Comprehensive evaluation of an one pot quadruplex RT-qPCR assay for the simultaneously subtyping influenza A virus into H1, H3, N1 and N2

In the current study, an one pot quadruplex RT-qPCR assay was established and comprehensively evaluated. The assay's limit of detection could reach as low as 101 copies/reaction, with good repeatability profile and no cross-reaction with other respiratory pathogens. During clinical evaluation b...

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Bibliographic Details
Main Authors: Hai-Bo Wang, Ji-Can Lin, Hui-Na Huang, Jun-Ting Xu, Cheng-Ning Tu
Format: Article
Language:English
Published: Elsevier 2022-11-01
Series:Journal of Clinical Virology Plus
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2667038022000588