The anti-caries activity and toxicity of an experimental propolis-containing varnish
Abstract We investigated the anti-caries effects of an experimental propolis varnish in vivo, and further tested its toxicity against fibroblasts. Fifty-six SPF female Wistar rats were infected with Streptococcus mutans UA159 (SM) and allocated into four groups (n = 14/group): G1, propolis varnish (...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Pesquisa Odontológica
2017-06-01
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| Series: | Brazilian Oral Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242017000100242&lng=en&tlng=en |
| _version_ | 1828470589412605952 |
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| author | Mariana Passos DE LUCA Irlan Almeida FREIRES Alfonso GALA-GARCÍA Vagner Rodrigues SANTOS Miriam Pimenta VALE Severino Matias de ALENCAR Pedro Luiz ROSALEN |
| author_facet | Mariana Passos DE LUCA Irlan Almeida FREIRES Alfonso GALA-GARCÍA Vagner Rodrigues SANTOS Miriam Pimenta VALE Severino Matias de ALENCAR Pedro Luiz ROSALEN |
| author_sort | Mariana Passos DE LUCA |
| collection | DOAJ |
| description | Abstract We investigated the anti-caries effects of an experimental propolis varnish in vivo, and further tested its toxicity against fibroblasts. Fifty-six SPF female Wistar rats were infected with Streptococcus mutans UA159 (SM) and allocated into four groups (n = 14/group): G1, propolis varnish (15%/PV); G2, chitosan varnish (CV/vehicle); G3, gold standard (GS/Duraphat®); and G4, untreated. The animals received a single varnish application on their molars and were submitted to a high cariogenic challenge (Diet-2000, 56% sucrose, and 5% sucrose-added water, ad libitum) for 4 weeks. Total cultivable microbiota and SM were counted, and smooth-surface and sulcal caries were scored. PV, CV and GS cytotoxic effects were tested against fibroblasts. The data were analyzed using ANOVA with the Tukey-Kramer test (p ≤ 0.05). Total microbiota and SM counts did not differ among the treatments (p = 0.78), or in relation to the untreated group (p = 0.52). PV reduced development of smooth-surface enamel caries compared with the untreated group (p = 0.0018), with no significant difference from GS (p = 0.92); however, the PV effects were no longer observed when the dentin was affected. Neither PV nor GS prevented enamel sulcal lesion onset, but GS significantly reduced the severity of dentinal sulcal lesions (p < 0.0001). No significant difference was observed in fibroblast viability between PV and GS (p < 0.0001). In conclusion, PV prevented smooth-surface enamel caries and showed low cell toxicity. Nevertheless, due to the high cariogenic challenge, its effects were not sustained throughout the experiment. Further studies are encouraged to establish a protocol to sustain the long-term anti-caries activity of PV in the oral cavity. |
| first_indexed | 2024-12-11T04:56:09Z |
| format | Article |
| id | doaj.art-d45114abd41b43ac8285ee307009d202 |
| institution | Directory Open Access Journal |
| issn | 1807-3107 |
| language | English |
| last_indexed | 2024-12-11T04:56:09Z |
| publishDate | 2017-06-01 |
| publisher | Sociedade Brasileira de Pesquisa Odontológica |
| record_format | Article |
| series | Brazilian Oral Research |
| spelling | doaj.art-d45114abd41b43ac8285ee307009d2022022-12-22T01:20:16ZengSociedade Brasileira de Pesquisa OdontológicaBrazilian Oral Research1807-31072017-06-0131010.1590/1807-3107bor-2017.vol31.0045S1806-83242017000100242The anti-caries activity and toxicity of an experimental propolis-containing varnishMariana Passos DE LUCAIrlan Almeida FREIRESAlfonso GALA-GARCÍAVagner Rodrigues SANTOSMiriam Pimenta VALESeverino Matias de ALENCARPedro Luiz ROSALENAbstract We investigated the anti-caries effects of an experimental propolis varnish in vivo, and further tested its toxicity against fibroblasts. Fifty-six SPF female Wistar rats were infected with Streptococcus mutans UA159 (SM) and allocated into four groups (n = 14/group): G1, propolis varnish (15%/PV); G2, chitosan varnish (CV/vehicle); G3, gold standard (GS/Duraphat®); and G4, untreated. The animals received a single varnish application on their molars and were submitted to a high cariogenic challenge (Diet-2000, 56% sucrose, and 5% sucrose-added water, ad libitum) for 4 weeks. Total cultivable microbiota and SM were counted, and smooth-surface and sulcal caries were scored. PV, CV and GS cytotoxic effects were tested against fibroblasts. The data were analyzed using ANOVA with the Tukey-Kramer test (p ≤ 0.05). Total microbiota and SM counts did not differ among the treatments (p = 0.78), or in relation to the untreated group (p = 0.52). PV reduced development of smooth-surface enamel caries compared with the untreated group (p = 0.0018), with no significant difference from GS (p = 0.92); however, the PV effects were no longer observed when the dentin was affected. Neither PV nor GS prevented enamel sulcal lesion onset, but GS significantly reduced the severity of dentinal sulcal lesions (p < 0.0001). No significant difference was observed in fibroblast viability between PV and GS (p < 0.0001). In conclusion, PV prevented smooth-surface enamel caries and showed low cell toxicity. Nevertheless, due to the high cariogenic challenge, its effects were not sustained throughout the experiment. Further studies are encouraged to establish a protocol to sustain the long-term anti-caries activity of PV in the oral cavity.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242017000100242&lng=en&tlng=enPropolisStreptococcus mutansDental CariesModels, Animal |
| spellingShingle | Mariana Passos DE LUCA Irlan Almeida FREIRES Alfonso GALA-GARCÍA Vagner Rodrigues SANTOS Miriam Pimenta VALE Severino Matias de ALENCAR Pedro Luiz ROSALEN The anti-caries activity and toxicity of an experimental propolis-containing varnish Brazilian Oral Research Propolis Streptococcus mutans Dental Caries Models, Animal |
| title | The anti-caries activity and toxicity of an experimental propolis-containing varnish |
| title_full | The anti-caries activity and toxicity of an experimental propolis-containing varnish |
| title_fullStr | The anti-caries activity and toxicity of an experimental propolis-containing varnish |
| title_full_unstemmed | The anti-caries activity and toxicity of an experimental propolis-containing varnish |
| title_short | The anti-caries activity and toxicity of an experimental propolis-containing varnish |
| title_sort | anti caries activity and toxicity of an experimental propolis containing varnish |
| topic | Propolis Streptococcus mutans Dental Caries Models, Animal |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242017000100242&lng=en&tlng=en |
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