Aluminum Nanoparticles Affect Human Platelet Function In Vitro
Endoprostheses are prone to tribological wear and biological processes that lead to the release of particles, including aluminum nanoparticles (Al NPs). Those particles can diffuse into circulation. However, the toxic effects of NPs on platelets have not been comprehensively analyzed. The aim of our...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/3/2547 |
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| author | Dominik Taterra Bendik Skinningsrud Sigurd Lauritzen Przemysław A. Pękala Dawid Szwedowski Iwona M. Tomaszewska Krzysztof A. Tomaszewski |
| author_facet | Dominik Taterra Bendik Skinningsrud Sigurd Lauritzen Przemysław A. Pękala Dawid Szwedowski Iwona M. Tomaszewska Krzysztof A. Tomaszewski |
| author_sort | Dominik Taterra |
| collection | DOAJ |
| description | Endoprostheses are prone to tribological wear and biological processes that lead to the release of particles, including aluminum nanoparticles (Al NPs). Those particles can diffuse into circulation. However, the toxic effects of NPs on platelets have not been comprehensively analyzed. The aim of our work was to investigate the impact of Al NPs on human platelet function using a novel quartz crystal microbalance with dissipation (QCM-D) methodology. Moreover, a suite of assays, including light transmission aggregometry, flow cytometry, optical microscopy and transmission electron microscopy, were utilized. All Al NPs caused a significant increase in dissipation (D) and frequency (F), indicating platelet aggregation even at the lowest tested concentration (0.5 µg/mL), except for the largest (80 nm) Al NPs. A size-dependent effect on platelet aggregation was observed for the 5–20 nm NPs and the 30–50 nm NPs, with the larger Al NPs causing smaller increases in D and F; however, this was not observed for the 20–30 nm NPs. In conclusion, our study showed that small (5–50 nm) Al NPs caused platelet aggregation, and larger (80 nm) caused a bridging–penetrating effect in entering platelets, resulting in the formation of heterologous platelet–Al NPs structures. Therefore, physicians should consider monitoring NP serum levels and platelet activation indices in patients with orthopedic implants. |
| first_indexed | 2024-03-11T09:41:16Z |
| format | Article |
| id | doaj.art-d45b3d7105d7457f9a35fb764fe5e347 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T09:41:16Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-d45b3d7105d7457f9a35fb764fe5e3472023-11-16T16:58:51ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-01-01243254710.3390/ijms24032547Aluminum Nanoparticles Affect Human Platelet Function In VitroDominik Taterra0Bendik Skinningsrud1Sigurd Lauritzen2Przemysław A. Pękala3Dawid Szwedowski4Iwona M. Tomaszewska5Krzysztof A. Tomaszewski6International Evidence-Based Anatomy Working Group, 30-034 Krakow, PolandInternational Evidence-Based Anatomy Working Group, 30-034 Krakow, PolandInternational Evidence-Based Anatomy Working Group, 30-034 Krakow, PolandInternational Evidence-Based Anatomy Working Group, 30-034 Krakow, PolandDepartment of Organic Chemistry, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, 85-094 Bydgoszcz, PolandDepartment of Medical Education, Jagiellonian University Medical College, 31-034 Krakow, PolandInternational Evidence-Based Anatomy Working Group, 30-034 Krakow, PolandEndoprostheses are prone to tribological wear and biological processes that lead to the release of particles, including aluminum nanoparticles (Al NPs). Those particles can diffuse into circulation. However, the toxic effects of NPs on platelets have not been comprehensively analyzed. The aim of our work was to investigate the impact of Al NPs on human platelet function using a novel quartz crystal microbalance with dissipation (QCM-D) methodology. Moreover, a suite of assays, including light transmission aggregometry, flow cytometry, optical microscopy and transmission electron microscopy, were utilized. All Al NPs caused a significant increase in dissipation (D) and frequency (F), indicating platelet aggregation even at the lowest tested concentration (0.5 µg/mL), except for the largest (80 nm) Al NPs. A size-dependent effect on platelet aggregation was observed for the 5–20 nm NPs and the 30–50 nm NPs, with the larger Al NPs causing smaller increases in D and F; however, this was not observed for the 20–30 nm NPs. In conclusion, our study showed that small (5–50 nm) Al NPs caused platelet aggregation, and larger (80 nm) caused a bridging–penetrating effect in entering platelets, resulting in the formation of heterologous platelet–Al NPs structures. Therefore, physicians should consider monitoring NP serum levels and platelet activation indices in patients with orthopedic implants.https://www.mdpi.com/1422-0067/24/3/2547aluminum nanoparticlesceramic prosthesesplatelet aggregation |
| spellingShingle | Dominik Taterra Bendik Skinningsrud Sigurd Lauritzen Przemysław A. Pękala Dawid Szwedowski Iwona M. Tomaszewska Krzysztof A. Tomaszewski Aluminum Nanoparticles Affect Human Platelet Function In Vitro International Journal of Molecular Sciences aluminum nanoparticles ceramic prostheses platelet aggregation |
| title | Aluminum Nanoparticles Affect Human Platelet Function In Vitro |
| title_full | Aluminum Nanoparticles Affect Human Platelet Function In Vitro |
| title_fullStr | Aluminum Nanoparticles Affect Human Platelet Function In Vitro |
| title_full_unstemmed | Aluminum Nanoparticles Affect Human Platelet Function In Vitro |
| title_short | Aluminum Nanoparticles Affect Human Platelet Function In Vitro |
| title_sort | aluminum nanoparticles affect human platelet function in vitro |
| topic | aluminum nanoparticles ceramic prostheses platelet aggregation |
| url | https://www.mdpi.com/1422-0067/24/3/2547 |
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