Comprehensive evaluation of an one pot quadruplex RT-qPCR assay for the simultaneously subtyping influenza A virus into H1, H3, N1 and N2
In the current study, an one pot quadruplex RT-qPCR assay was established and comprehensively evaluated. The assay's limit of detection could reach as low as 101 copies/reaction, with good repeatability profile and no cross-reaction with other respiratory pathogens. During clinical evaluation b...
Main Authors: | Hai-Bo Wang, Ji-Can Lin, Hui-Na Huang, Jun-Ting Xu, Cheng-Ning Tu |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2022-11-01
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Series: | Journal of Clinical Virology Plus |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2667038022000588 |
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