CRISPR-Cas-Integrated LAMP
Pathogen-specific point-of-care (PoC) diagnostic tests have become an important need in the fight against infectious diseases and epidemics in recent years. PoC diagnostic tests are designed with the following parameters in mind: rapidity, accuracy, sensitivity, specificity, and ease of use. Molecul...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/12/11/1035 |
| _version_ | 1827644866881912832 |
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| author | Nazente Atçeken Defne Yigci Berin Ozdalgic Savas Tasoglu |
| author_facet | Nazente Atçeken Defne Yigci Berin Ozdalgic Savas Tasoglu |
| author_sort | Nazente Atçeken |
| collection | DOAJ |
| description | Pathogen-specific point-of-care (PoC) diagnostic tests have become an important need in the fight against infectious diseases and epidemics in recent years. PoC diagnostic tests are designed with the following parameters in mind: rapidity, accuracy, sensitivity, specificity, and ease of use. Molecular techniques are the gold standard for pathogen detection due to their accuracy and specificity. There are various limitations in adapting molecular diagnostic methods to PoC diagnostic tests. Efforts to overcome limitations are focused on the development of integrated molecular diagnostics by utilizing the latest technologies available to create the most successful PoC diagnostic platforms. With this point of view, a new generation technology was developed by combining loop-mediated isothermal amplification (LAMP) technology with clustered regularly interspaced short palindromic repeat (CRISPR)-associated (CRISPR-Cas) technology. This integrated approach benefits from the properties of LAMP technology, namely its high efficiency, short turnaround time, and the lack of need for a complex device. It also makes use of the programmable function of CRISPR-Cas technology and the collateral cleavage activity of certain Cas proteins that allow for convenient reporter detection. Thus, this combined technology enables the development of PoC diagnostic tests with high sensitivity, specificity, and ease of use without the need for complicated devices. In this review, we discuss the advantages and limitations of the CRISPR/Cas combined LAMP technology. We review current limitations to convert CRISPR combined LAMP into pathogen-specific PoC platforms. Furthermore, we point out the need to design more useful PoC platforms using microfabrication technologies by developing strategies that overcome the limitations of this new technology, reduce its complexity, and reduce the risk of contamination. |
| first_indexed | 2024-03-09T18:27:47Z |
| format | Article |
| id | doaj.art-d4473b5692d543b4b399574b82355698 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T18:27:47Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-d4473b5692d543b4b399574b823556982023-11-24T07:48:56ZengMDPI AGBiosensors2079-63742022-11-011211103510.3390/bios12111035CRISPR-Cas-Integrated LAMPNazente Atçeken0Defne Yigci1Berin Ozdalgic2Savas Tasoglu3Koç University Translational Medicine Research Center (KUTTAM), Koç University, Istanbul 34450, TurkeySchool of Medicine, Koç University, Istanbul 34450, TurkeyKoç University Translational Medicine Research Center (KUTTAM), Koç University, Istanbul 34450, TurkeyKoç University Translational Medicine Research Center (KUTTAM), Koç University, Istanbul 34450, TurkeyPathogen-specific point-of-care (PoC) diagnostic tests have become an important need in the fight against infectious diseases and epidemics in recent years. PoC diagnostic tests are designed with the following parameters in mind: rapidity, accuracy, sensitivity, specificity, and ease of use. Molecular techniques are the gold standard for pathogen detection due to their accuracy and specificity. There are various limitations in adapting molecular diagnostic methods to PoC diagnostic tests. Efforts to overcome limitations are focused on the development of integrated molecular diagnostics by utilizing the latest technologies available to create the most successful PoC diagnostic platforms. With this point of view, a new generation technology was developed by combining loop-mediated isothermal amplification (LAMP) technology with clustered regularly interspaced short palindromic repeat (CRISPR)-associated (CRISPR-Cas) technology. This integrated approach benefits from the properties of LAMP technology, namely its high efficiency, short turnaround time, and the lack of need for a complex device. It also makes use of the programmable function of CRISPR-Cas technology and the collateral cleavage activity of certain Cas proteins that allow for convenient reporter detection. Thus, this combined technology enables the development of PoC diagnostic tests with high sensitivity, specificity, and ease of use without the need for complicated devices. In this review, we discuss the advantages and limitations of the CRISPR/Cas combined LAMP technology. We review current limitations to convert CRISPR combined LAMP into pathogen-specific PoC platforms. Furthermore, we point out the need to design more useful PoC platforms using microfabrication technologies by developing strategies that overcome the limitations of this new technology, reduce its complexity, and reduce the risk of contamination.https://www.mdpi.com/2079-6374/12/11/1035loop-mediated isothermal amplification (LAMP)clustered regularly interspaced short palindromic repeat (CRISPR)-associated (CRISPR-Cas)CRISPR/Cas combined LAMP technologypoint-of-care (PoC) platform |
| spellingShingle | Nazente Atçeken Defne Yigci Berin Ozdalgic Savas Tasoglu CRISPR-Cas-Integrated LAMP Biosensors loop-mediated isothermal amplification (LAMP) clustered regularly interspaced short palindromic repeat (CRISPR)-associated (CRISPR-Cas) CRISPR/Cas combined LAMP technology point-of-care (PoC) platform |
| title | CRISPR-Cas-Integrated LAMP |
| title_full | CRISPR-Cas-Integrated LAMP |
| title_fullStr | CRISPR-Cas-Integrated LAMP |
| title_full_unstemmed | CRISPR-Cas-Integrated LAMP |
| title_short | CRISPR-Cas-Integrated LAMP |
| title_sort | crispr cas integrated lamp |
| topic | loop-mediated isothermal amplification (LAMP) clustered regularly interspaced short palindromic repeat (CRISPR)-associated (CRISPR-Cas) CRISPR/Cas combined LAMP technology point-of-care (PoC) platform |
| url | https://www.mdpi.com/2079-6374/12/11/1035 |
| work_keys_str_mv | AT nazenteatceken crisprcasintegratedlamp AT defneyigci crisprcasintegratedlamp AT berinozdalgic crisprcasintegratedlamp AT savastasoglu crisprcasintegratedlamp |