterohemorrhagic Escherichia coli (EHEC) O157:H7 is a common food-borne pathogen that can cause acute diseases. Lysine acetylation is a post-translational modification (PTM) that occurs in various prokaryotes and is regulated by CobB, the only deacetylase found in bacteria. Here, we demonstrated that CobB plays an important role in the virulence of EHEC O157:H7 and that deletion of cobB significantly decreased the intestinal colonization ability of bacteria. Using acetylation proteomic studies, we systematically identified several proteins that could be regulated by CobB in EHEC O157:H7. Among these CobB substrates, we found that acetylation at the K44 site of CesA, a chaperone for the type-III secretion system (T3SS) translocator protein EspA, weakens its binding to EspA, thereby reducing the stability of this virulence factor; this PTM ultimately attenuating the virulence of EHEC O157:H7. Furthermore, we showed that deacetylation of the K44 site, which is deacetylated by CobB, promotes the interaction between CesA and EspA, thereby increasing bacterial virulence in vitro and in animal experiments. In summary, we showed that acetylation influences the virulence of EHEC O157:H7, and uncovered the mechanism by which CobB contributes to bacterial virulence based on the regulation of CesA deacetylation.https://www.tandfonline.com/doi/10.1080/19490976.2024.2331435CobBdeacetylationCesAEspAvirulenceEHEC |
| spellingShingle | Linxing Li Bin Yang Jing Wang Yi Wei Binbin Xiang Yutao Liu Pan Wu Wanwu Li Yanling Wang Xinyu Zhao Jingliang Qin Miaomiao Liu Ruiying Liu Guozhen Ma Tian Fu Min Wang Bin Liu CobB-mediated deacetylation of the chaperone CesA regulates Escherichia coli O157:H7 virulence Gut Microbes CobB deacetylation CesA EspA virulence EHEC |
| title | CobB-mediated deacetylation of the chaperone CesA regulates Escherichia coli O157:H7 virulence |
| title_full | CobB-mediated deacetylation of the chaperone CesA regulates Escherichia coli O157:H7 virulence |
| title_fullStr | CobB-mediated deacetylation of the chaperone CesA regulates Escherichia coli O157:H7 virulence |
| title_full_unstemmed | CobB-mediated deacetylation of the chaperone CesA regulates Escherichia coli O157:H7 virulence |
| title_short | CobB-mediated deacetylation of the chaperone CesA regulates Escherichia coli O157:H7 virulence |
| title_sort | cobb mediated deacetylation of the chaperone cesa regulates escherichia coli o157 h7 virulence |
| topic | CobB deacetylation CesA EspA virulence EHEC |
| url | https://www.tandfonline.com/doi/10.1080/19490976.2024.2331435 |
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