Inactivated vaccine against Aujeszky's disease
Background and Aim: The Aujeszky's disease, also known as Pseudorabies, remains one of the most problematic fulminant diseases in domestic animals, affecting the central nervous system. The study aimed to investigate the effect of an inactivated vaccine against Aujeszky's disease based on...
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| Format: | Article |
| Language: | English |
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Veterinary World
2021-11-01
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| Series: | Veterinary World |
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| Online Access: | http://www.veterinaryworld.org/Vol.14/November-2021/16.pdf |
| _version_ | 1818567343471591424 |
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| author | Zhanat B. Kondibaeva Bolat A. Yespembetov Khairulla B. Abeuov Assiya K. Mussayeva Sarsenbek T. Siyabekov Saltanat T. Nussupova Elmira K. Akmatova Yerlan K. Pazylov Kydyrbay T. Maikhin Nazym S. Syrym |
| author_facet | Zhanat B. Kondibaeva Bolat A. Yespembetov Khairulla B. Abeuov Assiya K. Mussayeva Sarsenbek T. Siyabekov Saltanat T. Nussupova Elmira K. Akmatova Yerlan K. Pazylov Kydyrbay T. Maikhin Nazym S. Syrym |
| author_sort | Zhanat B. Kondibaeva |
| collection | DOAJ |
| description | Background and Aim: The Aujeszky's disease, also known as Pseudorabies, remains one of the most problematic fulminant diseases in domestic animals, affecting the central nervous system. The study aimed to investigate the effect of an inactivated vaccine against Aujeszky's disease based on "Kordai" virus strain.
Materials and Methods: To test the inactivation of the "Kordai" strain (grown by the roller method in VNK-21/13 cell culture with an infectious titer of at least 7.5 lg TCD50/ml) which is causative of Aujeszky's disease, next-generation teotropin and propolis preparations were used in concentrations of 0.1%, 0.08%, and 0.04%.
Results: As a result of comparative studies on the optimization of parameters for inactivating the "Kordai" virus strain, it was established that teotropin is a more effective inactivant than propolis. At the same time, the optimal final concentration of teotropin for inactivation was 0.1%, along with a reaction medium temperature of 37°C, pH of 7.4-7.6, and duration of inactivation of 14 h. The titer of virus-neutralizing activity (VNA) of antibodies at the pH (neutralization reactions) in vaccinated sheep of 10-12 months of age was 7.5±0.3, Ig TCID50/ml (tissue culture infectious dose 50%), and 3.5±0.3 in the cell culture VNK-21/13 (culture of Syrian hamster kidney cells).
Conclusion: To determine colostral immunity in newborn lambs, the method of metabolic status correction was used to vaccinate lambs obtained from immune sheep 4 months after birth. The results showed that lambs obtained from immune sheep had high VNA titers. A sustained immune response in vaccinated animals was obtained after double vaccination. |
| first_indexed | 2024-12-14T06:22:10Z |
| format | Article |
| id | doaj.art-d44e9a47b5f849f1ac97587bc5aa32bf |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-14T06:22:10Z |
| publishDate | 2021-11-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-d44e9a47b5f849f1ac97587bc5aa32bf2022-12-21T23:13:47ZengVeterinary WorldVeterinary World0972-89882231-09162021-11-0114112957296310.14202/vetworld.2021.2957-2963Inactivated vaccine against Aujeszky's diseaseZhanat B. Kondibaeva0https://orcid.org/0000-0002-2959-1161Bolat A. Yespembetov1https://orcid.org/0000-0002-9116-6836Khairulla B. Abeuov2https://orcid.org/0000-0002-6925-9315Assiya K. Mussayeva3https://orcid.org/0000-0001-5476-4207Sarsenbek T. Siyabekov4https://orcid.org/0000-0002-2266-6351Saltanat T. Nussupova5https://orcid.org/0000-0002-9553-0666Elmira K. Akmatova6https://orcid.org/0000-0002-2805-8701Yerlan K. Pazylov7https://orcid.org/0000-0001-6500-668XKydyrbay T. Maikhin8https://orcid.org/0000-0003-1744-2860Nazym S. Syrym9https://orcid.org/0000-0002-7313-0899Laboratory of Diagnostic of the Infectious Diseases , Research Institute for Biological Safety Problems, Gwardeiski, Kazakhstan.Laboratory of Microbiology , Research Institute for Biological Safety Problems, Gwardeiski, Kazakhstan.Laboratory of Diagnostic of the Infectious Diseases , Research Institute for Biological Safety Problems, Gwardeiski, Kazakhstan.Bacteriology Laboratory , Kazakh Scientific Research Veterinary Institute, Astana, Kazakhstan.Department of Clinical Veterinary Medicine , Kazakh National Agrarian University, Almaty, Kazakhstan.Department of Clinical Veterinary Medicine , Kazakh National Agrarian University, Almaty, Kazakhstan.Laboratory o the diseases of domestic animals, Kyrgyz Research Institute of Veterinary named after A. Duysheev, Bishkek, Kyrgyzstan.Laboratory of Diagnosis of Infectious Diseases, National Reference Veterinary Center Almaty Branch, Almaty, Kazakhstan.Laboratory of Diagnosis of Infectious Diseases, National Reference Veterinary Center Almaty Branch, Almaty, Kazakhstan.Laboratory of Microbiology , Research Institute for Biological Safety Problems, Gwardeiski, Kazakhstan.Background and Aim: The Aujeszky's disease, also known as Pseudorabies, remains one of the most problematic fulminant diseases in domestic animals, affecting the central nervous system. The study aimed to investigate the effect of an inactivated vaccine against Aujeszky's disease based on "Kordai" virus strain. Materials and Methods: To test the inactivation of the "Kordai" strain (grown by the roller method in VNK-21/13 cell culture with an infectious titer of at least 7.5 lg TCD50/ml) which is causative of Aujeszky's disease, next-generation teotropin and propolis preparations were used in concentrations of 0.1%, 0.08%, and 0.04%. Results: As a result of comparative studies on the optimization of parameters for inactivating the "Kordai" virus strain, it was established that teotropin is a more effective inactivant than propolis. At the same time, the optimal final concentration of teotropin for inactivation was 0.1%, along with a reaction medium temperature of 37°C, pH of 7.4-7.6, and duration of inactivation of 14 h. The titer of virus-neutralizing activity (VNA) of antibodies at the pH (neutralization reactions) in vaccinated sheep of 10-12 months of age was 7.5±0.3, Ig TCID50/ml (tissue culture infectious dose 50%), and 3.5±0.3 in the cell culture VNK-21/13 (culture of Syrian hamster kidney cells). Conclusion: To determine colostral immunity in newborn lambs, the method of metabolic status correction was used to vaccinate lambs obtained from immune sheep 4 months after birth. The results showed that lambs obtained from immune sheep had high VNA titers. A sustained immune response in vaccinated animals was obtained after double vaccination.http://www.veterinaryworld.org/Vol.14/November-2021/16.pdfadjuvantaujeszky's disease viruscolostral immunityimmunogenicitystrain "kordai" |
| spellingShingle | Zhanat B. Kondibaeva Bolat A. Yespembetov Khairulla B. Abeuov Assiya K. Mussayeva Sarsenbek T. Siyabekov Saltanat T. Nussupova Elmira K. Akmatova Yerlan K. Pazylov Kydyrbay T. Maikhin Nazym S. Syrym Inactivated vaccine against Aujeszky's disease Veterinary World adjuvant aujeszky's disease virus colostral immunity immunogenicity strain "kordai" |
| title | Inactivated vaccine against Aujeszky's disease |
| title_full | Inactivated vaccine against Aujeszky's disease |
| title_fullStr | Inactivated vaccine against Aujeszky's disease |
| title_full_unstemmed | Inactivated vaccine against Aujeszky's disease |
| title_short | Inactivated vaccine against Aujeszky's disease |
| title_sort | inactivated vaccine against aujeszky s disease |
| topic | adjuvant aujeszky's disease virus colostral immunity immunogenicity strain "kordai" |
| url | http://www.veterinaryworld.org/Vol.14/November-2021/16.pdf |
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