Changes in the triglyceride metabolism in the thigh muscle and liver of broilers injected intravenously with angiopoietin-like protein 4
Angiopoietin-like protein 4 (ANGPTL4) is a potential circulating mediator connecting nutritional factors and fat metabolism, however, information is lacking on the exact role of ANGPTL4 on triglyceride metabolism in the thigh muscles and livers of broilers. The objective of this study was to determi...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2022-09-01
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| Series: | Czech Journal of Animal Science |
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| Online Access: | https://cjas.agriculturejournals.cz/artkey/cjs-202209-0004_changes-in-the-triglyceride-metabolism-in-the-thigh-muscle-and-liver-of-broilers-injected-intravenously-with-an.php |
| _version_ | 1797899154040553472 |
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| author | Xu Zhao Huashan Huang |
| author_facet | Xu Zhao Huashan Huang |
| author_sort | Xu Zhao |
| collection | DOAJ |
| description | Angiopoietin-like protein 4 (ANGPTL4) is a potential circulating mediator connecting nutritional factors and fat metabolism, however, information is lacking on the exact role of ANGPTL4 on triglyceride metabolism in the thigh muscles and livers of broilers. The objective of this study was to determine the changes in the triglyceride metabolism in the thigh muscles and livers of broilers injected intravenously with ANGPTL4. In experiment 1, 36 male Arbor Acres broilers at 35 days of age were randomly allocated into six treatments with six replicates. The broilers were subjected to intravenous injection of polyhistidine-small ubiquitin-related modifier-ANGPTL4 (His-SUMO-ANGPTL4) once at a dose of 0, 20, 100, 500, 2 500, or 12 500 ng/kg body weight (BW), respectively. The results showed that the injection of His-SUMO-ANGPTL4 at a dose of 500, 2 500 and 12 500 ng/kg BW decreased (P < 0.05) the broilers' heart-fatty acid-binding protein (H-FABP) mRNA expression in the thigh muscle. All of the His-SUMO-ANGPTL4 broiler injected groups had a lower (P < 0.05) adipocyte-fatty acid-binding protein mRNA expression in the thigh muscle. In experiment 2, 18 male Arbor Acres broilers at 35 days of age were randomly allocated into three treatments with six replicates. The broilers were given an injection of normal saline (NS), His-SUMO, or His-SUMO-ANGPTL4 (500 ng/kg BW) once. The results showed that the broilers receiving an injection of ANGPTL4 at a dose of 500 ng/kg BW decreased (P < 0.05) the H-FABP mRNA expression in the thigh muscle. Besides, the broilers given an injection of ANGPTL4 at a dose of 500 ng/kg BW increased (P < 0.05) the fatty acid synthase mRNA expression and activity and decreased (P < 0.05) the microsomal triglyceride transfer protein mRNA expression in the liver. However, the concentrations of the high-density lipoprotein, low-density lipoprotein, very low-density lipoprotein, insulin, growth hormone and leptin in the serum were not affected by the ANGPTL4 injection. In conclusion, ANGPTL4 has the ability to change the triglyceride metabolism in the thigh muscles and livers of broilers. |
| first_indexed | 2024-04-10T08:24:25Z |
| format | Article |
| id | doaj.art-d44d7f3cb6dd4670be1c9ff7dac6475c |
| institution | Directory Open Access Journal |
| issn | 1212-1819 1805-9309 |
| language | English |
| last_indexed | 2024-04-10T08:24:25Z |
| publishDate | 2022-09-01 |
| publisher | Czech Academy of Agricultural Sciences |
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| series | Czech Journal of Animal Science |
| spelling | doaj.art-d44d7f3cb6dd4670be1c9ff7dac6475c2023-02-23T03:33:56ZengCzech Academy of Agricultural SciencesCzech Journal of Animal Science1212-18191805-93092022-09-0167937438310.17221/134/2021-CJAScjs-202209-0004Changes in the triglyceride metabolism in the thigh muscle and liver of broilers injected intravenously with angiopoietin-like protein 4Xu Zhao0Huashan Huang1College of Agriculture and Forestry Science, Linyi University, Linyi, P.R. ChinaShandong Longda Biotechnology Co., Ltd, Linyi, P.R. ChinaAngiopoietin-like protein 4 (ANGPTL4) is a potential circulating mediator connecting nutritional factors and fat metabolism, however, information is lacking on the exact role of ANGPTL4 on triglyceride metabolism in the thigh muscles and livers of broilers. The objective of this study was to determine the changes in the triglyceride metabolism in the thigh muscles and livers of broilers injected intravenously with ANGPTL4. In experiment 1, 36 male Arbor Acres broilers at 35 days of age were randomly allocated into six treatments with six replicates. The broilers were subjected to intravenous injection of polyhistidine-small ubiquitin-related modifier-ANGPTL4 (His-SUMO-ANGPTL4) once at a dose of 0, 20, 100, 500, 2 500, or 12 500 ng/kg body weight (BW), respectively. The results showed that the injection of His-SUMO-ANGPTL4 at a dose of 500, 2 500 and 12 500 ng/kg BW decreased (P < 0.05) the broilers' heart-fatty acid-binding protein (H-FABP) mRNA expression in the thigh muscle. All of the His-SUMO-ANGPTL4 broiler injected groups had a lower (P < 0.05) adipocyte-fatty acid-binding protein mRNA expression in the thigh muscle. In experiment 2, 18 male Arbor Acres broilers at 35 days of age were randomly allocated into three treatments with six replicates. The broilers were given an injection of normal saline (NS), His-SUMO, or His-SUMO-ANGPTL4 (500 ng/kg BW) once. The results showed that the broilers receiving an injection of ANGPTL4 at a dose of 500 ng/kg BW decreased (P < 0.05) the H-FABP mRNA expression in the thigh muscle. Besides, the broilers given an injection of ANGPTL4 at a dose of 500 ng/kg BW increased (P < 0.05) the fatty acid synthase mRNA expression and activity and decreased (P < 0.05) the microsomal triglyceride transfer protein mRNA expression in the liver. However, the concentrations of the high-density lipoprotein, low-density lipoprotein, very low-density lipoprotein, insulin, growth hormone and leptin in the serum were not affected by the ANGPTL4 injection. In conclusion, ANGPTL4 has the ability to change the triglyceride metabolism in the thigh muscles and livers of broilers.https://cjas.agriculturejournals.cz/artkey/cjs-202209-0004_changes-in-the-triglyceride-metabolism-in-the-thigh-muscle-and-liver-of-broilers-injected-intravenously-with-an.phpbiochemical parameterschickenhormonelipid metabolism-related enzymerecombinant chicken angiopoietin-like protein 4 |
| spellingShingle | Xu Zhao Huashan Huang Changes in the triglyceride metabolism in the thigh muscle and liver of broilers injected intravenously with angiopoietin-like protein 4 Czech Journal of Animal Science biochemical parameters chicken hormone lipid metabolism-related enzyme recombinant chicken angiopoietin-like protein 4 |
| title | Changes in the triglyceride metabolism in the thigh muscle and liver of broilers injected intravenously with angiopoietin-like protein 4 |
| title_full | Changes in the triglyceride metabolism in the thigh muscle and liver of broilers injected intravenously with angiopoietin-like protein 4 |
| title_fullStr | Changes in the triglyceride metabolism in the thigh muscle and liver of broilers injected intravenously with angiopoietin-like protein 4 |
| title_full_unstemmed | Changes in the triglyceride metabolism in the thigh muscle and liver of broilers injected intravenously with angiopoietin-like protein 4 |
| title_short | Changes in the triglyceride metabolism in the thigh muscle and liver of broilers injected intravenously with angiopoietin-like protein 4 |
| title_sort | changes in the triglyceride metabolism in the thigh muscle and liver of broilers injected intravenously with angiopoietin like protein 4 |
| topic | biochemical parameters chicken hormone lipid metabolism-related enzyme recombinant chicken angiopoietin-like protein 4 |
| url | https://cjas.agriculturejournals.cz/artkey/cjs-202209-0004_changes-in-the-triglyceride-metabolism-in-the-thigh-muscle-and-liver-of-broilers-injected-intravenously-with-an.php |
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