Comparison of the bactericidal efficacy of photodynamic therapy, 2.5% sodium hypochlorite, and 2% chlorhexidine against Enterococcous faecalis in root canals; an in vitro study
Background : Enterococcus faecalis has been widely used as a valuable microbiological pathogen for in vitro studies due to its ability to successfully colonize the root canal in a biofilm-like style, invade dentinal tubules, and resist endodontic treatment procedures.The aim of this study was to com...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2012-01-01
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| Series: | Dental Research Journal |
| Subjects: | |
| Online Access: | http://www.drjjournal.net/article.asp?issn=1735-3327;year=2012;volume=9;issue=5;spage=613;epage=618;aulast=Vaziri |
| _version_ | 1819092268104024064 |
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| author | Shahram Vaziri Ali Kangarlou Razieh Shahbazi Amin Nazari Nasab Mandana Naseri |
| author_facet | Shahram Vaziri Ali Kangarlou Razieh Shahbazi Amin Nazari Nasab Mandana Naseri |
| author_sort | Shahram Vaziri |
| collection | DOAJ |
| description | Background : Enterococcus faecalis has been widely used as a valuable microbiological pathogen for in vitro studies due to its ability to successfully colonize the root canal in a biofilm-like style, invade dentinal tubules, and resist endodontic treatment procedures.The aim of this study was to compare the bactericidal efficacy of photodynamic therapy (PDT), 2% chlorhexidine, 2.5% NaOCl, and combination of PDT and 2.5% NaOCl against E. faecalis.
Materials and Methods: Sixty single-rooted teeth had their canals contaminated with E. faecalis in brain heart infusion broth and were incubated for 48 hours.The canals were then subjected to 2% chlorhexidine, 2.5% NaOCl, PDT (red light emitting diode 625 nm+ Toludine Blue) and PDT + 2.5% NaOCl. Controls consisted of no treatment (positive control) and without inoculation of bacterium (negative control). Following treatment, the canal contents were sampled with sterile paper points.The samples were dispersed in transport medium, serially diluted, and cultured on blood agar to determine the number of colony forming units (CFU). Data were analyzed by Mann-Whitney U test at 5% significance level. The significance level for all analyses was set at P <.05.
Results: Combination of PDT and 2.5% NaOCl achieved maximum reduction in recovered viable bacteria, no viable bacteria was observed after treatment of PDT + 2.5% NaOCl.
Conclusion: Combination of PDT and 2.5% NaOCl simultaneously is effective in the elimination of E. faecalis from dentinal tubules under the conditions of this study. |
| first_indexed | 2024-12-21T22:52:55Z |
| format | Article |
| id | doaj.art-d44ff62c2bc54ae88708ad40d6e0963f |
| institution | Directory Open Access Journal |
| issn | 1735-3327 2008-0255 |
| language | English |
| last_indexed | 2024-12-21T22:52:55Z |
| publishDate | 2012-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Dental Research Journal |
| spelling | doaj.art-d44ff62c2bc54ae88708ad40d6e0963f2022-12-21T18:47:31ZengWolters Kluwer Medknow PublicationsDental Research Journal1735-33272008-02552012-01-019561361810.4103/1735-3327.104882Comparison of the bactericidal efficacy of photodynamic therapy, 2.5% sodium hypochlorite, and 2% chlorhexidine against Enterococcous faecalis in root canals; an in vitro studyShahram VaziriAli KangarlouRazieh ShahbaziAmin Nazari NasabMandana NaseriBackground : Enterococcus faecalis has been widely used as a valuable microbiological pathogen for in vitro studies due to its ability to successfully colonize the root canal in a biofilm-like style, invade dentinal tubules, and resist endodontic treatment procedures.The aim of this study was to compare the bactericidal efficacy of photodynamic therapy (PDT), 2% chlorhexidine, 2.5% NaOCl, and combination of PDT and 2.5% NaOCl against E. faecalis. Materials and Methods: Sixty single-rooted teeth had their canals contaminated with E. faecalis in brain heart infusion broth and were incubated for 48 hours.The canals were then subjected to 2% chlorhexidine, 2.5% NaOCl, PDT (red light emitting diode 625 nm+ Toludine Blue) and PDT + 2.5% NaOCl. Controls consisted of no treatment (positive control) and without inoculation of bacterium (negative control). Following treatment, the canal contents were sampled with sterile paper points.The samples were dispersed in transport medium, serially diluted, and cultured on blood agar to determine the number of colony forming units (CFU). Data were analyzed by Mann-Whitney U test at 5% significance level. The significance level for all analyses was set at P <.05. Results: Combination of PDT and 2.5% NaOCl achieved maximum reduction in recovered viable bacteria, no viable bacteria was observed after treatment of PDT + 2.5% NaOCl. Conclusion: Combination of PDT and 2.5% NaOCl simultaneously is effective in the elimination of E. faecalis from dentinal tubules under the conditions of this study.http://www.drjjournal.net/article.asp?issn=1735-3327;year=2012;volume=9;issue=5;spage=613;epage=618;aulast=VaziriEnterococcus faecalis sodium hypochloritephotosensitizationroot canalstoluidine blue |
| spellingShingle | Shahram Vaziri Ali Kangarlou Razieh Shahbazi Amin Nazari Nasab Mandana Naseri Comparison of the bactericidal efficacy of photodynamic therapy, 2.5% sodium hypochlorite, and 2% chlorhexidine against Enterococcous faecalis in root canals; an in vitro study Dental Research Journal Enterococcus faecalis sodium hypochlorite photosensitization root canals toluidine blue |
| title | Comparison of the bactericidal efficacy of photodynamic therapy, 2.5% sodium hypochlorite, and 2% chlorhexidine against Enterococcous faecalis in root canals; an in vitro study |
| title_full | Comparison of the bactericidal efficacy of photodynamic therapy, 2.5% sodium hypochlorite, and 2% chlorhexidine against Enterococcous faecalis in root canals; an in vitro study |
| title_fullStr | Comparison of the bactericidal efficacy of photodynamic therapy, 2.5% sodium hypochlorite, and 2% chlorhexidine against Enterococcous faecalis in root canals; an in vitro study |
| title_full_unstemmed | Comparison of the bactericidal efficacy of photodynamic therapy, 2.5% sodium hypochlorite, and 2% chlorhexidine against Enterococcous faecalis in root canals; an in vitro study |
| title_short | Comparison of the bactericidal efficacy of photodynamic therapy, 2.5% sodium hypochlorite, and 2% chlorhexidine against Enterococcous faecalis in root canals; an in vitro study |
| title_sort | comparison of the bactericidal efficacy of photodynamic therapy 2 5 sodium hypochlorite and 2 chlorhexidine against enterococcous faecalis in root canals an in vitro study |
| topic | Enterococcus faecalis sodium hypochlorite photosensitization root canals toluidine blue |
| url | http://www.drjjournal.net/article.asp?issn=1735-3327;year=2012;volume=9;issue=5;spage=613;epage=618;aulast=Vaziri |
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