Protocol optimization for the detection of Trypanosoma cruzi DNA in açai (Euterpe oleraceae) pulp
ABSTRACT Chagas disease, caused by the protozoan Trypanosoma cruzi, has often been linked to oral transmission through açai consumption. Molecular methods that allow fast and accurate identification of the pathogen are important for the detection of the presence of the parasite in this food. This st...
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| Format: | Article |
| Language: | English |
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Instituto Nacional de Pesquisas da Amazônia
2021-03-01
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| Series: | Acta Amazonica |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672021000100079&tlng=en |
| _version_ | 1819258933524561920 |
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| author | Gabrielle Virgínia Ferreira CARDOSO Andrey Carlos do Sacramento de OLIVEIRA Andréia Silva da SILVA Marcos Clécio de Lemos SILVA Joelson Sousa LIMA Talita Bandeira ROOS Carina Martins de MORAES |
| author_facet | Gabrielle Virgínia Ferreira CARDOSO Andrey Carlos do Sacramento de OLIVEIRA Andréia Silva da SILVA Marcos Clécio de Lemos SILVA Joelson Sousa LIMA Talita Bandeira ROOS Carina Martins de MORAES |
| author_sort | Gabrielle Virgínia Ferreira CARDOSO |
| collection | DOAJ |
| description | ABSTRACT Chagas disease, caused by the protozoan Trypanosoma cruzi, has often been linked to oral transmission through açai consumption. Molecular methods that allow fast and accurate identification of the pathogen are important for the detection of the presence of the parasite in this food. This study aimed to optimize polymerase chain reaction (PCR)-based detection of T. cruzi DNA in açai pulp. Several dilutions of T. cruzi DTU TcI trypomastigote forms were cultured in liver infusion tryptose (LIT) medium. Trypanosoma cruzi DNA was extracted from the cells and subjected to PCR. Subsequently, culture dilutions were added to açai pulp to evaluate the detection threshold of the optimized PCR assay. We demonstrate that our assay can detect T. cruzi DNA in açai pulp at a concentration of 1.08 × 10-10 ng µL-1. We conclude that our optimized protocol is effective and can be used as an important tool for the detection of T. cruzi contamination in açaí. |
| first_indexed | 2024-12-23T19:01:59Z |
| format | Article |
| id | doaj.art-d45e0c648e4a4fb6b21b7d0e540b3784 |
| institution | Directory Open Access Journal |
| issn | 0044-5967 |
| language | English |
| last_indexed | 2024-12-23T19:01:59Z |
| publishDate | 2021-03-01 |
| publisher | Instituto Nacional de Pesquisas da Amazônia |
| record_format | Article |
| series | Acta Amazonica |
| spelling | doaj.art-d45e0c648e4a4fb6b21b7d0e540b37842022-12-21T17:34:41ZengInstituto Nacional de Pesquisas da AmazôniaActa Amazonica0044-59672021-03-01511798410.1590/1809-4392201903426Protocol optimization for the detection of Trypanosoma cruzi DNA in açai (Euterpe oleraceae) pulpGabrielle Virgínia Ferreira CARDOSOhttps://orcid.org/0000-0002-6407-2068Andrey Carlos do Sacramento de OLIVEIRAAndréia Silva da SILVAMarcos Clécio de Lemos SILVAJoelson Sousa LIMATalita Bandeira ROOSCarina Martins de MORAESABSTRACT Chagas disease, caused by the protozoan Trypanosoma cruzi, has often been linked to oral transmission through açai consumption. Molecular methods that allow fast and accurate identification of the pathogen are important for the detection of the presence of the parasite in this food. This study aimed to optimize polymerase chain reaction (PCR)-based detection of T. cruzi DNA in açai pulp. Several dilutions of T. cruzi DTU TcI trypomastigote forms were cultured in liver infusion tryptose (LIT) medium. Trypanosoma cruzi DNA was extracted from the cells and subjected to PCR. Subsequently, culture dilutions were added to açai pulp to evaluate the detection threshold of the optimized PCR assay. We demonstrate that our assay can detect T. cruzi DNA in açai pulp at a concentration of 1.08 × 10-10 ng µL-1. We conclude that our optimized protocol is effective and can be used as an important tool for the detection of T. cruzi contamination in açaí.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672021000100079&tlng=endetection methodfoodborne diseasefood pathogenmolecular detectionChagas disease |
| spellingShingle | Gabrielle Virgínia Ferreira CARDOSO Andrey Carlos do Sacramento de OLIVEIRA Andréia Silva da SILVA Marcos Clécio de Lemos SILVA Joelson Sousa LIMA Talita Bandeira ROOS Carina Martins de MORAES Protocol optimization for the detection of Trypanosoma cruzi DNA in açai (Euterpe oleraceae) pulp Acta Amazonica detection method foodborne disease food pathogen molecular detection Chagas disease |
| title | Protocol optimization for the detection of Trypanosoma cruzi DNA in açai (Euterpe oleraceae) pulp |
| title_full | Protocol optimization for the detection of Trypanosoma cruzi DNA in açai (Euterpe oleraceae) pulp |
| title_fullStr | Protocol optimization for the detection of Trypanosoma cruzi DNA in açai (Euterpe oleraceae) pulp |
| title_full_unstemmed | Protocol optimization for the detection of Trypanosoma cruzi DNA in açai (Euterpe oleraceae) pulp |
| title_short | Protocol optimization for the detection of Trypanosoma cruzi DNA in açai (Euterpe oleraceae) pulp |
| title_sort | protocol optimization for the detection of trypanosoma cruzi dna in acai euterpe oleraceae pulp |
| topic | detection method foodborne disease food pathogen molecular detection Chagas disease |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672021000100079&tlng=en |
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