Comprehensive evaluation of an one pot quadruplex RT-qPCR assay for the simultaneously subtyping influenza A virus into H1, H3, N1 and N2
In the current study, an one pot quadruplex RT-qPCR assay was established and comprehensively evaluated. The assay's limit of detection could reach as low as 101 copies/reaction, with good repeatability profile and no cross-reaction with other respiratory pathogens. During clinical evaluation b...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-11-01
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| Series: | Journal of Clinical Virology Plus |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667038022000588 |
| _version_ | 1811187738552041472 |
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| author | Hai-Bo Wang Ji-Can Lin Hui-Na Huang Jun-Ting Xu Cheng-Ning Tu |
| author_facet | Hai-Bo Wang Ji-Can Lin Hui-Na Huang Jun-Ting Xu Cheng-Ning Tu |
| author_sort | Hai-Bo Wang |
| collection | DOAJ |
| description | In the current study, an one pot quadruplex RT-qPCR assay was established and comprehensively evaluated. The assay's limit of detection could reach as low as 101 copies/reaction, with good repeatability profile and no cross-reaction with other respiratory pathogens. During clinical evaluation both by blinded samples and real clinical samples, the assay exhibited a 100% coincidence rate with individual commercial RT-qPCR assays. To the best of our knowledge, this is the first report on the simultaneous subtyping influenza A virus into H1, H3, N1, and N2 by one pot quadruplex RT-qPCR assay, which could improve the preparedness for future influenza outbreaks. |
| first_indexed | 2024-04-11T14:08:36Z |
| format | Article |
| id | doaj.art-d44910c17c474a2395567b1f8d53238e |
| institution | Directory Open Access Journal |
| issn | 2667-0380 |
| language | English |
| last_indexed | 2024-04-11T14:08:36Z |
| publishDate | 2022-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Clinical Virology Plus |
| spelling | doaj.art-d44910c17c474a2395567b1f8d53238e2022-12-22T04:19:49ZengElsevierJournal of Clinical Virology Plus2667-03802022-11-0124100119Comprehensive evaluation of an one pot quadruplex RT-qPCR assay for the simultaneously subtyping influenza A virus into H1, H3, N1 and N2Hai-Bo Wang0Ji-Can Lin1Hui-Na Huang2Jun-Ting Xu3Cheng-Ning Tu4Corresponding author at: 7/F, Zhuhai International Travel Healthcare Center, No. 133, Qiaoguang Road, Zhuhai 519020, Guangdong, China.; Key Laboratory of Emerging Infectious Disease Detection, Zhuhai International Travel Healthcare Center, Zhuhai 519020, Guangdong, ChinaKey Laboratory of Emerging Infectious Disease Detection, Zhuhai International Travel Healthcare Center, Zhuhai 519020, Guangdong, ChinaKey Laboratory of Emerging Infectious Disease Detection, Zhuhai International Travel Healthcare Center, Zhuhai 519020, Guangdong, ChinaKey Laboratory of Emerging Infectious Disease Detection, Zhuhai International Travel Healthcare Center, Zhuhai 519020, Guangdong, ChinaKey Laboratory of Emerging Infectious Disease Detection, Zhuhai International Travel Healthcare Center, Zhuhai 519020, Guangdong, ChinaIn the current study, an one pot quadruplex RT-qPCR assay was established and comprehensively evaluated. The assay's limit of detection could reach as low as 101 copies/reaction, with good repeatability profile and no cross-reaction with other respiratory pathogens. During clinical evaluation both by blinded samples and real clinical samples, the assay exhibited a 100% coincidence rate with individual commercial RT-qPCR assays. To the best of our knowledge, this is the first report on the simultaneous subtyping influenza A virus into H1, H3, N1, and N2 by one pot quadruplex RT-qPCR assay, which could improve the preparedness for future influenza outbreaks.http://www.sciencedirect.com/science/article/pii/S2667038022000588Influenza virusSubtypingquadruplex RT-qPCR |
| spellingShingle | Hai-Bo Wang Ji-Can Lin Hui-Na Huang Jun-Ting Xu Cheng-Ning Tu Comprehensive evaluation of an one pot quadruplex RT-qPCR assay for the simultaneously subtyping influenza A virus into H1, H3, N1 and N2 Journal of Clinical Virology Plus Influenza virus Subtyping quadruplex RT-qPCR |
| title | Comprehensive evaluation of an one pot quadruplex RT-qPCR assay for the simultaneously subtyping influenza A virus into H1, H3, N1 and N2 |
| title_full | Comprehensive evaluation of an one pot quadruplex RT-qPCR assay for the simultaneously subtyping influenza A virus into H1, H3, N1 and N2 |
| title_fullStr | Comprehensive evaluation of an one pot quadruplex RT-qPCR assay for the simultaneously subtyping influenza A virus into H1, H3, N1 and N2 |
| title_full_unstemmed | Comprehensive evaluation of an one pot quadruplex RT-qPCR assay for the simultaneously subtyping influenza A virus into H1, H3, N1 and N2 |
| title_short | Comprehensive evaluation of an one pot quadruplex RT-qPCR assay for the simultaneously subtyping influenza A virus into H1, H3, N1 and N2 |
| title_sort | comprehensive evaluation of an one pot quadruplex rt qpcr assay for the simultaneously subtyping influenza a virus into h1 h3 n1 and n2 |
| topic | Influenza virus Subtyping quadruplex RT-qPCR |
| url | http://www.sciencedirect.com/science/article/pii/S2667038022000588 |
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