Transcription factor CBF-1 is critical for circadian gene expression by modulating WHITE COLLAR complex recruitment to the frq locus.
Transcription of the Neurospora crassa circadian clock gene frequency (frq) is an essential process in the negative feedback loop that controls circadian rhythms. WHITE COLLAR 1 (WC-1) and WHITE COLLAR 2 (WC-2) forms the WC complex (WCC) that is the main activator of frq transcription by binding to...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2018-09-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC6152987?pdf=render |
| _version_ | 1811268678562349056 |
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| author | Xuemei Cao Xiao Liu Hongda Li Yumeng Fan Jiabin Duan Yi Liu Qun He |
| author_facet | Xuemei Cao Xiao Liu Hongda Li Yumeng Fan Jiabin Duan Yi Liu Qun He |
| author_sort | Xuemei Cao |
| collection | DOAJ |
| description | Transcription of the Neurospora crassa circadian clock gene frequency (frq) is an essential process in the negative feedback loop that controls circadian rhythms. WHITE COLLAR 1 (WC-1) and WHITE COLLAR 2 (WC-2) forms the WC complex (WCC) that is the main activator of frq transcription by binding to its promoter. Here, we show that Centromere Binding Factor 1 (CBF-1) is a critical component of the N. crassa circadian clock by regulating frq transcription. Deletion of cbf-1 resulted in long period and low amplitude rhythms, whereas overexpression of CBF-1 abolished the circadian rhythms. Loss of CBF-1 resulted in WC-independent FRQ expression and suppression of WCC activity. As WCC, CBF-1 also binds to the C-box at the frq promoter. Overexpression of CBF-1 impaired WCC binding to the C-box to suppress frq transcription. Together, our results suggest that the proper level of CBF-1 is critical for circadian clock function by suppressing WC-independent FRQ expression and by regulating WCC binding to the frq promoter. |
| first_indexed | 2024-04-12T21:27:11Z |
| format | Article |
| id | doaj.art-d442edf585d748389153bf6ed68ac9ad |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-04-12T21:27:11Z |
| publishDate | 2018-09-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS Genetics |
| spelling | doaj.art-d442edf585d748389153bf6ed68ac9ad2022-12-22T03:16:08ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042018-09-01149e100757010.1371/journal.pgen.1007570Transcription factor CBF-1 is critical for circadian gene expression by modulating WHITE COLLAR complex recruitment to the frq locus.Xuemei CaoXiao LiuHongda LiYumeng FanJiabin DuanYi LiuQun HeTranscription of the Neurospora crassa circadian clock gene frequency (frq) is an essential process in the negative feedback loop that controls circadian rhythms. WHITE COLLAR 1 (WC-1) and WHITE COLLAR 2 (WC-2) forms the WC complex (WCC) that is the main activator of frq transcription by binding to its promoter. Here, we show that Centromere Binding Factor 1 (CBF-1) is a critical component of the N. crassa circadian clock by regulating frq transcription. Deletion of cbf-1 resulted in long period and low amplitude rhythms, whereas overexpression of CBF-1 abolished the circadian rhythms. Loss of CBF-1 resulted in WC-independent FRQ expression and suppression of WCC activity. As WCC, CBF-1 also binds to the C-box at the frq promoter. Overexpression of CBF-1 impaired WCC binding to the C-box to suppress frq transcription. Together, our results suggest that the proper level of CBF-1 is critical for circadian clock function by suppressing WC-independent FRQ expression and by regulating WCC binding to the frq promoter.http://europepmc.org/articles/PMC6152987?pdf=render |
| spellingShingle | Xuemei Cao Xiao Liu Hongda Li Yumeng Fan Jiabin Duan Yi Liu Qun He Transcription factor CBF-1 is critical for circadian gene expression by modulating WHITE COLLAR complex recruitment to the frq locus. PLoS Genetics |
| title | Transcription factor CBF-1 is critical for circadian gene expression by modulating WHITE COLLAR complex recruitment to the frq locus. |
| title_full | Transcription factor CBF-1 is critical for circadian gene expression by modulating WHITE COLLAR complex recruitment to the frq locus. |
| title_fullStr | Transcription factor CBF-1 is critical for circadian gene expression by modulating WHITE COLLAR complex recruitment to the frq locus. |
| title_full_unstemmed | Transcription factor CBF-1 is critical for circadian gene expression by modulating WHITE COLLAR complex recruitment to the frq locus. |
| title_short | Transcription factor CBF-1 is critical for circadian gene expression by modulating WHITE COLLAR complex recruitment to the frq locus. |
| title_sort | transcription factor cbf 1 is critical for circadian gene expression by modulating white collar complex recruitment to the frq locus |
| url | http://europepmc.org/articles/PMC6152987?pdf=render |
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