The metabolic resistance of Nilaparvata lugens to chlorpyrifos is mainly driven by the carboxylesterase CarE17
The involvement of carboxylesterases (CarEs) in resistance to chlorpyrifos has been confirmed by the synergism analysis in Nilaparvata lugens. However, the function of specific CarE gene in chlorpyrifos resistance and the transcriptional regulatory mechanism are obscure. Herein, the expression patte...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-08-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651322005784 |
| _version_ | 1828271457932673024 |
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| author | Kai Lu Yimin Li Tianxiang Xiao Zhongxiang Sun |
| author_facet | Kai Lu Yimin Li Tianxiang Xiao Zhongxiang Sun |
| author_sort | Kai Lu |
| collection | DOAJ |
| description | The involvement of carboxylesterases (CarEs) in resistance to chlorpyrifos has been confirmed by the synergism analysis in Nilaparvata lugens. However, the function of specific CarE gene in chlorpyrifos resistance and the transcriptional regulatory mechanism are obscure. Herein, the expression patterns of 29 CarE genes in the susceptible and chlorpyrifos-resistant strains were analyzed. Among them, CarE3, CarE17 and CarE19 were overexpressed in the resistant strain, and knockdown of either CarE gene by RNA interference significantly increased the susceptibility to chlorpyrifos. Remarkably, knockdown of CarE17 reduced the enzymatic activity of CarE by 88.63 % and showed a much greater effect on increasing chlorpyrifos toxicity than silencing other two CarE genes. Overexpression of CarE17 in Drosophila melanogaster decreased the toxicity of chlorpyrifos to transgenic fruit flies. Furthermore, the region between − 205 to + 256 of CarE17 promoter sequence showed the highest promoter activity, and 16 transcription factors (TFs) were predicted from this region. Among these TFs, Lim1β and C15 were overexpressed in the resistant strain. Knockdown of either TF resulted in reduced CarE17 expression and a decrease in resistance of N. lugens to chlorpyrifos. These results indicate that the constitutive overexpression of Lim1β and C15 induces CarE17 expression thus conferring chlorpyrifos resistance in N. lugens. |
| first_indexed | 2024-04-13T05:53:28Z |
| format | Article |
| id | doaj.art-d46350f132214edd92426d8ec3c63186 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-13T05:53:28Z |
| publishDate | 2022-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-d46350f132214edd92426d8ec3c631862022-12-22T02:59:42ZengElsevierEcotoxicology and Environmental Safety0147-65132022-08-01241113738The metabolic resistance of Nilaparvata lugens to chlorpyrifos is mainly driven by the carboxylesterase CarE17Kai Lu0Yimin Li1Tianxiang Xiao2Zhongxiang Sun3Anhui Province Key Laboratory of Crop Integrated Pest Management, Anhui Province Engineering Laboratory for Green Pesticide Development and Application, School of Plant Protection, Anhui Agricultural University, Hefei 230036, China; College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Corresponding author at: Anhui Province Key Laboratory of Crop Integrated Pest Management, Anhui Province Engineering Laboratory for Green Pesticide Development and Application, School of Plant Protection, Anhui Agricultural University, Hefei 230036, China.College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaAnhui Province Key Laboratory of Crop Integrated Pest Management, Anhui Province Engineering Laboratory for Green Pesticide Development and Application, School of Plant Protection, Anhui Agricultural University, Hefei 230036, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, School of Plant Protection, Yunnan Agricultural University, Kunming 650201, China; Corresponding author.The involvement of carboxylesterases (CarEs) in resistance to chlorpyrifos has been confirmed by the synergism analysis in Nilaparvata lugens. However, the function of specific CarE gene in chlorpyrifos resistance and the transcriptional regulatory mechanism are obscure. Herein, the expression patterns of 29 CarE genes in the susceptible and chlorpyrifos-resistant strains were analyzed. Among them, CarE3, CarE17 and CarE19 were overexpressed in the resistant strain, and knockdown of either CarE gene by RNA interference significantly increased the susceptibility to chlorpyrifos. Remarkably, knockdown of CarE17 reduced the enzymatic activity of CarE by 88.63 % and showed a much greater effect on increasing chlorpyrifos toxicity than silencing other two CarE genes. Overexpression of CarE17 in Drosophila melanogaster decreased the toxicity of chlorpyrifos to transgenic fruit flies. Furthermore, the region between − 205 to + 256 of CarE17 promoter sequence showed the highest promoter activity, and 16 transcription factors (TFs) were predicted from this region. Among these TFs, Lim1β and C15 were overexpressed in the resistant strain. Knockdown of either TF resulted in reduced CarE17 expression and a decrease in resistance of N. lugens to chlorpyrifos. These results indicate that the constitutive overexpression of Lim1β and C15 induces CarE17 expression thus conferring chlorpyrifos resistance in N. lugens.http://www.sciencedirect.com/science/article/pii/S0147651322005784Brown planthopperInsecticide resistanceMetabolic detoxificationHomeoboxRegulatory mechanism |
| spellingShingle | Kai Lu Yimin Li Tianxiang Xiao Zhongxiang Sun The metabolic resistance of Nilaparvata lugens to chlorpyrifos is mainly driven by the carboxylesterase CarE17 Ecotoxicology and Environmental Safety Brown planthopper Insecticide resistance Metabolic detoxification Homeobox Regulatory mechanism |
| title | The metabolic resistance of Nilaparvata lugens to chlorpyrifos is mainly driven by the carboxylesterase CarE17 |
| title_full | The metabolic resistance of Nilaparvata lugens to chlorpyrifos is mainly driven by the carboxylesterase CarE17 |
| title_fullStr | The metabolic resistance of Nilaparvata lugens to chlorpyrifos is mainly driven by the carboxylesterase CarE17 |
| title_full_unstemmed | The metabolic resistance of Nilaparvata lugens to chlorpyrifos is mainly driven by the carboxylesterase CarE17 |
| title_short | The metabolic resistance of Nilaparvata lugens to chlorpyrifos is mainly driven by the carboxylesterase CarE17 |
| title_sort | metabolic resistance of nilaparvata lugens to chlorpyrifos is mainly driven by the carboxylesterase care17 |
| topic | Brown planthopper Insecticide resistance Metabolic detoxification Homeobox Regulatory mechanism |
| url | http://www.sciencedirect.com/science/article/pii/S0147651322005784 |
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